Anqing Medical and Pharmaceutical College

Anqing, China

Anqing Medical and Pharmaceutical College

Anqing, China
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Tan Y.-J.,Anqing Medical and Pharmaceutical College | Dong R.-R.,China Pharmaceutical University | Hong H.,China Pharmaceutical University
Chinese Journal of Pharmacology and Toxicology | Year: 2016

OBJECTIVE To investigate the effect of pranlukast (Pran) on learning and memory impairment and neuroinflammatory and apoptotic response in streptozocin(STZ)-induced type 1 diabetic mice. METHODS Male ICR mice were injected through the tail vein with STZ (150 mg-kg-1) to induce the type 1 diabetes model. Diabetic mice were administered orally with Pran. After 4 consecutive weeks of administration, the escape latency in hidden platform trials, number of platform crossings and time spent in the target quadrant of mice were assessed by the Morris water maze (MWM) test. Western blot was used to detect the proteins of cysteinyl-leukotrienes receptor-1 (CysLTiR) and pro-inflammatory factors, nuclear factor-KB p65 subunit (NF-kB p65), interleukin-1β (IL-1 beta;, tumor necrosis factor-oc (TNF-α), and cleaved caspase 3, Bax and Bcl-2 in the hippocampus and prefrontal cortex of diabetic mice. We also determined fasting blood glucose, serum insulin and lipids such as triglyceride, total cholesterol, high density lipoprotein cholesterol, and low density lipoprotein cholesterol. RESULTS The data of the MWM test showed that untreated diabetic mice displayed a higher escape latency in hidden platform trials(P<0.05), and a smaller number of platform crossings (P<0.05) as well as shorter percentage of time spent in the target quadrant (P<0.05). The data of Western blotting showed that treatment with Pran 0.6 and 1.2 mg-kg-1 significantly reduced the levels of CysLT1R, nuclear NF-kB p65, IL-1 β and TNF-α, cleaved caspase 3, and the ratio of Bax and Bcl-2 in the hippocampus and prefrontal cortex of diabetic mice(P<0.05). However, Pran did not improve the fasting blood glucose, serum insulin or lipid metabolism disorder in diabetic mice. CONCLUSION Pran improves memory impairment and nerve injury in STZ-induced type 1 diabetic mice.

Chen F.-J.,Anqing Medical and Pharmaceutical College | Li J.,Anhui Medical University
Chinese Pharmacological Bulletin | Year: 2013

Aim: To observe the effect of edaravone on the cytochrome P450 2E1 (CYP 2E1) in immune liver injury model of mice induced by concanavalin A (Con A) Methods: After edaravone was injected, the mice were sensitized by Con A via intraperitoneal injection and acute immune liver damage was produced. The liver pathological changes were observed, serum ALT, AST concentration, the levels of MDA, SOD and GSH in liver were measured by spectrophotometry. Expression of CYP 2E1 in liver was detected by reverse transcription-polymerase chain reaction (RT-PCR) assay and Western blot. Results: Compared the model group, edaravone could reduce serum ALT, AST concentration; it also reduced MDA level, and raised SOD, GSH activities in liver homogenate. Edaravone treatment significantly decreased CYP2E1 mRNA level and CYP 2E1 protein expression in mouse liver tissues. Conclusion: In acute immune liver injury of mice induced by Con A, edaravone has certain protective effect, which it may be related with reducing CYP 2E1 expression, eliminating free radicals and lipid peroxidation, and enhancing the antioxidant capacity.

Mao X.-M.,Anqing Medical and Pharmaceutical College
Chinese Journal of Pharmaceutical Biotechnology | Year: 2014

Owing to the inability of transporting drug across the blood-brain barrier (BBB), the chemotherapy for brain neuroglioma has been limited. For overcoming these hurdles, the dual brain targeting liposomes (DBTL) were developed by conjugating with transferrin (TF) and chlorotoxin for transporting drug across the BBB and then targeting brain glioma. Prepare plasmid-loaded liposomes by reverse phase evaporation technique and demonstrate its physic-chemical properties, the encapsulation efficiency (EE) and conjugate efficiency (CE) were detected by column chromatograpic method and spectrophotometric method, respectively; the average diameter, distribution (PDI) and Zeta potential of liposomes were studied by dynamic light scattering. The EE, CE for transferrin (Tf) and chlorotoxin (CTX) were (90.0 ± 5.1)%, (41.5 ± 6.1)% and (50.2 ± 8.3)%, respectively. The average size was (219.9 ± 7.4) nm and Zeta potential was (21.0 ± 5.2)) mV. The results suggested that the reverse phase evaporation technique is feasible, repeatable, high encapsulation efficient and stable, which might make DBTL have the potential to overcome some of the major shortcomings of conventional chemotherapeutic strategies.

Jiang G.,Anhui University | Hu Y.,Anhui Medical University | Hu Y.,No 105 Hospital Of Pla | Liu L.,No 174 Hospital Of Pla | And 4 more authors.
Neurochemistry International | Year: 2014

Although the etiology of PD remains unclear, increasing evidence has shown that oxidative stress plays an important role in its pathogenesis and that of other neurodegenerative disorders. The phenolic glucoside gastrodin, a main constituent of a Chinese herbal medicine Gastrodia elata (GE) Blume, has been known to display antioxidant activity. The present study aimed to investigate the protective effects of gastrodin on 1-methyl-4-phenylpyridinium (MPP +)-induced oxidative cytotoxicity in human dopaminergic SH-SY5Y cells and the underlying mechanism for this neuroprotection. Results indicate that pre-treatment with gastrodin for 1 h significantly reduced the MPP +-induced viability loss, apoptotic rate and attenuated MPP +-mediated ROS production. In addition, gastrodin inhibited MPP +-induced lowered membrane potential, decreased Bcl-2/Bax ratio. Moreover, we have revealed the gastrodin increased Nrf2 nuclear translocation, which is upstream of heme oxygenase-1 (HO-1) expression and for the first time revealed gastrodin could increased antioxidant enzyme HO-1 expression in concentration-dependent and time-dependent manners. HO-1 siRNA transfection was employed, and confirmed gastrodin could active the expression of HO-1. And the increase in HO-1 expression was correlated with the protective effect of gastrodin against MPP+-induced injury. Because the inhibitor of HO-1 activity, ZnPP reversed the protective effect of gastrodin against MPP +-induced cell death. We also demonstrated that the specific p38 MAPK inhibitor, SB203580, concentration-dependently blocked on gastrodin-induced HO-1 expression, and meanwhile SB203580 reversed the protective effect of gastrodin against MPP+-induced cell death. Taken together, these findings suggest that gastrodin can induce HO-1 expression through activation of p38 MAPK/Nrf2 signaling pathway, thereby protecting the SH-SY5Y cells from MPP+-induced oxidative cell death. Thus our study indicates that gastrodin has a partial cytoprotective role in dopaminergic cell culture systems and could be of importance for the treatment of PD and other oxidative stress-related diseases. © 2014 Elsevier Ltd. All rights reserved.

Wang M.,Anqing Medical and Pharmaceutical College | Zhao K.,Anqing Teachers College | Wang R.,Anqing Medical and Pharmaceutical College
Zhongguo Zhongyao Zazhi | Year: 2012

Through studies on morphological characteristics, distribution and ecological habitat described in ancient literatures, Osmunda japonica is believed to the original plant of Chinese herbal medicine Cyrtomium Rhizome. Meanwhile, analysis is also made on causes for descriptions that do not comply with characteristics of O. japonica such as toxicity, flowers and fruits, illustrations, indumentums and flakes as well as appearance of other original plants. It is suggested to list O. japonica as the only original plant of Cyrtomium Rhizome and distinguish it from other frequently seen medical plants. Separated studies are also conducted for pesticide effect and active ingredients of Dryoteris Crassrhizomae Rhizome and Woodwardia japonica that show better effects.

Cao L.-L.,Anhui Medical University | Cao L.-L.,Huainan Union University | Li W.-Z.,Anhui Medical University | Si X.-L.,Anhui Medical University | And 3 more authors.
Zhongguo Zhongyao Zazhi | Year: 2013

Objective: To study the protective effect of astragaloside IV (AS IV) on H2O2 induced human mesangial cells (HMC), and further explore its molecular mechanism. Method: The cultured mesangial cells were divided into 5 groups: the normal control group, the H2O2 model group, the AS IV (12.5, 100 nmol · L-1) group and the Tempol (1 × 105 nmol · L-1) group. The MTT method was used to observe cell viability. Hoechst 33258 staining was used to observe the HMC apoptosis and DHE staining was used to detect the generation of reactive oxygen species (ROS). The flow cytometry was used to detect the changes in cell cycle. Western blot was used to detect the expression of Cyclin D1, CyclinA, p38, and T-p38. Result: H2O2 (1 × 105, 2 × 105, 3 × 105, and 4 × 10 nmol · L-1) could induce HMC oxidative stress injury, with significant decrease in the cell survival rate. AS IV (100 nmol · L-1) could significantly inhibit HMC oxidative stress injury induced by H2O2(3 × 10 nmol · L-1), increase the survival rate of HMC cells, inhibit cell apoptosis, and decrease intracellular ROS production. AS IV could also increase the expression of Cyclin D1, recover normal cell proliferation, and decrease the expression of p38. Conclusion: AS IV can protect H2O2induced oxidative stress injury in mesangial cells. Its mechanisms may be related to inhibiting the p38/MAPK signaling pathway, increasing the expression of Cyclin D1 and decreasing the intracellular ROS oxidative stress injury.

Jiang G.,Anhui University | Wu H.,Anhui University | Hu Y.,Anhui Medical University | Li J.,Anhui University | And 2 more authors.
Cellular and Molecular Neurobiology | Year: 2014

Previous research demonstrated that glutamate induces neuronal injury partially by increasing intracellular Ca2+ concentrations ([Ca 2+]i), and inducing oxidative stress, leading to a neurodegenerative disorder. However, the mechanism of glutamate-induced injury remains elusive. Gastrodin, a major active component of the traditional herbal agent Gastrodia elata (GE) Blume, has been recognized as a potential neuroprotective drug. In the current study, a classical injury model based on glutamate-induced cell death of rat pheochromocytoma (PC12) cells was used to investigate the neuroprotective effect of gastrodin, and its potential mechanisms involved. In this paper, the presence of gastrodin inhibits glutamate-induced oxidative stress as measured by the formation of reactive oxygen species (ROS), the level of malondialdehyde (MDA), mitochondrial membrane potential (MMP), and superoxide dismutase (SOD); gastrodin also prevents glutamate-induced [Ca2+]i influx, blocks the activation of the calmodulin-dependent kinase II (CaMKII) and the apoptosis signaling-regulating kinase-1 (ASK-1), inhibits phosphorylation of p38 mitogen-activated kinase (MAPK). Additionally, gastrodin blocked the expression of p53 phosphorylation, caspase-3 and cytochrome C, reduced bax/bcl-2 ratio induced by glutamate in PC12 cells. All these findings indicate that gastrodin protects PC12 cells from the apoptosis induced by glutamate through a new mechanism of the CaMKII/ASK-1/p38 MAPK/p53-signaling pathway. © 2014 Springer Science+Business Media New York.

Luan J.,Anhui Medical University | Li W.,Anhui Medical University | Li W.,Anqing Medical and Pharmaceutical College | Han J.,Anhui Medical University | And 4 more authors.
Journal of Pharmacological Sciences | Year: 2012

The present study was carried out to investigate the protective effects of tempol on renal function and the underlying mechanism in streptozotocin-induced diabetic rats. The diabetic rats were randomly divided into the model group (without tempol) and tempol group (1 mM tempol in drinking water for 6 weeks). Nondiabetic rats were served as the Control group. The mRNA expression of canonical transient receptor potential 6 (TRPC6), transforming growth factor (TGF)-β1, and type IV collagen (Col IV) were examined. The malondialdehyde (MDA) level, activities of superoxide dismutase (SOD), and glutathione peroxidase (GSH-Px) in renal tissues were measured to assess redox status in kidneys. We found that tempol significantly reduced 24-h urine output and urine albuminuria excretion in the diabetic rats. Compared with the model group, the concentration of MDA was significantly lower in the tempol group. In addition, diabetes decreased activities of SOD and GSH-Px and these responses were prevented by tempol treatment. Moreover, in diabetic rats, the mRNA expression levels of TGF-β1 and Col IV were upregulated. TRPC6 mRNA expression level was down-regulated in diabetic kidneys. However, all of these diabetic effects were significantly suppressed by tempol treatment. These results suggest that chronic treatment of diabetic rats with tempol can protect kidneys, possibly by reducing expression of TGF-β1, Col IV, and upregulating TRPC6 expression level. © The Japanese Pharmacological Society.

Zhang L.,Anhui University | Guo L.,Anhui University | Ding J.,Anqing Medical and Pharmaceutical College | Lu Y.,Anhui University | And 2 more authors.
Bulletin of the Korean Chemical Society | Year: 2015

In order to develop the application of the natural polysaccharide lentinan (LNT) and decrease the side effects of doxorubicin, we successfully synthesized a novel folate-modified maleilated lentinan-doxorubicin (FA-M. LNT-DOX) polymer and used it for tumor-targeted drug delivery. The release efficiency and cytotoxicity of the prodrugs were evaluated in vitro. Although DOX release from FA-M.LNT-DOX was quite slow in a neutral buffer, it was particularly fast in an acidic solution with a pH of 5.0. Compared with DOX, FA-M. LNT-DOX showed higher cytotoxicity in HeLa cells and significantly lower cytotoxicity in normal cells. These results suggested that FA-M.LNT-DOX could be considered as a potential drug delivery candidate for folate receptor-positive cancer therapy. © 2015 Korean Chemical Society & Wiley-VCH Verlag GmbH & Co. KGaA.

Ren L.-Y.,Anqing Medical and Pharmaceutical College | Zhang Q.,Anhui Normal University | Zhu W.-H.,Anhui Normal University | Li Q.-Q.,Anhui Normal University | Mao X.-M.,Anqing Medical and Pharmaceutical College
Rengong Jingti Xuebao/Journal of Synthetic Crystals | Year: 2015

Vaterite microspheres and microrings were fabricated by the vapor diffusion method. The effect of PSS and L-Lys on the morphology of CaCO3 particles were studied. The obtained products were characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM) and Fourier transform infrared (FT-IR) spectroscopy. The results reveal that, in the presence of PSS or L-Lys, the phase formed is vaterite spheres; in the presence of PSS and L-Lys, the microrings appear in the products. With increasing the concentration of PSS or L-Lys, the mount of microrings increases. Moreover, the possible formation mechanism of the hollow spherical structure was proposed. ©, 2015, Chinese Ceramic Society. All right reserved.

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