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Yanggu, South Korea

Cui H.-Y.,Chungbuk National University | Cui H.-Y.,Anti aging Research Institute | Niranjana Murthy H.,Chungbuk National University | Niranjana Murthy H.,Karnatak University | And 4 more authors.
CYTA - Journal of Food | Year: 2014

The present study was conducted with the objective of developing efficient method of extraction of nutraceutical compounds from protocorms of Dendrobium candidum for the food industry. Conventional heat reflux method was compared with ultrasonic-assisted method for extraction of polysaccharides, polyphenolics and flavonoids. Methanol, ethanol, acetone, acetonitrile and water were used as solvents for heat reflux extraction. Parameters tested for the optimization were methanol concentration (20, 40, 60, 80 and 100%) and duration of treatment (30 min, 1, 2, 4, 6 h). The treatment of samples with 80% methanol for 2-h period proved to be most suitable procedure for extraction of bioactive compounds. Heat reflux method was found superior to ultrasonic-assisted method since it yields higher amounts of bioactive compounds (463.0 mg g-1 DW polysaccharides, 10.75 mg g-1 DW polyphenolics and 5.30 mg g-1 DW flavonoids) and extracts obtained from heat reflux method also showed optimal antioxidant activity. © 2014 Taylor & Francis.

Cui H.-Y.,Chungbuk National University | Murthy H.N.,Chungbuk National University | Murthy H.N.,Karnatak University | Moh S.H.,Anti aging Research Institute | And 3 more authors.
Biochemical Engineering Journal | Year: 2014

Protocorm cultures of Dendrobium candidum were established in balloon type bubble bioreactors using Murashige and Skoog (MS) medium with 0.5mgl-1 α-naphthaleneacetic acid (NAA), 2.5% (w/v) sucrose, 5:25mM NH4:NO3 and 1% (v/v) banana homogenate for the production of biomass and bioactive compounds. In 3l bioreactor containing 2l medium, a maximum protocorm biomass (21.0gl-1 dry biomass) and also optimum quantities of total polysaccharides (389.3mgg-1 DW), coumarins (18.0mgg-1 DW), polyphenolics (11.9mgg-1 DW), and flavonoids (4.5mgg-1 DW) were achieved after 7 weeks of culture. Based on these studies, 5 and 10l bioreactor cultures were established to harvest 80g and 160g dry biomass. In 10l bioreactors, the protocorms grown were accumulated with optimal levels of polysaccharides (424.1mgg-1 DW), coumarins (15.8mgg-1 DW), polyphenols (9.03mgg-1 DW) and flavonoids (4.7mgg-1 DW). The bioreactor technology developed here will be useful for the production of important bioactive compounds from D. candidum. © 2014 Elsevier B.V.

Cui H.-Y.,Chungbuk National University | Cui H.-Y.,Anti aging Research Institute | Murthy H.N.,Chungbuk National University | Murthy H.N.,Karnatak University | And 4 more authors.
Industrial Crops and Products | Year: 2014

Protocorm suspension cultures of Dendrobium candidum were established in balloon type bubble bioreactors for the production of biomass and bioactive compounds. Various culture parameters namely culture method, inoculum density and aeration volume were optimized. Immersion cultures found to be superior as compared to raft and ebb and flood methods in terms of accumulation of optimum biomass and bioactive compounds. An inoculums density of 50gl-1 was found suitable for accumulation of biomass and bioactive compounds. An aeration volume of 0.1vvm was found suitable for the accumulation of biomass, whereas aeration volume of 0.3vvm was found suitable for production of polysaccharides, coumarins, polyphenolics, flavonoids, vitamin C and vitamin E. The results of the current study are useful for scale-up process for the production of bioactive compounds from D. candidum protocorm suspension cultures. © 2013 Elsevier B.V.

Suh S.-S.,South Sea Research Institute | Park M.,South Sea Research Institute | Hwang J.,South Sea Research Institute | Lee S.,Sungkyunkwan University | And 3 more authors.
Ocean Science Journal | Year: 2014

Understanding the patterns of microbial diversity and their composition in seawater is necessary to assess the impacts of microbes on marine ecosystem. Although the potential roles of microbes in the ocean have been studied, their diversity and relationship with environmental factors remain unclear. In this study, we adopted a 16S rRNA gene tag-pyrosequencing technique to investigate the bacterial communities associated with two different water masses from Tongyoung in the South Sea of Korea. Our results revealed highly diverse bacterial communities up to 900 Operational Taxonomic Units (OTUs) estimated from each seawater which was collected in the month of March and May, when the environmental conditions including temperature differed significantly: 7.2°C and 17.6°C in March and May, respectively. Altogether, 13 bacterial phyla were recovered from the seawater, of which Proteobacteria was the most dominant group. In addition, the value of the Shannon index, which measures the evenness of the distribution of individuals among OTUs, in May is higher than that in March, indicating that it displays a wider diversity of bacteria. Interestingly, the proportion of pathogenic bacteria was significantly increased in the month of May compared to March, suggesting that pathogenic bacteria were increasingly emerging in May. In particular, Pseudoalteromonas and Vibrio spp. were determined as major pathogenic bacteria from both water masses, of which Vibrio spp. were dominant. © KSO, KIOST and Springer 2014.

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