APHIS PPQ

Pharr, TX, United States

APHIS PPQ

Pharr, TX, United States
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Gonzalez V.H.,University of Kansas | Smith-Pardo A.H.,APHIS PPQ | Engel M.S.,American Museum of Natural History | Engel M.S.,University of Kansas
Bulletin of the American Museum of Natural History | Year: 2017

We establish a new genus for an unusual species of Peruvian calliopsine bees (Panurginae: Calliopsini) that was initially reported in the literature as an undescribed species of Spinoliella Ashmead that purportedly expanded the range of the latter genus beyond Argentina and Chile. Although the new genus superficially resembles Spinoliella, it is easily distinguished by a unique combination of characters in both sexes but particularly in the male hidden metasomal sterna and genitalia. A cladistic analysis of 82 adult external morphological characters including all species of Spinoliella, as well as species of the remaining genera of Calliopsini, suggests that this group is sister to a clade consisting of Spinoliella and Callonychium Brèthes. We describe and illustrate Xeranthrena imponticula Gonzalez and Engel, new genus and species, from males and females collected in xeric areas along the Pacific slopes of the Peruvian Andes. In addition, the phylogenetic study suggests two well-defined clades within Spinoliella and corresponding to the previously recognized subgenera, although we do not advocate for their reinstatement. We briefly discuss new putative synapomorphies for Spinoliella and, building upon prior revisionary work, we describe and figure five new species: Spinoliella aidae Gonzalez, Smith-Pardo, and Engel, new species; S. confusa Gonzalez and Engel, new species; S. propinqua Gonzalez and Engel, new species; S. packeri Gonzalez and Engel, new species; and S. polita Gonzalez and Engel, new species. In addition, we synonymize S. karhadra Rodríguez, Toro, and Ruz under S. rufiventris Toro and Ruz (new synonymy). We provide new geographical and floral records, an identification keys to all 17 recognized species of Spinoliella, and updated key to the genera of Calliopsini. © American Museum of Natural History 2017.


Burke A.F.,Kansas State University | Leavengood J.M.,APHIS PPQ | Zolnerowich G.,Kansas State University
Zootaxa | Year: 2015

This checklist presents the distribution of the checkered beetle subfamily Tillinae (Coleoptera: Cleridae) in the New World. Information for 164 species and 2 subspecies from 11 genera is included. The data are based on an extensive survey of material collected throughout the Americas, descriptions of new species, a number of revisionary works, data from museum specimens, as well as unpublished checklists. Cymatodera, the most speciose tilline genus in the New World, has its greatest diversity in Mexico where 100 of the 134 recognized species are known to occur. Remaining genera inhabiting the New World and corresponding species numbers are: Araeodontia, 5 species; Barrotillus, 1 species; Bogcia, 2 species; Bostrichoclerus, 1 species; Callotillus, 5 species; Cylidrus, 1 species; Cymatoderella, 3 species; Lecontella, 3 species; Monophylla, 4 species; and Onychotillus, 5 species. An illustrated key to the genera of the New World Tillinae is provided. Forty-eight new country records are given for 35 species. References are presented for all species listed. Distribution maps for all New World genera are provided and locality data is presented for selected species. © 2015 Magnolia Press.


Nirmala X.,University of Florida | Nirmala X.,Center for Medical | Olson S.R.,APHIS PPQ | Holler T.C.,APHIS PPQ | And 2 more authors.
BioControl | Year: 2011

Field population surveillance of a targeted insect pest species is critical in evaluating management programs such as the sterile insect technique. Fluorescent powder dyes currently used to distinguish released tephritids from the field population are not optimal in terms of reliability and human health issues. Genetically transformed tephritid species present the possibility of using fluorescent transgenes for marking. Here we studied the stability of DsRed fluorescence in transgenic flies maintained in aqueous torula yeast borax and propylene glycol. DsRed was stable in both solutions for three weeks by visual microscopic observations and could be used to unambiguously distinguish them from non-fluorescent wild type flies. To compensate for any potential ambiguity in visual identification a diagnostic PCR was developed that could specifically amplify the exotic heterologous marker gene. Therefore, the use of sterile transgenic insect strains carrying stably integrated fluorescent protein marker genes in biologically-based control programs could greatly improve released fly identification in pest management programs. © 2011 International Organization for Biological Control (outside the USA).

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