Liu H.-J.,Anyang Tumor Hospital |
Zhang B.,Zhengzhou University
Hedianzixue Yu Tance Jishu/Nuclear Electronics and Detection Technology | Year: 2014
PET and SPECT is the most advanced nuclear medical imaging technology. GATE is a dedicated Monte Carlo simulation platform for PET and SPECT. This study validates two GATE models of Simens ECAT EXACT HR+ PET scanner and Simens PET/CT Biograph 2. After the simulation of PET systems completed, testing and performance evaluation of simulation systems were proceeded according to NEMA 2001 performance protocols, including spatial resolution, scatter fraction and sensitivity. Test results show that, performances of GATE simulation systems agree well with the experimental values.
Tian F.,Zhengzhou University |
Zhang C.,Xinxiang Medical University |
Tian W.,Anyang Tumor Hospital |
Jiang Y.,Zhengzhou University |
Zhang X.,Zhengzhou University
Oncology Reports | Year: 2012
The activation of the NF-κB signaling pathway plays a critical role in carcinogenesis. The role of the NF-κB pathway in esophageal squamous cell carcinoma (ESCC) remains ill-defined. The objective was to detect whether p65siRNA and curcumin could promote ESCC cell apoptosis and increase the sensitivity of ESCC cells to chemotherapeutic drugs by inhibiting the NF-κB signaling pathway, and to compared these two treatments. In the present study, the status of the NF-κB pathway, in the two ESCC cell lines Eca109 and EC9706, was analyzed and the ability of p65 siRNA and curcumin alone or in combination with 5-FU to modulate this pathway in vitro and in vivo was investigated. The results showed that the NF-κB signaling pathway in the ESCC cell lines was constitutively activated. Both p65 siRNA and curcumin mediated suppression of activation of the NF-κB signaling pathway via inhibition of the expression of p65 or IκBα phosphorylation in ESCC cell lines. The cells treated with combination of p65 siRNA or curcumin and 5-FU revealed a lower cell viability and higher apoptosis compared to those treated with 5-FU alone. In a human ESCC xenograft model, p65 siRNA or curcumin and 5-FU alone reduced the tumor volume, respectively, but their combination had the strongest anticancer effects. Curcumin was more effective than p65 siRNA in vitro and in vivo. Overall, our results indicate that the constitutively activated NF-κB signaling pathway plays a crucial role in these two ESCC cell lines and both p65siRNA and curcumin can promote ESCC cell apoptosis and enhance the sensitivity to 5-FU through suppression of the NF-κB signaling pathway. It is still a long time before RNA interference will be used in the clinic. Therefore, curcumin is proved to be useful in the treatment of ESCC as it is a pharmacologically safe compound without side effects.
Liu H.J.,Anyang Tumor Hospital |
Zhao S.J.,Zhengzhou University
Applied Mechanics and Materials | Year: 2014
PeneloPET is a PET-dedicated Monte Carlo simulation toolkit, which is based on PENELOPE. This article describes the characteristics and the general process of PeneloPET simulation. Then we compare the simulation results of PeneloPET and GATE to model the GE Healthcare eXplore Vista microPET system respectively, including sensitivity and noise equivalent count rate. The results show that PeneloPET simulation data corresponds with the data from real scanners and GATE simulation, and proves PeneloPET is an accurate toolkit for PET simulation. © (2014) Trans Tech Publications, Switzerland.
Wang F.,Zhengzhou University |
Wang N.,Anyang Tumor Hospital |
Zong H.,Zhengzhou University
Onkologie(Czech Republic) | Year: 2013
Background: Accumulating evidence has shown that microRNAs (miRNAs) are aberrantly expressed in human esophageal cancer and crucial to tumorigenesis. Herein, we identified the role of miR-145 in esophageal squamous cell carcinoma (ESCC) development in vitro and in vivo. Material and Methods: miR-145 expression was investigated in 40 ESCC samples as well as 5 ESCC cell lines by real-time polymerase chain reaction. Crystal violet and transwell assays were conducted to explore the effects of miR-145 on the proliferation and invasion of human ESCC cell lines, respectively. The impact of overexpression of miR-145 on putative target c-Myc was subsequently confirmed via Western blot. Results: miR-145 expression was frequently downregulated in ESCC specimens and cell lines compared with adjacent normal tissues (p < 0.05). Overexpression of miR-145 suppressed (p < 0.05) ESCC cell proliferation and invasion, as well as the growth of xenograft tumors in mice. Overexpression of miR-145 significantly decreased (p < 0.05) the protein level of c-Myc which has previously been identified as a direct target of miR-145. Conclusion: Our results demonstrate that overexpression of miR-145 inhibits tumor growth in part by targeting c-Myc. Our findings revealed that miR-145 may act as a tumor suppressor in ESCC, and its dysregulation may be involved in the initiation and development of human ESCC. © 2013 S. Karger GmbH, Freiburg.
Shen F.-F.,Xinxiang Medical University |
Zhou F.-Y.,Anyang Tumor Hospital |
Xue Q.-S.,Intensive Care Unit |
Pan Y.,Xinxiang Medical University |
And 5 more authors.
Molecular Biology Reports | Year: 2013
Cytochrome P4501A1 (CYP1A1) enzyme is a member of the CYP superfamily of enzymes. CYP1A1 A2455G and T3801C are two most commonly studied polymorphisms loci. Previous studies have reported that CYP1A1 polymorphisms increase esophageal cancer (EC) risk. However, the results remain controversial and ambiguous. To further investigate the association between CYP1A1 polymorphisms (A2455G and T3801C) and EC risk. A meta-Analysis was performed to investigate the association between CYP1A1 polymorphisms and EC risk. A total of 13 articles (A2455G and T3801C: 2 papers, A2455G: 8 papers, T3801C: 3 papers) from the PubMed containing information on the CYP1A1 polymorphisms and EC were included in this meta-Analysis, with summational sample size of 1,881 EC cases and 3,786 controls. Stratified analysis was performed to evaluate the ethnicity (Caucasians and Asian) and histopathology type (esophageal squamous cell carcinoma and esophageal adenocarcinoma) effect. No obvious publication bias in the two polymorphisms was observed. Our meta-Analysis revealed a significant association between the A2455G polymorphism and EC (OR = 1.55 per A allele, 95 % CI 1.29-1.85, P>0.001). Stratification analysis by ethnicity and histopathology type showed significant association in the population of Asian origin (OR = 1.55, 95 % CI 1.28-1.89, P>0.001) and in histopathology type of ESCC (OR = 1.40, 95 % CI 1.19-1.65, P>0.001). We didn't observe the significant association between CYP1A1 T3801C polymorphism and EC. We observed a difference of allele frequencies between Caucasian and Asian population in the meta-Analysis. The allele frequencies in our meta-Analysis were consistent with the allele frequencies in 1000 Genome Project. Our meta-Analysis demonstrated distinct evidence that CYP1A1 A2455G polymorphism was associated with the risk of EC.© Springer Science+Business Media Dordrecht 2013.