Anqing Medical and Pharmaceutical College

Anqing, China

Anqing Medical and Pharmaceutical College

Anqing, China

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Wu P.,Anhui Medical University | Wang J.-Y.,Anhui Medical University | Zhang X.-D.,Anhui Medical University | Zhu X.-F.,Sun Yat Sen University | And 2 more authors.
Tumor | Year: 2013

Objective: To investigate the promoting effect of TM (tunicamycin) on apoptosis of gastric cancer cells induced by TRAIL [TNF (tumor necrosis factor)-related apoptosis-inducing ligand], and to explore its possible mechanism. Methods: The effect of treatment with TM (1 μmol/L)/TRAIL (100 μg/L) alone or in combination for 3, 6, 16, 24 and 36 h on the apoptotic rate of SGC-7901 cells was detected by FCM (flow cytometry) using propidium iodide DNA staining. The cell surface expression levels of different types of TRAIL receptors including TRAIL-R1, TRAIL-R2, TRAIL-R3 and TRAIL-R4 before and after TM treatment were detected by FCM, and the expression level of TRAIL-R2 mRNA was detected by RFQ-PCR (real-time fluoregenic quantitative-PCR). The expression levels of GRP78 (78-kDa glucoseregulated protein) and CHOP (CCAAT/enhancer- binding protein homologous protein) proteins were detected by Western blotting. The splicing of XBP1 (X-box binding protein 1) mRNA was detected by RT-PCR. Results: Treatment with TM alone induced minimal level of apoptosis of SGC-7901 cells. The apoptosis rate of SGC-7901 cells was increased significantly after treatment with TM in combination with TRAIL. TM could markedly up-regulate cell surface expression level of TRAIL-R2 on cell surface. In contrast, TM did not induce any changes in the expressions of TRAIL-R1, TRAIL-R3 and TRAIL-R4. The expression level of TRAIL-R2 mRNA of SGC-7901 cells induced by TM treatment was up-regulated in a time-independent manner. The results of up-regulation of GRP78 and the splicing of XBP1 mRNA demonstrated the activation of UPR (unfolded protein response) induced by TM. Treatment with TM also resulted in a remarkable increase in the expression of CHOP protein. Conclusion: TM enhances TRAILinduced apoptosis in gastric cancer cells by up-regulation of TRAIL-R2 expression via UPR. CHOP may be responsible for this effect involved in up-regulation of TRAIL-R2. Copyright © 2013 by TUMOR.


Chen M.,Anhui University of Traditional Chinese Medicine | Chen M.,Anhui Medical University | Chen Z.-W.,Anhui Medical University | Long Z.-J.,Anhui University of Traditional Chinese Medicine | And 3 more authors.
Zhongguo Zhongyao Zazhi | Year: 2013

Objective: To investigate the effects of sapindus saponins on myocardial inflammation mediated by Ang II/p38MAPK signal pathway and cardiac hypertrophy in spontaneously hypertensive rats . And also to explore the correlation of cardiac hypertrophy and inflammation. Method: Thirty-two 16-week-old spontaneously hypertensive rats (SHR) were randomly divided into four groups, one with placebo as model group, one with captopril tablets(27 mg·kg-1)as positive control, one with low-dose sapindus saponins(27 mg·kg-1), one with high-dose (108 mg·kg-1). And another eight healthy Wistar-Kyoto strain(WKY) rats were used as the normal group. The animals were treated for eight weeks, and the indicators detected were as follows: (1)left ventricular mass index (LVMI); (2)the content of AngII and hs-CRP in plasma were determined by ELISA; (3)the protein expression of AT1R and VEGF were determined by immunohistochemical method; (4)the protein expression of p-p38MAPK in myocardial cells was determined by Western blot. Result: Sapindus saponins reduced LVMI, and blocked the expression level of AngII, AT1R, p-p38MAPK, VEGF and hs-CRP in myocardial tissue. Vs the SHR model group, there were significant differences (P<0.05 or P<0.01). Conclusion: Our findings suggested that sapindus saponins could inhibited cardiac hypertrophy, the possible mechanisms may be related to the inhibition on inflammatory response mediated by AngII/p38MAPK pathway.


Chen M.,Anhui Medical University | Chen M.,Anhui University of Traditional Chinese Medicine | Chen Z.-W.,Anhui Medical University | Long Z.-J.,Anhui University of Traditional Chinese Medicine | And 3 more authors.
Chinese Journal of Integrative Medicine | Year: 2015

Methods: Thirty-two 16-week-old spontaneously hypertensive rats were randomly divided into four groups (8 in each group): model group (placebo), positive control group (27 mg/kg of Captopril Tablets), Sapindus saponins groups (27 mg/kg and 108 mg/kg, respectively). Another 8 healthy Wistar-Kyoto strain (WKY) rats were used as the normal group. The animals were treated for 8 weeks. Blood pressure of rats was determined by non-invasive blood pressure meter (BP-6). Furthermore, the contents of angiotensin II (Ang II) in plasma and myocardial tissue were determined by enzyme-linked immunosorbent assay (ELISA), the gene expression of receptor angiotensin type 1 (AT1R) in aorta was determined by quantitative realtime polymerase chain reaction (qRT-PCR). The protein expression of transforming growth factor-β1 (TGF-β1) and AT1R in heart was determined by immunohistochemical staining. The protein expression of p-phosphorylation of p38 mitogen-activated protein kinase (p-p38MAPK) was determined by Western blotting. The contents of interleukin (IL)-1, IL-6 and tumor necrosis factor (TNF) in serum were determined by radioimmunoassay. And the histopathological and morphological changes of aorta and heart tissue samples were assessed semi-quantitatively by hematoxylin-eosin (HE) or Masson staining.Objectives: To investigate the protective effects of Sapindus saponins in spontaneously hypertensive rats, and the possible cellular and molecular mechanisms.Results: Thirty minutes after single or continuous treatment, systolic blood pressure (SBP) was reduced significantly in Sapindus saponins groups. And the contents of AngII, IL-1, IL-6 and TNF-α in serum, the expression of AT1R mRNA, p-p38MAPK and TGF-β1 were significantly suppressed dose-dependently (P<0.05 or P<0.01). With the Sapindus saponins treatment, compared with those of the model group, the cardiac and aortic pathological changes were ameliorated significantly.Conclusions: Our findings suggest that Sapindus saponins might have protective effects in spontaneously hypertensive rats, the cellular and molecular mechanisms of which might be relevant to the regulation of inflammatory responses mediated by p-p38MAPK signal pathway based on activated Ang II and AT1R. © 2013, Chinese Association of the Integration of Traditional and Western Medicine and Springer-Verlag Berlin Heidelberg.


Jiang G.,Anhui University | Hu Y.,Anhui Medical University | Hu Y.,No 105 Hospital Of Pla | Liu L.,No 174 Hospital Of Pla | And 4 more authors.
Neurochemistry International | Year: 2014

Although the etiology of PD remains unclear, increasing evidence has shown that oxidative stress plays an important role in its pathogenesis and that of other neurodegenerative disorders. The phenolic glucoside gastrodin, a main constituent of a Chinese herbal medicine Gastrodia elata (GE) Blume, has been known to display antioxidant activity. The present study aimed to investigate the protective effects of gastrodin on 1-methyl-4-phenylpyridinium (MPP +)-induced oxidative cytotoxicity in human dopaminergic SH-SY5Y cells and the underlying mechanism for this neuroprotection. Results indicate that pre-treatment with gastrodin for 1 h significantly reduced the MPP +-induced viability loss, apoptotic rate and attenuated MPP +-mediated ROS production. In addition, gastrodin inhibited MPP +-induced lowered membrane potential, decreased Bcl-2/Bax ratio. Moreover, we have revealed the gastrodin increased Nrf2 nuclear translocation, which is upstream of heme oxygenase-1 (HO-1) expression and for the first time revealed gastrodin could increased antioxidant enzyme HO-1 expression in concentration-dependent and time-dependent manners. HO-1 siRNA transfection was employed, and confirmed gastrodin could active the expression of HO-1. And the increase in HO-1 expression was correlated with the protective effect of gastrodin against MPP+-induced injury. Because the inhibitor of HO-1 activity, ZnPP reversed the protective effect of gastrodin against MPP +-induced cell death. We also demonstrated that the specific p38 MAPK inhibitor, SB203580, concentration-dependently blocked on gastrodin-induced HO-1 expression, and meanwhile SB203580 reversed the protective effect of gastrodin against MPP+-induced cell death. Taken together, these findings suggest that gastrodin can induce HO-1 expression through activation of p38 MAPK/Nrf2 signaling pathway, thereby protecting the SH-SY5Y cells from MPP+-induced oxidative cell death. Thus our study indicates that gastrodin has a partial cytoprotective role in dopaminergic cell culture systems and could be of importance for the treatment of PD and other oxidative stress-related diseases. © 2014 Elsevier Ltd. All rights reserved.


Wang M.,Anqing Medical and Pharmaceutical College | Zhao K.,Anqing Teachers College | Wang R.,Anqing Medical and Pharmaceutical College
Zhongguo Zhongyao Zazhi | Year: 2012

Through studies on morphological characteristics, distribution and ecological habitat described in ancient literatures, Osmunda japonica is believed to the original plant of Chinese herbal medicine Cyrtomium Rhizome. Meanwhile, analysis is also made on causes for descriptions that do not comply with characteristics of O. japonica such as toxicity, flowers and fruits, illustrations, indumentums and flakes as well as appearance of other original plants. It is suggested to list O. japonica as the only original plant of Cyrtomium Rhizome and distinguish it from other frequently seen medical plants. Separated studies are also conducted for pesticide effect and active ingredients of Dryoteris Crassrhizomae Rhizome and Woodwardia japonica that show better effects.


Cao L.-L.,Anhui Medical University | Cao L.-L.,Huainan Union University | Li W.-Z.,Anhui Medical University | Si X.-L.,Anhui Medical University | And 3 more authors.
Zhongguo Zhongyao Zazhi | Year: 2013

Objective: To study the protective effect of astragaloside IV (AS IV) on H2O2 induced human mesangial cells (HMC), and further explore its molecular mechanism. Method: The cultured mesangial cells were divided into 5 groups: the normal control group, the H2O2 model group, the AS IV (12.5, 100 nmol · L-1) group and the Tempol (1 × 105 nmol · L-1) group. The MTT method was used to observe cell viability. Hoechst 33258 staining was used to observe the HMC apoptosis and DHE staining was used to detect the generation of reactive oxygen species (ROS). The flow cytometry was used to detect the changes in cell cycle. Western blot was used to detect the expression of Cyclin D1, CyclinA, p38, and T-p38. Result: H2O2 (1 × 105, 2 × 105, 3 × 105, and 4 × 10 nmol · L-1) could induce HMC oxidative stress injury, with significant decrease in the cell survival rate. AS IV (100 nmol · L-1) could significantly inhibit HMC oxidative stress injury induced by H2O2(3 × 10 nmol · L-1), increase the survival rate of HMC cells, inhibit cell apoptosis, and decrease intracellular ROS production. AS IV could also increase the expression of Cyclin D1, recover normal cell proliferation, and decrease the expression of p38. Conclusion: AS IV can protect H2O2induced oxidative stress injury in mesangial cells. Its mechanisms may be related to inhibiting the p38/MAPK signaling pathway, increasing the expression of Cyclin D1 and decreasing the intracellular ROS oxidative stress injury.


Jiang G.,Anhui University | Wu H.,Anhui University | Hu Y.,Anhui Medical University | Li J.,Anhui University | And 2 more authors.
Cellular and Molecular Neurobiology | Year: 2014

Previous research demonstrated that glutamate induces neuronal injury partially by increasing intracellular Ca2+ concentrations ([Ca 2+]i), and inducing oxidative stress, leading to a neurodegenerative disorder. However, the mechanism of glutamate-induced injury remains elusive. Gastrodin, a major active component of the traditional herbal agent Gastrodia elata (GE) Blume, has been recognized as a potential neuroprotective drug. In the current study, a classical injury model based on glutamate-induced cell death of rat pheochromocytoma (PC12) cells was used to investigate the neuroprotective effect of gastrodin, and its potential mechanisms involved. In this paper, the presence of gastrodin inhibits glutamate-induced oxidative stress as measured by the formation of reactive oxygen species (ROS), the level of malondialdehyde (MDA), mitochondrial membrane potential (MMP), and superoxide dismutase (SOD); gastrodin also prevents glutamate-induced [Ca2+]i influx, blocks the activation of the calmodulin-dependent kinase II (CaMKII) and the apoptosis signaling-regulating kinase-1 (ASK-1), inhibits phosphorylation of p38 mitogen-activated kinase (MAPK). Additionally, gastrodin blocked the expression of p53 phosphorylation, caspase-3 and cytochrome C, reduced bax/bcl-2 ratio induced by glutamate in PC12 cells. All these findings indicate that gastrodin protects PC12 cells from the apoptosis induced by glutamate through a new mechanism of the CaMKII/ASK-1/p38 MAPK/p53-signaling pathway. © 2014 Springer Science+Business Media New York.


Luan J.,Anhui Medical University | Li W.,Anhui Medical University | Li W.,Anqing Medical and Pharmaceutical College | Han J.,Anhui Medical University | And 4 more authors.
Journal of Pharmacological Sciences | Year: 2012

The present study was carried out to investigate the protective effects of tempol on renal function and the underlying mechanism in streptozotocin-induced diabetic rats. The diabetic rats were randomly divided into the model group (without tempol) and tempol group (1 mM tempol in drinking water for 6 weeks). Nondiabetic rats were served as the Control group. The mRNA expression of canonical transient receptor potential 6 (TRPC6), transforming growth factor (TGF)-β1, and type IV collagen (Col IV) were examined. The malondialdehyde (MDA) level, activities of superoxide dismutase (SOD), and glutathione peroxidase (GSH-Px) in renal tissues were measured to assess redox status in kidneys. We found that tempol significantly reduced 24-h urine output and urine albuminuria excretion in the diabetic rats. Compared with the model group, the concentration of MDA was significantly lower in the tempol group. In addition, diabetes decreased activities of SOD and GSH-Px and these responses were prevented by tempol treatment. Moreover, in diabetic rats, the mRNA expression levels of TGF-β1 and Col IV were upregulated. TRPC6 mRNA expression level was down-regulated in diabetic kidneys. However, all of these diabetic effects were significantly suppressed by tempol treatment. These results suggest that chronic treatment of diabetic rats with tempol can protect kidneys, possibly by reducing expression of TGF-β1, Col IV, and upregulating TRPC6 expression level. © The Japanese Pharmacological Society.


Zhang L.,Anhui University | Guo L.,Anhui University | Ding J.,Anqing Medical and Pharmaceutical College | Lu Y.,Anhui University | And 2 more authors.
Bulletin of the Korean Chemical Society | Year: 2015

In order to develop the application of the natural polysaccharide lentinan (LNT) and decrease the side effects of doxorubicin, we successfully synthesized a novel folate-modified maleilated lentinan-doxorubicin (FA-M. LNT-DOX) polymer and used it for tumor-targeted drug delivery. The release efficiency and cytotoxicity of the prodrugs were evaluated in vitro. Although DOX release from FA-M.LNT-DOX was quite slow in a neutral buffer, it was particularly fast in an acidic solution with a pH of 5.0. Compared with DOX, FA-M. LNT-DOX showed higher cytotoxicity in HeLa cells and significantly lower cytotoxicity in normal cells. These results suggested that FA-M.LNT-DOX could be considered as a potential drug delivery candidate for folate receptor-positive cancer therapy. © 2015 Korean Chemical Society & Wiley-VCH Verlag GmbH & Co. KGaA.


Ren L.-Y.,Anqing Medical and Pharmaceutical College | Zhang Q.,Anhui Normal University | Zhu W.-H.,Anhui Normal University | Li Q.-Q.,Anhui Normal University | Mao X.-M.,Anqing Medical and Pharmaceutical College
Rengong Jingti Xuebao/Journal of Synthetic Crystals | Year: 2015

Vaterite microspheres and microrings were fabricated by the vapor diffusion method. The effect of PSS and L-Lys on the morphology of CaCO3 particles were studied. The obtained products were characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM) and Fourier transform infrared (FT-IR) spectroscopy. The results reveal that, in the presence of PSS or L-Lys, the phase formed is vaterite spheres; in the presence of PSS and L-Lys, the microrings appear in the products. With increasing the concentration of PSS or L-Lys, the mount of microrings increases. Moreover, the possible formation mechanism of the hollow spherical structure was proposed. ©, 2015, Chinese Ceramic Society. All right reserved.

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