Anna Bioresearch Foundation

Tiruvannāmalai, India

Anna Bioresearch Foundation

Tiruvannāmalai, India
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Palani S.,Anna Bioresearch Foundation | Raja S.,Bharat Institute of Technology | Kumar R.P.,Anna Bioresearch Foundation | Parameswaran P.,Anna Bioresearch Foundation | Kumar B.S.,PG Research
Acta Pharmaceutica Sciencia | Year: 2010

Acorus calamus (AC) is a traditional medicinal plant that is commonly used for disorder of central nervous system abnormalities. In ayurvedic medicine, it is used for the treatment of insomnia, melancholia, epilepsy, hysteria, loss of memory remittent fevers and neurosis. This plant extract is mainly used for various pharmacological activities like antidiabetic, antiproliferative, immunosuppressive, antidiarrhoeal and hypolipidemic activities. The main constituents of AC were found belonging to monoterpene, sesquiterpene, phenylpropanoid, flavonoid and quinone. The aim of this study was to investigate the nephroprotective and antioxidant activities of ethanol extract of AC at two dose levels of 250 and 500 mg/kg B/W on acetaminophen (APAP) induced toxicity in male albino rats. APAP significantly increased levels of serum urea, hemoglobin (Hb), total leukocyte count, packed cell volume, creatinine, DLC, and mean corpuscular volume, raised body weight, and reduced levels of neutrophils, mean corpuscular Hb content, mean corpuscular hematocrit, granulocytes, uric acid, and platelet Concentration. AC inhibited the hematological effects of APAP. AC significantly increased activities of renal superoxide dismutase, catalase, glutathione, and glutathione peroxidase and decreased malondialdehyde content of APAP-treated rats. Apart from these, histopathological changes also showed the protective nature of the AC extract against APAPinduced necrotic damage of renal tissues. In conclusion it was observed that the ethanol extract of AC conferred nephroprotective and antioxidant activities by histopathological and biochemical observations against APAP induced renal damage in rats.


Palani S.,Anna Bioresearch Foundation | Palani S.,PG Research | Raja S.,Bharat Institute of Technology | Senthil Kumar B.,PG Research
International Journal of PharmTech Research | Year: 2010

Chloroxylon swietenia is a folklore medicinal plant that is commonly used for antimicrobial, antifertility, analgesic, insecticidal, antifeedant activities The present study was aimed to investigate the hepatoprotective and antioxidant activities of ethanol extract of Chloroxylon swietenia (250mg/kg & 500 mg/kg B/W) on acetaminophen (APAP) induced rat hepatic injury. The development of hepatotoxicity induced by APAP is promoted by oxidative stress. APAP treated group significantly (p<0.01) elevated the serum enzymatic levels like serum glutamate pyruvate transaminase (ALT), serum glutamate oxaloacetate transaminase (AST), serum alkaline phosphatase (SALP) and total bilirubin, which were restored towards normalization significantly (p<0.01) by the ethanol extract of Chloroxylon swietenia in a dose dependent manner. In addition, this plant extract significantly (p<0.01) elevated the decreased level of antioxidant enzymes such as glutathione peroxidase (GPX), catalase (CAT), glutathione-s-transferase (GST) superoxide dismutase (SOD) and reduced glutathione (GSH). Biochemical and Histopathological observations confirmed the beneficial roles of ethanol extract of Chloroxylon swietenia against acetaminophen induced liver injury in rats. Histological analysis of the liver of these rats revealed marked necro-inflammatory changes by APAP and ethanol extract of Chloroxylon swietenia attenuated the necro-inflammatory changes in the liver. The activity of ethanol extract of Chloroxylon swietenia (500 mg/kg B/W) was comparable to the standard drug silymarin (25mg/kg B/W).


Palani S.,Anna Bioresearch Foundation | Raja S.,Bharat Institute of Technology | Kumar R.P.,Anna Bioresearch Foundation | Selvaraj R.,Anna Bioresearch Foundation | Kumar B.S.,PG Research
Pakistan Journal of Pharmaceutical Sciences | Year: 2011

Monochoria vaginalis is an herbaceous medicinal plant used to treat, liver problems India. Acetaminophen is a commonly used analgesic and antipyretic agent which, at high doses, causes liver and kidney necrosis in man and animals. The aim of the present study is to evaluate phytoconstituents and investigate the nephroprotective & antioxidant activities of the ethanol extract of Monochoria vaginalis on acetaminophen induced toxicity in rats. Phytoconstituents like n-hexadecanoic acid, 3-methyl- acetate-1-butanol, 1,1,3-triethoxy- propane, Z,Z,Z-1,4,6,9 - nonadecatetraene, undecanoic acid, 3-trifluoroacetoxy penta decane and 4-ethyl-5-octyl-2,2-bis (trifluoromethyl) - cis-1,3-dioxalone were identified from ethanol extract of Monochoria vaginalis by using a gas chromatograph-mass spectrograph (GC MS). Biochemical studies show that there is an increase in the levels of serum urea and creatinine along with an increase in the body weight and reduction in the levels of uric acid in acetaminophen induced groups. These values are retrieved significantly by treatment with Monochoria vaginalis extracts at two different doses. The antioxidant studies reveal that the levels of renal SOD, CAT, GSH and GPx in the APAP treated animals are increased significantly along with a reduced MDA content in ethanol extract of Monochoria vaginalis treated groups. Apart from these, histopathological changes also reveal the protective nature of the Monochoria vaginalis extract against acetaminophen induced necrotic damage of renal tissues. In conclusion, these data suggest that the ethanol extract of Monochoria vaginalis can prevent renal damage from APAP induced nephrotoxicity in rats and it is likely to be mediated through active phytoconstituents and its antioxidant activities.


Padmalochana K.,Bharathiar University | Padmalochana K.,Sri Akilandeswari Womens College | Sivasankari H.,Anna Bioresearch foundation
Journal of Applied Pharmaceutical Science | Year: 2013

Cryptolepis buchanani (Berberidaceae) is a climbing tree, leaves is widely used in folk medicine in Southeast Asia. The alcoholic extract of stem of this plant is commonly used for the treatment of inflammatory conditions such as arthritis, and muscle and joint pain. The development of hepatotoxicity induced by acetaminophen is promoted by oxidative stress. The aim of the present study was to investigate the hepatoprotective effect and antioxidant activities ofthe ethanolic extract of Cryptolepis buchanani on acetaminophen induced hepatotoxicity in rats. We observed that the ethanolic extract of cryptolepis buchanani conferred hepatoprotectivity. Biochemical observations confirmed the beneficial roles of Cryptolepis buchanani and silymarin against acetaminophen induced liver injury in rats.


Balasubramaniam B.,Anna Bioresearch Foundation | Sudalaiyadum Perumal A.,Anna Bioresearch Foundation | Jayaraman J.,Anna University | Ramanujam P.,Anna Bioresearch Foundation
Waste Management | Year: 2012

The petroleum fuel is nearing the line of extinction. Recent research and technology have provided promising outcomes to rely on biodiesel as the alternative and conventional source of fuel. The use of renewable source - vegetable oil constitutes the main stream of research. In this preliminary study, Waste Cooking Oil (WCO) was used as the substrate for biodiesel production. Lipase enzyme producing fungi Rhizopus oryzae 262 and commercially available pure lipase enzyme were used for comparative study in the production of Fatty Acid Alkyl Esters (FAAE). The whole cell (RO 262) and pure lipase enzyme (PE) were immobilized using calcium alginate beads. Calcium alginate was prepared by optimizing with different molar ratios of calcium chloride and different per cent sodium alginate. Entrapment immobilization was done for whole cell biocatalyst (WCB). PE was also immobilized by entrapment for the transesterification reaction. Seven different solvents - methanol, ethanol, n-propanol, n-butanol, iso-propanol, iso-butanol and iso-amyl alcohol were used as the acyl acceptors. The reaction parameters like temperature (30°C), molar ratio (1:3 - oil:solvent), reaction time (24. h), and amount of enzyme (10% mass ratio to oil) were also optimized for methanol alone. The same parameters were adopted for the other acyl acceptors too. Among the different acyl acceptors - methanol, whose reaction parameters were optimized showed maximum conversion of triglycerides to FAAE-94% with PE and 84% with WCB. On the whole, PE showed better catalytic converting ability with all the acyl acceptor compared to WCB. Gas chromatography analysis (GC) was done to determine the fatty acid composition of WCO (sunflower oil) and FAAE production with different acyl acceptors. © 2012 Elsevier Ltd.


Vijay T.,Bharathiar University | Vijay T.,P.A. College | Rajan M.S.D.,P.A. College | Sarumathy K.,Bharathiar University | And 3 more authors.
Journal of Applied Pharmaceutical Science | Year: 2011

Doxorubicin (DOX) is a widely used cancer chemotherapeutic agent. However, it generates free oxygen radicals that result in serious dose-limiting cardiotoxicity. Supplementations with Gmelina arborea (Verbenaceae) were proven effective in reducing oxidative stress associated with several ailments. The aim of the current study was to investigate the potential protective effect of Gmelina arborea (GA) against DOX- induced cardiotoxicity in rats. GA was given orally to rats(250&500mg/kg) and DOX (20mg/kg) was administered on the seventh day. GA protected against DOX-induced increased the levels of marker enzymes. It significantly inhibited DOX-provoked glutathione (GSH) depletion in cardiac tissues. The reductions of cardiac activities of catalase (CAT) ,superoxide dismutase (SOD) ,glutathione peroxidase (GSH-Px) and glutathione reductase (GR) were significantly mitigated. Pretreatment of GA significantly guarded against DOX-induced rise of serum lactate dehydrogenase (LDH). GA alleviated histopathological changes in rats' hearts treated with DOX. In conclusion, GA protects against DOX-induced cardiotoxicity in rats. The study can be attributed, at least in part, to GA's antioxidant activity.


Gomathy G.,Valliammal College for Women | Gomathy G.,Tamil University | Venkatesan D.,Arunai Engineering College | Palani S.,Anna Bioresearch Foundation
Natural Product Research | Year: 2015

This study investigated the protective effects of the ethanolic extract of Mukia maderaspatana against indomethacin-induced gastric ulcer in rats. Gastric ulceration was induced by single intraperitoneal injection of indomethacin (30 mg/kg b.wt.). M. maderaspatana extract produced significant reduction in gastric mucosal lesions, malondialdehyde and serum tumour necrosis factor-α associated with a significant increase in gastric juice mucin content and gastric mucosal catalase, nitric oxide and prostaglandin E2 levels. The volume and acidity of the gastric juice decreased in pretreated rats. The plant extract was evaluated in the gastric juice of rats, untreated has showed near normal levels in pretreated rats. The M. maderaspatana was able to decrease acidity and increase the mucosal defence in the gastric area, therefore justifying its use as an antiulcerogenic agent. Ranitidine significantly increased pH value and decreased pepsin activity and gastric juice free and total acidity. The anti-ulcer effect was further confirmed histologically. © 2014 Taylor & Francis.


Palani S.,Anna Bioresearch Foundation | Raja S.,Bharat Institute of Technology | Kalash R.S.,Anna Bioresearch Foundation | Kumar B.S.,PG Research
Toxicological and Environmental Chemistry | Year: 2010

Mahonia leschenaultia takeda (Berberidaceae) (MLT) is a traditional medicinal plant that is commonly used to treat and improve liver and renal conditions in India and other Asian countries. Acetaminophen (APAP) is commonly used as an analgesic and an antipyretic agent that, in high doses, produces liver and kidney necrosis in mammals. The aim of this study was to investigate the nephroprotective and antioxidant activities of the ethanol extract of MLT at two dose levels of 250 and 500 mg kg-1 bw on APAP-induced toxicity in rats. APAP significantly increased levels of serum urea, hemoglobin (Hb), total leukocyte count, creatinine, packed cell volume, DLC, and mean corpuscular volume, raised body weight, and reduced levels of neutrophils, mean corpuscular Hb content, mean corpuscular hematocrit, granulocytes, uric acid, and platelet concentration. MLT inhibited the hematological effects of APAP. MLT significantly increased activities of renal superoxide dismutase, catalase, glutathione, and glutathione peroxidase and decreased malondialdehyde content of APAP-treated rats. Apart from these, histopathological changes also showed the protective nature of the MLT extract against APAP-induced necrotic damage of renal tissues. In conclusion, data suggest that the ethanol extract of MLT prevented renal damage from APAP-induced nephrotoxicity in rats and it is likely mediated through antioxidant activities. © 2010 Taylor & Francis.


Palani S.,Anna Bioresearch Foundation | Palani S.,PG Research | Raja S.,Bharat Institute of Technology | Naresh R.,Anna Bioresearch Foundation | Kumar B.S.,PG Research
Toxicology Mechanisms and Methods | Year: 2010

Plectranthus amboinicus (PA), commonly known as country borage, is a folkoric medicinal plant. Juice from its leaves is commonly used for illnesses including liver and renal conditions in the Asian sub-continent. Acetaminophen (APAP), used as an analgesic, produces liver and kidney necrosis in mammals at high doses. The aim of this study was to investigate the nephroprotective, diuretic, and antioxidant activities of the ethanol extract of PA at two doses of 250 and 500 mg/kg bw on APAP-induced toxicity in rats. This study shows that APAP significantly increases the levels of serum urea (UR), hemoglobin (Hb), total leukocyte count, creatinine, raised body weight, and reduced levels of neutrophils, granulocytes, uric acid, and platelet concentration. Ethanol extract of PA rescued these phenotypes by increasing anti-oxidative responses as assessed by biochemistry and histopathology. In addition, the ethanol extract of PA at two doses showed a significant diuretic activity by increased levels of total urine output and urinary elerolytes such as sodium and potassium. In conclusion, these data suggest that the ethanol extract of PA possess nephroprotective and antioxidant effects against APAP-induced nephrotoxicity and strong diuretics effect in rats. © 2010 Informa UK Ltd.


PubMed | Anna Bioresearch Foundation
Type: Comparative Study | Journal: Waste management (New York, N.Y.) | Year: 2012

The petroleum fuel is nearing the line of extinction. Recent research and technology have provided promising outcomes to rely on biodiesel as the alternative and conventional source of fuel. The use of renewable source - vegetable oil constitutes the main stream of research. In this preliminary study, Waste Cooking Oil (WCO) was used as the substrate for biodiesel production. Lipase enzyme producing fungi Rhizopus oryzae 262 and commercially available pure lipase enzyme were used for comparative study in the production of Fatty Acid Alkyl Esters (FAAE). The whole cell (RO 262) and pure lipase enzyme (PE) were immobilized using calcium alginate beads. Calcium alginate was prepared by optimizing with different molar ratios of calcium chloride and different per cent sodium alginate. Entrapment immobilization was done for whole cell biocatalyst (WCB). PE was also immobilized by entrapment for the transesterification reaction. Seven different solvents - methanol, ethanol, n-propanol, n-butanol, iso-propanol, iso-butanol and iso-amyl alcohol were used as the acyl acceptors. The reaction parameters like temperature (30C), molar ratio (1:3 - oil:solvent), reaction time (24 h), and amount of enzyme (10% mass ratio to oil) were also optimized for methanol alone. The same parameters were adopted for the other acyl acceptors too. Among the different acyl acceptors - methanol, whose reaction parameters were optimized showed maximum conversion of triglycerides to FAAE-94% with PE and 84% with WCB. On the whole, PE showed better catalytic converting ability with all the acyl acceptor compared to WCB. Gas chromatography analysis (GC) was done to determine the fatty acid composition of WCO (sunflower oil) and FAAE production with different acyl acceptors.

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