ApaTech Ltd | Date: 2015-04-29
A synthetic osteoinductive porous biomaterial is provided comprising: a network of interconnected micropores, wherein the microporosity is 23% by volume or more; wherein the surface free energy of the biomaterial is 19 mJ/m or more; and the mean interconnection diameter and the mean interconnection diameter and the surface free energy are chosen to provide a permeability resulting from the micropores of 0.206 nm2 or greater and a capillary pressure difference in water of 3.7 kPa or more. The biomaterial contains hydroxyapatite and silicon.
Apatech Ltd | Date: 2015-02-18
Pharmaceutical and veterinary preparations, namely, materials, namely, bone grafts composed of living material for biomedical applications, biomedical materials, namely, bone grafts composed of living material, bone implants composed of living material, bone grafts composed of living material, bone scaffolds composed of living material, bone adhesives for surgical purposes, bone coatings composed of living material, bone cement for surgical and orthopedic purposes, and surgical implants comprising living tissue for guided tissue regeneration in bone surgery and grafting; sanitary preparations for medical use; medical plasters, materials for dressings, namely, bandages and gauze; material for stopping teeth, dental wax; all purpose disinfectants; preparations for destroying vermin. synthetic materials, namely, synthetic bone implants for biomedical applications; biomedical materials, namely, synthetic bone implants; artificial bone implants; artificial bone grafts; artificial bone scaffolds; artificial bone coatings, namely, bone void fillers; bone setting machines and instruments.
University of Aberdeen and ApaTech Ltd | Date: 2014-05-09
The present invention provides a process for the preparation of a silicate and carbonate co-substituted calcium phosphate material. The process comprises the steps of: forming a silicon and optionally carbon-containing calcium phosphate precipitate by an aqueous precipitation method involving preparing an aqueous solution comprising phosphate ions, silicate ions, calcium ions and optionally carbonate ions, wherein the ratio of Ca/P and of Ca/(P+Si) in the solution is maintained above approximately 1.67; and heating the precipitate in an atmosphere comprising carbon and oxygen to form a silicate and carbonate co-substituted calcium phosphate material. The present invention also provides a synthetic carbonate and silicate co-substituted hydroxyapatite material, as well as a biomedical material.
Huang J.,University of Cambridge |
Best S.M.,University of Cambridge |
Bonfield W.,University of Cambridge |
Buckland T.,ApaTech Ltd.
Acta Biomaterialia | Year: 2010
Hydroxyapatite containing levels of titanium (TiHA) of up to 1.6 wt.% has been produced via a chemical co-precipitation route. The distribution of Ti was seen by transmission electron microscopy/energy-dispersive X-ray analysis to be uniform throughout as-prepared nanosized TiHA particles (20 nm × 100 nm). The incorporation of Ti into the HA structure was found to influence the ceramic microstructure on sintering and the grain size was found to decrease from 0.89 μm with HA to 0.63 μm with 0.8 wt.% TiHA (0.8 TiHA) and 0.45 μm with 1.6 wt.% TiHA (1.6 TiHA). Rietveld refinement analysis showed that there was a proportional increase in both the a and c axis with incorporation of Ti into the HA lattice structure, leading to an increase in the cell volume with the addition of Ti. Fourier transform-Raman analysis showed a slight increase in the ratio of O-H/P-O peaks on TiHA, in comparison with HA. A bone-like apatite layer was formed on the surface of TiHA after immersion in simulated body fluid for 3 days, which demonstrated the high in vitro bioactivity of TiHA. In vitro culture with primary human osteoblast (HOB) cells revealed that TiHA was able to support the growth and proliferation of HOB cells in vitro, with a significantly higher cell activity being observed on 0.8 TiHA after 7 days of culture in comparison with that on HA. Well-organized actin cytoskeletal protein was developed after 1 day of culture, and an increase in cell filopodia (attachment) was observed on TiHA sample surfaces. The results indicate that TiHA has great potential for biomedical applications. © 2009 Acta Materialia Inc.
Guth K.,Queen Mary, University of London |
Campion C.,ApaTech Ltd. |
Buckland T.,ApaTech Ltd. |
Hing K.A.,Queen Mary, University of London
Advanced Engineering Materials | Year: 2010
Hydroxyapatite (HA) is a well-established graft material used in bone repair. Silicon-substituted hydroxyapatite (SA; 0.8 wt% Si) has shown greater bone ingrowth and bone coverage than phase pure HA. To assess the effect of microporosity on sensitivity of cell attachment to surface physiochemistry, microporous SA and HA discs, and control Thermanox (TMX) discs were incubated with osteoblastlike cells (5-104 HOS-TE85 cells) under differing tissue culture conditions. To investigate early cellular attachment, organization, and differentiation, cells were also stained for integrin-α5 β1, actin, and runt-related transcription factor (RUNX-2), respectively, after incubation on HA, SA, and TMX discs for 3 days. No significant differences emerged between HA, SA, and TMX discs in mean numbers of cells attached in serum free medium (SFM) over 90 min incubation. In contrast, significantly more cells were attached to SA than HA after 180 min incubation in complete medium (C-MEM) containing fetal calf serum (p<0.05). Cell attachment to SA and HA discs pre-conditioned in SFM supplemented with fibronectin (FN) was lower than discs pre-conditioned in C-MEM, suggesting sensitivity of an active FN conformation to the presence of co-adsorbates. Confocal microscopy demonstrated significantly more co-localization of integrin αβ and actin on SA than HA. Translocalization of RUNX-2 to the nucleus was stronger in cells incubated on SA. Microporosity did not diminish the effect of surface physiochemistry on cell adhesion, and enhanced cell attachment for SA appears to be mediated by differences in the quality of adsorbed protein rather than via direct effects of substrate chemistry. © 2010 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.