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Aydin O.,Antalya Education and Research Hospital | Yacinkaya S.,Antalya Education and Research Hospital | Eren E.,Antalya Public Health Center | Ergin M.,Ataturk Education and Research Hospital | And 2 more authors.
Clinical Laboratory

Background: It is difficult to think of a disease in the etiology of which free radicals would not be involved. The human body has a number of endogenous free-radical scavenging systems to avoid harm that can lead to any disease including cancer. This study aims to measure the total anti-oxidative status (TAS), in particular the activities of HDL-associated anti-oxidative enzymes paraoxonase (PON1), arylesterase (ARE), and total thiol levels (Ttl) in bladder cancer (BC) patients for the first time in literature. Methods: Forty two male patients (mean age, 66.6 ± 12.7 years) with BC who had presented at the Urology Outpatient Clinic were prospectively included in the study. Forty age- and gender-matched healthy control subjects (mean age 65 ± 7.4 years) were also enrolled for comparison. Analysis of PON1, ARE activities, measurement of TAS, and Ttl of serum were carried out using colorimetric measurement methods. Statistical analyses were performed using the MedCalc statistical software program. Results: ARE enzyme activity and Ttl levels were significantly lower in patients with BC compared to controls (p = 0.03; p = 0.02, respectively), whereas PON1 enzyme activity and TAS did not show significant differences. Ttl levels were lower in patients with a high grade cancer compared to those with a low grade cancer (p = 0.03). Conclusions: Anti-oxidant measurements might be essential in routine clinical use, not only as cancer disease markers but also as major actors in the management of anti-oxidant remedies in the very near future. Our findings showed that determination of ARE enzyme activity and Ttl levels were more precise than PON1 enzyme activity or TAS measurements, particularly in BC patients. When composing a marker panel, it should be kept in mind that determination of anti-oxidant levels based merely on TAS or PON1 activity may be deceptive and must include ARE enzyme activity and/or Ttl measurements. However, long-term clinical studies are needed to clarify the pathophysiological role of serum anti-oxidative levels and HDL-associated PON1 activity in BC patients. Source

Ellidag H.Y.,Central Laboratories of Antalya Education and Research Hospital | Eren E.,Antalya Public Health Center | Aydin O.,Central Laboratories of Antalya Education and Research Hospital | Yldrm M.,Oncology Clinic | And 2 more authors.
Medical Principles and Practice

Objective: To investigate the status of the oxidant/antioxidant balance in patients with multiple myeloma compared to healthy controls. Materials and Methods: This study was conducted on 40 multiple myeloma patients and 40 healthy controls of matched age and sex. Serum total thiol, oxidative stress index (OSI), total oxidant status (TOS), and total antioxidant status (TAS) were measured using colourimetric methods; paraoxonase-1 and arylesterase enzyme activities were also quantified. Results: Serum paraoxonase-1 and arylesterase activities and total thiol levels were significantly lower (p = 0.0001, p = 0.036 and p < 0.0001, respectively), whereas TOS and OSI levels were significantly higher (p < 0.0001 for both parameters) in multiple myeloma patients compared to controls. However, no significant differences in TAS were identified when the two groups were compared. Conclusions: Our findings indicate an impaired oxidative/antioxidative balance in multiple myeloma. We recommend further studies with larger groups to investigate the possible relationship between oxidative stress and the aetiopathogenesis of multiple myeloma. © 2013 S. Karger AG, Basel. Source

Ellidag H.Y.,Clinical Research Laboratories | Eren E.,Antalya Public Health Center | Aydin O.,Clinical Research Laboratories | Neselioglu S.,Ankara Education Research Hospital | Yilmaz N.,Clinical Research Laboratories
Journal of Medical Biochemistry

Background: Human serum paraoxonase-1 (PON1) shows wide variation among different ethnic groups around the world. The aim of the present study was to determine the phenotype distribution and enzymatic activity of PON1 and ARE (arylesterase) in colorectal cancer (CRC), bladder cancer (BC) and multiple myeloma (MM) patients compared to healthy subjects. Methods: A total of 160 subjects (40 CRC patients, 40 BC patients, 40 MM patients and 40 healthy controls) were admitted to the study. The phenotype distribution of PON1 was determined by using the dual substrate (paraoxon and phenylacetate) method. Results: PON 1 and ARE activities were significantly lower in the cancer patients compared to the control group. The following phenotype distributions were assessed in the cancer and control groups: MM: 52.5% (QQ), 40% (QR), 7.5% (RR); CRC: 52.5% (QQ), 40% (QR), 7.5% (RR); BC: 55% (QQ), 35% (QR), 10% (RR); and controls: 40% (QQ), 57.5% (QR), 2.5% (RR). Conclusions: We found that MM, CRC and BC patients were associated with lower PON1, ARE and stPON1 enzyme activities compared to the healthy subjects. However, PON1 phenotypes were similar between the cancer groups and control group. Source

Cerit N.,Antalya Education and Research Hospital | Onuk A.A.,Antalya Education and Research Hospital | Ellidag H.Y.,Antalya Education and Research Hospital | Eren E.,Antalya Public Health Center | And 2 more authors.
Advances in Clinical and Experimental Medicine

Background. Long-term occupational exposure to trace concentrations of volatile anesthetics is known to have adverse effects on the health of exposed personnel. Objectives. We investigated paraoxonase-1 (PON1) and arylesterase (ARE), as well as antioxidant status (TAS) and total oxidant status (TOS) levels in anesthesia personnel (AP) who were chronically exposed to inhalation anesthetics, and compared them with levels in a control group. Material and Methods. We designed a comparative prospective study with 50 female subjects. The first cohort included 25 full-time female workers in operating rooms in two locations in the Antalya Education and Research Hospital in Antalya, Turkey. The control group was comprised of 25 female individuals working in the same hospitals without any work-related exposure to hazardous agents. Results. Serum ARE activity and TAS levels were significantly reduced (p = 0.04 and p < 0.0001, respectively), whereas TOS and OSI levels were found to be significantly higher (p = 0.01 and p < 0.0001, respectively) in AP. However, there were no significant differences in PON1 activity, PON1/HDL-C, ARE/HDL-C, and PON1//ARE (p = 0.30, p = 0.5, p = 0.1 and p = 0.7, respectively) when the two groups were contrasted. Conclusions. According to the results of this study, depending on the putative role of PON/ARE in oxidant stress-related diseases, particularly atherosclerosis and cancer, AP might be considered a risk group for the development of atherosclerosis and many other diseases. © Copyright by Wroclaw Medical University. Source

Yilmaz N.,Central Laboratories of Antalya Education and Research Hospital | Aydin O.,Central Laboratories of Antalya Education and Research Hospital | Yegin A.,Central Laboratories of Antalya Education and Research Hospital | Tiltak A.,Neurology clinic of Antalya Education and Research Hospital | Eren E.,Antalya Public Health Center
Clinical Biochemistry

Objectives: There is strong evidence associating migraine with a variety of comorbid disorders, including cardiovascular disease and stroke, in which oxidative stress seems to be an important underlying mechanism. The aim of the study was to investigate in migraineurs the body oxidant/antioxidant balance and paraoxonase enzyme activities as a measure of HDL functionality. Design and methods: Oxidative stress index, total oxidant status and antioxidant status were examined in addition to the paraoxonase and arylesterase enzyme activities in sixty-two migraineurs and fifty healthy control subjects. Results: Serum arylesterase activities were significantly lower in migraineurs (p = 0.0065), whereas total oxidant status was higher in patients compared to the controls (p = 0.0035). Conclusions: This preliminary study showed that oxidative/antioxidative balance shifted towards the oxidative status in migraine. Moreover, the results also suggested that decreased arylesterase activities might be associated with HDL-related disfunction. © 2011 The Canadian Society of Clinical Chemists. Source

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