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Busan, South Korea

Ko Y.-J.,Gyeongsang National University | Jeong J.-W.,Daegu Gyeongbuk Institute of Science and Technology | Choi Y.-H.,Korea University | Choi Y.-H.,Anti Aging Research Center | Ryu C.-H.,Gyeongsang National University
Molecular Medicine Reports | Year: 2013

Previous studies have suggested that soy sauce contains specific bioactive components and various biological activities of soy sauce have been observed. Soy soluble polysaccharide (SSPS), a predominant bioactive compound in soy sauce, has numerous pharmacological actions, including anti-inflammatory and immunomodulating activities. In the current study, the apoptotic effects of SSPS were investigated in HCT-116 human colon cancer cells. Treatment with SSPS significantly inhibited cell growth in a concentration-dependent manner by inducing apoptosis but not necrosis. This induction was associated with the generation of reactive oxygen species (ROS), mitochondrial dysfunction, activation of caspases and cleavage of the poly (ADP-ribose) polymerase protein. Induction of apoptotic cell death of HCT-116 cells by SSPS showed a correlation with the downregulation of members of the inhibitor of apoptosis protein family, including X-linked inhibitor of apoptosis protein and antiapoptotic Bcl-2, and upregulation of Bax and Bad. Administration of N-acetyl-L-cysteine, a scavenger of ROS, significantly decreased SSPS-induced apoptosis. These results indicate a critical role of signaling cascades involving a ROS-mediated caspase pathway in the anticancer effects of SSPS. Source

Lee H.-H.,Daegu Gyeongbuk Institute of Science and Technology | Han M.H.,Dong - A University | Han M.H.,Anti Aging Research Center | Hwang H.J.,Anti Aging Research Center | And 7 more authors.
International Journal of Molecular Medicine | Year: 2015

Diallyl trisulfide (DATS; di-2-propen-1-yl trisulfide) is an organic polysulfide compound found in garlic and other allium vegetables. Although certain studies have demonstrated that DATS possesses strong anti-inflammatory activity, the underlying molecular mechanisms remain largely unresolved. In the present study, the anti-inflammatory potential of DATS was investigated using the murine macrophage RAW 264.7 cell model. At non-toxic concentrations, DATS inhibited the production of nitric oxide (NO) and prostaglandin E2 by inhibiting inducible NO synthase and cyclooxygenase-2 expression at the transcriptional level in lipopolysaccharide (LPS)-activated RAW 264.7 macrophages. DATS attenuated the release of the pro-inflammatory cytokines, tumor necrosis factor-α and interleukin-1β, by inhibiting mRNA expression, respectively. DATS also suppressed LPS-induced DNA-binding activity of nuclear factor-κB (NF-κB), as well as the nuclear translocation of the NF-κB p65, which correlated with the inhibitory effects of DATS on inhibitor-κB (IκB) degradation. In addition, DATS was observed to significantly suppress LPS-induced Toll-like receptor 4 (TLR4) and myeloid differentiation factor 88 expression and the binding of LPS to macrophages, indicating the antagonistic effect of DATS against TLR4. Furthermore, blocking TLR4 signaling with the specific TLR4 signaling inhibitor, CLI-095, increased the anti-inflammatory potential of DATS in LPS-stimulated RAW 264.7 macrophages. These data demonstrate that DATS may attenuate the initiation of LPS-mediated intracellular signaling cascades by suppressing activation of NF-κB and by inhibiting binding of LPS to TLR4 on macrophages. © International Journal of Molecular Medicine 2014. Source

Lee H.H.,Dong - A University | Park C.,Dong - A University | Park C.,Anti Aging Research Center | Jeong J.-W.,Dong - A University | And 11 more authors.
International Journal of Oncology | Year: 2013

Cordycepin is the main functional component of Cordyceps militaris, which has been widely used in oriental traditional medicine. This compound has been shown to possess many pharmacological properties, such as enhancing the body's immune function, and anti-inflammatory, anti-aging and anticancer effects. In the present study, we investigated the apoptotic effects of cordycepin in human prostate carcinoma cells. We found that treatment with cordycepin significantly inhibited cell growth by inducing apoptosis in PC-3 cells. Apoptosis induction of PC-3 cells by cordycepin showed correlation with proteolytic activation of caspase-3 and -9, but not caspase-8, and concomitant degradation of poly (ADP-ribose) polymerases, collapse of the mitochondrial membrane potential (MMP). In addition, cordycepin treatment resulted in an increase of the Bax/Bcl-2 (or Bcl-xL) ratio, downregulation of inhibitor of apoptosis protein (IAP) family members, Bax conformational changes, and release of cytochrome c from the mitochondria to the cytosol. The cordycepin-induced apoptosis was also associated with the generation of intracellular reactive oxygen species (ROS). However, the quenching of ROS generation with antioxidant N-acetyl-L-cysteine conferred significant protection against cordycepin-elicited ROS generation, disruption of the MMP, modulation of Bcl-2 and IAP family proteins, caspase-3 and -9 activation and apoptosis. This indicates that the cellular ROS generation plays a pivotal role in the initiation of cordycepin-triggered apoptotic death. Collectively, our findings suggest that cordycepin is a potent inducer of apoptosis of prostate cancer cells via a mitochondrial-mediated intrinsic pathway and that this agent may be of value in the development of a potential therapeutic candidate for both the prevention and treatment of cancer. Source

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