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Aykal G.,Biyokimya Laboratory | Atakan-Erkal F.,Antalya Halk Sagligi Laboratuvari | Yegin A.,Biyokimya Laboratory | Eren E.,Biyokimya Laboratory | Yilmaz N.,Biyokimya Laboratory
Turk Hijyen ve Deneysel Biyoloji Dergisi | Year: 2016

Objective: HPLC (High Performance Liquid Chromatography) is the most commonly used method to differentiate the subtypes of hemoglobin in screening for hemoglobinopathy which is a major public health care problem especially in Mediterranean region. Today, different models and trademarks of HPLC equipment have been used in screening for hemoglobinopathy. However, the agreement of the results of the analyses performed by these types of equipment are unknown. In this study, the aim was to investigate the agreement between the results of the analyses of HbA2 values with D-10TM Hemoglobin testing system (BioRad,Hercules,USA) and Tosoh HLC 723 G8 (TosohBioscience, Japan) trademark. Methods: A total of 30 individuals, 12 males and 18 females, applied to the Antalya Training and Research Hospital laboratory between January 1 and January 31, 2016 for thalassemia screening were included to this study. Statistical correlations of hemoglobin A2 (HbA2) values, analyzed in each analyzer as a single analysis from each sample, were evaluated using two-way random intraclass correlation coefficient (ICC), Cohen's kappa coefficient, correlation coefficient (r) and Bland Altman analysis. Results are presented with graphics. Results: As a result of the analysis, 14 of the samples (46.66%) were detected as - thalassemia carrier with Tosoh HLC 723 G8 system and nine of the samples (30%) were detected as - thalassemia carrier with BioRad D-10TM system. There was significant differance in HbA2 values measured with both systems (z= -4.487; p < 0.001). For HbA2 values; intraclass correlation coefficient was 0.898, coefficeint of concordance was 0.783, Spearman corelation coefficiency was 0.944, Cohen's Kappa value 0.658 and Bland Altman analysis bias value was 0.75 (LowerLimit: -0.49; Upper Limit: 1.99). Conclusion: According to the regression, correlation and other statistical analyses, agreement between the results of HbA2 values measured by the two analyzers were found to be insufficient.

Sepin-Ozen N.,Antalya Halk Sagligi Laboratuvari | Tuglu-Ataman S.,Antalya Halk Sagligi Laboratuvari | Seyman D.,Antalya Egitim Arastirma Hastanesi | Aldag H.,Antalya Halk Sagligi Laboratuvari | Emek M.,Antalya Halk Sagligi Laboratuvari
Turk Hijyen ve Deneysel Biyoloji Dergisi | Year: 2013

Objective: Nasal carriage of Staphylococcus aures is increasingly reporting from all over the world. S.aureus is known for causing food poisoning through the production of enterotoxins. Asymptomatic carriage of S.aureus especially in food handlers can be the source of food poisining and transmission of this bacterium to other susceptible persons. Generally staphylococcal food poisoning is self-limiting process but can lead to serious infections on immunocompromised patients. According to the increase of methicilin resitance rates determinig of nasal carriage becomes an important process on health care settings. A growing concern is the emergence of MRSA infections in patients with no apparent risk factors. In this study we aimed to determine nasal carriage of S.aureus and methicilin resistance rates with BBL CHROMAGAR MRSA II medium and oxacilin and cefoxitin disc diffusion test. Methods: Between September 2009 and April 2010, 15.600 nasal cultures were taken from food handlers working at different region of Antalya during their routine carrier inspection process in Antalya Hifzissihha Institute (ANHEM). S.aureus isolates were identified with traditional biochemical techniques. Methicilin resistantance were determined with disc diffusion method using cefoxitin and oxacilin discs according to CLSI (Clinical Laboratory Standarts Institute) recommendations and also isolates were recultured on BBL CHROMAGAR MRSA II medium. The sensitivity and specificity of chromogenic medium was determined Results: A total of 526 (3,37%) S.aureus strains were isolated from 125 (23.8%) female and 401 (76.2%) male food handlers. 28 strains were detected as MRSA (5.3%). The sensitivity and specificity of BBL CHROMAGAR MRSA II medium was found 85.72% and 99% respectively. Conclusion: Healthy carriers of S.aureus strains have an important role in the dissemination of this bacterium. Nasal carriage rate of S.aureus in food handlers remains high. Determination of nasal carriage rate in food handlers with regular periodichealth examination which reduces the risk of transmission at food production-consumption chain is one of the most effective ways to preventfoodbornediseases.

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