Investigation of nasal staphylococcus aureus carriage and methicilin resistantance rates with three different methods in food handlers working at Antalya [Antalya ili gida çalişanlarinda nazal staphylococcus aureus taşiyiciliǧinin ve mrsa oranlarinin üç farkli yöntem kullanilarak incelenmesi]
Sepin-Ozen N.,Antalya Halk Sagligi Laboratuvari |
Tuglu-Ataman S.,Antalya Halk Sagligi Laboratuvari |
Seyman D.,Antalya Egitim Arastirma Hastanesi |
Aldag H.,Antalya Halk Sagligi Laboratuvari |
Emek M.,Antalya Halk Sagligi Laboratuvari
Turk Hijyen ve Deneysel Biyoloji Dergisi | Year: 2013
Objective: Nasal carriage of Staphylococcus aures is increasingly reporting from all over the world. S.aureus is known for causing food poisoning through the production of enterotoxins. Asymptomatic carriage of S.aureus especially in food handlers can be the source of food poisining and transmission of this bacterium to other susceptible persons. Generally staphylococcal food poisoning is self-limiting process but can lead to serious infections on immunocompromised patients. According to the increase of methicilin resitance rates determinig of nasal carriage becomes an important process on health care settings. A growing concern is the emergence of MRSA infections in patients with no apparent risk factors. In this study we aimed to determine nasal carriage of S.aureus and methicilin resistance rates with BBL CHROMAGAR MRSA II medium and oxacilin and cefoxitin disc diffusion test. Methods: Between September 2009 and April 2010, 15.600 nasal cultures were taken from food handlers working at different region of Antalya during their routine carrier inspection process in Antalya Hifzissihha Institute (ANHEM). S.aureus isolates were identified with traditional biochemical techniques. Methicilin resistantance were determined with disc diffusion method using cefoxitin and oxacilin discs according to CLSI (Clinical Laboratory Standarts Institute) recommendations and also isolates were recultured on BBL CHROMAGAR MRSA II medium. The sensitivity and specificity of chromogenic medium was determined Results: A total of 526 (3,37%) S.aureus strains were isolated from 125 (23.8%) female and 401 (76.2%) male food handlers. 28 strains were detected as MRSA (5.3%). The sensitivity and specificity of BBL CHROMAGAR MRSA II medium was found 85.72% and 99% respectively. Conclusion: Healthy carriers of S.aureus strains have an important role in the dissemination of this bacterium. Nasal carriage rate of S.aureus in food handlers remains high. Determination of nasal carriage rate in food handlers with regular periodichealth examination which reduces the risk of transmission at food production-consumption chain is one of the most effective ways to preventfoodbornediseases.