Apeejay Stya University

Sohna, India
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The Penn State Research Foundation and Apeejay Stya University | Date: 2015-07-28

The present invention includes compounds useful in preventing or treating cancer in a subject in need thereof. The present invention also includes methods of preventing or treating cancer in a subject in need thereof by administering to the subject a therapeutically effective amount of a compound of the invention.

Manchanda S.,Delhi Institute of Pharmaceutical science and Research DIPSAR | Sahoo P.K.,Delhi Institute of Pharmaceutical science and Research DIPSAR | Majumdar D.K.,Apeejay Stya University
Der Pharmacia Lettre | Year: 2016

This work has been done with a motto to develop a simple, accurate, precise, reproducible and economic reverse phase HPLC method for Acetazolamide in bulk drug as well as in formulations. in the current developed method C8H column (250x4.6mm) was used as stationary phase and potassium dihydrogen phosphate buffer (pH 3), Acetonitrile & water in a ratio 30:20:50 as mobile phase. ICH guidelines were followed for method validation and different parameters studied include linearity range, system suitability, specificity, accuracy, precision, limit of detection (LOD), limit of quantification (LOQ), Robustness and Solution Stability. Forced degradation studies were also done by exposing the drug to different stress conditions (Photolytic, thermal, acidic, alkaline, & Oxidative). The developed validated method was also utilized to quantify the Acetazolamide in the marketed formulation successfully. The method was found linear within the range of 20-120μg/mL with LOD of 37.60ng/mL & LOQ of 0.11396 μg/mL whereas recovery was found within the range of 99.98%-100.77%. Method was found to be able to distinguish the parent drug from degraded products & quantify Acetazolamide in Dosage form (101.14%). A linear, robust & economic RP-HPLC method was developed with LOD & LOQ within the good range which can be adopted for the routine analysis of Acetazolamide in bulk drugs & formulations.

Yadav A.K.,National Dairy Research Institute | Tyagi A.,National Dairy Research Institute | Kaushik J.K.,National Dairy Research Institute | Saklani A.C.,Apeejay Stya University | And 2 more authors.
Microbiological Research | Year: 2013

Human feacal isolates were ascertain as genus Lactobacillus using specific primer LbLMA1/R16-1 and further identified as Lactobacillus plantarum with species specific primers Lpl-3/Lpl-2. 25 L. plantarum strains were further assessed for hydrophobicity following the microbial adhesion to hydrocarbons (MATH) method and colonization potentials based on their adherence to immobilized human collagen type-1. Surface proteins were isolated from selected L. plantarum 91(Lp91) strain. The purified collagen binding protein (Cbp) protein was assessed for its anti-adhesion activity against enteric Escherichia coli 0157:H7 pathogen on immobilized collagen. Four L. plantarum strains displayed high degree of hydrophobicity and significant adhesion to collagen. A 72. kDa protein was purified which reduced 59.71% adhesion of E. coli 0157:H7 on immobilized collagen as compared to control well during adhesion assay. Cbp protein is the major influencing factor in inhibition of E. coli 0157:H7 adhesion with extracellular matrix (ECM) components. Hydrophobicity and adhesion potential are closely linked attributes precipitating in better colonization potential of the lactobacillus strains. Cbp is substantiated as a crucial surface protein contributing in adhesion of lactobacillus strains. The study can very well be the platform for commercialization of indigenous probiotic strain once their functional attributes are clinically explored. © 2013 Elsevier GmbH.

Dhingra V.,Apeejay Stya University | Bhatia K.,YMCA University of Science and Technology
International Journal of Information Technology and Web Engineering | Year: 2012

Ontologies are the backbone of knowledge representation on Semantic web. Challenges involved in building ontologies are in terms of time, efforts, skill, and domain specific knowledge. In order to minimize these challenges, one of the major advantages of ontologies is its potential of "reuse," currently supported by various search engines like Swoogle, Ontokhoj. As the number of ontologies that such search engines like Swoogle, OntoKhoj Falcon can find increases, so will the need increase for a proper ranking method to order the returned lists of ontologies in terms of their relevancy to the query which can save a lot of time and effort. This paper deals with analysis of various ontology ranking algorithms. Based on the analysis of different ontology ranking algorithms, a comparative study is done to find out their relative strengths and limitations based on various parameters which provide a significant research direction in ranking of ontologies in semantic web. Copyright © 2012, IGI Global.

Mahajan R.,Manav Rachna International University | Mahajan R.,Apeejay Stya University | Bansal D.,Manav Rachna International University
ICROIT 2014 - Proceedings of the 2014 International Conference on Reliability, Optimization and Information Technology | Year: 2014

Electrocardiogram (ECG) is a primary clinical diagnostic tool for detection of cardiac arrhythmias. As ECG signals are generally acquired over longer time periods at extremely high resolution and thus are highly data intensive. This leads to the requirement of large storage space for database construction and more transmission bandwidth for remote ECG signal analysis, respectively. Successive ECG beats and sample values however, show some redundancy along with the information content. By removing this redundancy, ECG signal compression can be achieved. This paper comprises implementation of hybrid ECG signal compression system based on frequency transformation and parameter extraction techniques. It uses discrete cosine transform (DCT) and Fast Fourier transform (FFT) to compress the ECG signal. This compressed ECG signal is embedded with corresponding heart rate information in order to obtain a high quality reconstructed signal required for accurate cardiac state diagnosis. The proposed algorithm is tested for compression of bradycardia and tachycardia ECG rhythms selected from MIT-BIH arrhythmia database and the performance is evaluated using compression ratio and percent root-mean-square difference (PRD). The high compression ratio, low reconstruction error and less computational complexity justify the efficiency of hybrid techniques in ECG signal compression and thus in telecardiology © 2014 IEEE.

Jindal V.,Apeejay Stya University | Bawa S.,Thapar University | Batra S.,Thapar University
Information Processing and Management | Year: 2014

With ever increasing information being available to the end users, search engines have become the most powerful tools for obtaining useful information scattered on the Web. However, it is very common that even most renowned search engines return result sets with not so useful pages to the user. Research on semantic search aims to improve traditional information search and retrieval methods where the basic relevance criteria rely primarily on the presence of query keywords within the returned pages. This work is an attempt to explore different relevancy ranking approaches based on semantics which are considered appropriate for the retrieval of relevant information. In this paper, various pilot projects and their corresponding outcomes have been investigated based on methodologies adopted and their most distinctive characteristics towards ranking. An overview of selected approaches and their comparison by means of the classification criteria has been presented. With the help of this comparison, some common concepts and outstanding features have been identified. © 2013 Elsevier Ltd. All rights reserved.

Wang Y.,University of Arizona | Saha B.,Apeejay Stya University | Li F.,University of Arizona | Frett B.,University of Arizona | Li H.-Y.,University of Arizona
Tetrahedron Letters | Year: 2014

An expeditious cascade protocol for the synthesis of functionalized imidazo[1,2-a]pyridin-3-ols was developed based on the Petasis reaction. With the availability of commercial reagents and high efficiency in expanding molecule diversity, this methodology is superior to the existing procedures for the synthesis of imidazo-pyridin-3-ol analogues. © 2014 Elsevier Ltd. All rights reserved.

Kumar A.,Apeejay Stya University
E-Journal of Chemistry | Year: 2011

The triplex formation of disulphide containing oligonucleotide with duplex DNA using melting temperature studies is reported. The stability of the triplex formed with disulphide containing oligonucleotide was compared with unmodified oligonucleotide and C-5 propyne deoxyuridine containing oligonucleotide. Melting temperature (Tm) values of the triplexes formed with disulphide containing oligonucleotide were found to be 37 oC and 46 oC at pH 7 and 6 respectively. The triplexes formed with C-5 propyne deoxyuridine substituted oligonucleotide showed Tm values at 27 oC and 42 oC at pH 7 and 6. The Tm values of the triplexes formed with unmodified oligonucleotide were found to be 18 oC and 32 oC at pH 7 and 6 respectively. This clearly demonstrates that disulphide containing oligonucleotide stabilzes triplex DNA structure better than unmodified oligonucleotide as well as C-5 propyne deoxyuridine containing oligonucleotide when targeted with duplex DNA at both the mentioned pH values.

Kumar A.,Apeejay Stya University
E-Journal of Chemistry | Year: 2011

The small dumbbell oligonucleotides containing loops of phosphodiester (OL-1), two trimethylene, C3 moieties in each loop (OL-2) and phosphorothioate (OL-3) linkages were synthesized. Incubation of OL-1 and OL-2 with S-1 nuclease generated break down products whereas incubation of OL-3 did not result in significant cleavage. Their binding to moloney murine leukemia virus reverse transcriptase was evaluated by PAGE band mobility shift assays. The OL-3 bound more strongly to the reverse transcriptase than OL-1 and OL-2. The dissociation constants evaluated using PAGE band mobility shift assays were of the order of 10-7. Investigation of inhibition of RNase H activity of reverse transcriptase showed that the OL-3 is a better inhibitor of the retroviral RNase H activity than both OL-1 and OL-2. Thus OL- 3 may be used as RNase H inhibitor. Our studies demonstrated that this particularly designed oligonucleotide (OL-3) displays an IC50 of 25 nM in its inhibition on the reverse transcriptase RNase H activity, a magnitude lower than that of first nucleotide reverse transcriptase of HIV-1, tenofovir, introduced by Gilead Science in the market.

Manchanda S.,Delhi Institute of Pharmaceutical science and Research DIPSAR | Sahoo P.K.,Delhi Institute of Pharmaceutical science and Research DIPSAR | Majumdar D.K.,Apeejay Stya University
Nanotechnology Reviews | Year: 2016

Chitosan-dextran sulfate nanoparticles of acetazolamide were formulated by using the ionic gelation technique, and evaluated for different attributes like particle size, ζ potential, drug entrapment, particle morphology, in vitro drug release, and in vivo efficacy. Particle size was observed to be changed with the increment of the drug/polymer ratio. Sustained in vitro drug release was exhibited by the particulate formulation that followed the Korsmeyer-Peppas kinetic model. Drug release from nanoparticles was found to occur, as shown by the results, through a combination of dissolution and diffusion. The optimized formulation had a particle size of 172.3 nm and ζ potential of 36.46 mV. The particles had a spherical shape and polydispersity index of 0.257. Decrease in crystallinity of the drug was indicated by powder X-ray diffraction and differential scanning calorimetry studies, in the optimized nanoparticle formulation. Approximately 2.5 times higher transcorneal permeation of drug was observed across the excised goat cornea, in comparison to the aqueous solution of drug, without any corneal damage, during ex vivo transcorneal studies. In vitro mucoadhesion studies showed 91.59% mucoadhesion. The in vivo studies involving ocular hypotensive activity in rabbits revealed significantly higher hypotensive activity with a p-value of <0.05, in contrast to a plain drug solution, with no signs of ocular irritation. The stability studies showed that the formulation was rather stable. © 2016 Walter de Gruyter GmbH, Berlin/Boston 2016.

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