Anna Bioresearch Foundation

Tiruvannāmalai, India

Anna Bioresearch Foundation

Tiruvannāmalai, India

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Padmalochana K.,Bharathiar University | Padmalochana K.,Sri Akilandeswari Womens College | Sivasankari H.,Anna Bioresearch foundation
Journal of Applied Pharmaceutical Science | Year: 2013

Cryptolepis buchanani (Berberidaceae) is a climbing tree, leaves is widely used in folk medicine in Southeast Asia. The alcoholic extract of stem of this plant is commonly used for the treatment of inflammatory conditions such as arthritis, and muscle and joint pain. The development of hepatotoxicity induced by acetaminophen is promoted by oxidative stress. The aim of the present study was to investigate the hepatoprotective effect and antioxidant activities ofthe ethanolic extract of Cryptolepis buchanani on acetaminophen induced hepatotoxicity in rats. We observed that the ethanolic extract of cryptolepis buchanani conferred hepatoprotectivity. Biochemical observations confirmed the beneficial roles of Cryptolepis buchanani and silymarin against acetaminophen induced liver injury in rats.


Balasubramaniam B.,Anna Bioresearch Foundation | Sudalaiyadum Perumal A.,Anna Bioresearch Foundation | Jayaraman J.,Anna University | Ramanujam P.,Anna Bioresearch Foundation
Waste Management | Year: 2012

The petroleum fuel is nearing the line of extinction. Recent research and technology have provided promising outcomes to rely on biodiesel as the alternative and conventional source of fuel. The use of renewable source - vegetable oil constitutes the main stream of research. In this preliminary study, Waste Cooking Oil (WCO) was used as the substrate for biodiesel production. Lipase enzyme producing fungi Rhizopus oryzae 262 and commercially available pure lipase enzyme were used for comparative study in the production of Fatty Acid Alkyl Esters (FAAE). The whole cell (RO 262) and pure lipase enzyme (PE) were immobilized using calcium alginate beads. Calcium alginate was prepared by optimizing with different molar ratios of calcium chloride and different per cent sodium alginate. Entrapment immobilization was done for whole cell biocatalyst (WCB). PE was also immobilized by entrapment for the transesterification reaction. Seven different solvents - methanol, ethanol, n-propanol, n-butanol, iso-propanol, iso-butanol and iso-amyl alcohol were used as the acyl acceptors. The reaction parameters like temperature (30°C), molar ratio (1:3 - oil:solvent), reaction time (24. h), and amount of enzyme (10% mass ratio to oil) were also optimized for methanol alone. The same parameters were adopted for the other acyl acceptors too. Among the different acyl acceptors - methanol, whose reaction parameters were optimized showed maximum conversion of triglycerides to FAAE-94% with PE and 84% with WCB. On the whole, PE showed better catalytic converting ability with all the acyl acceptor compared to WCB. Gas chromatography analysis (GC) was done to determine the fatty acid composition of WCO (sunflower oil) and FAAE production with different acyl acceptors. © 2012 Elsevier Ltd.


Selvaraj R.,Anna Bioresearch Foundation | Bharathiraja B.,Dr Rangarajan And Dr Sakunthala Engineering Colleges | Palani S.,Anna Bioresearch Foundation | Praveenkumar R.,Anna Bioresearch Foundation
Acta Horticulturae | Year: 2014

Bacteriocin, antibacterial peptides, are regarded as potential alternatives to conventional antimicrobials from probiotics tested against different test organisms. They are a family of microbial defense systems which may inhibit the invasion of strains and act as biopreservatives. Antimicrobial activity of crude bacteriocin was assessed and characterized by the Agar Well Diffusion Assay and the cell-free extract was collected by centrifugation of broths, poured into the wells and tested against test organisms. The activity of the culture filtrate was measured by the zone of inhibition. Statistical screening of variables for enhancement of the production of biopreservatives was carried out. Bacteriocin was effective against E. coli and Staphylococcus from the analysis; dextrose, sodium acetate, ammonium citrate and NaCl were screened as important variables using the Placket-Burman Design. Low-cost nitrogen sources often used in production were used to replace their expensive counterparts in De Man Rogosa and Sharpe (MRS) constituents were used in lower concentration than in the MRS medium for control. Three variables were chosen from the seven variables considered in the Placket-Burman (PB) design. Response surface method was used to study and optimize their interaction and to determine their optimimum levels. Central composite design (CCD) was used to move toward the optimum response and determined the maximum activity of the bacteriocin. By solving the regression equation and analyzing the response surface, the optimal concentrations of dextrose (15 g/L), sodium acetate (4.5 g/L), ammonium citrate (2.5 g/L) and NaCl (1.5 g/L) stimulated the production of bacteriocin and the optimal equation was then verified. Under optimized conditions, microbial growth had two-fold higher production than common MRS. The molecular mass of purified bacteriocin was approximately 2kD by SDS-PAGE. All experiments were done at room temperature for two days under stationary condition. The protein extraction was completed by dialysis, followed by precipitation, purification by ammonium sulphate precipitation and then chromatography. The new medium was less expensive. Therefore, it has more significance for application as a biopreservative in food products. The improvement in production was achieved due to this optimized medium. The results indicated the importance of statistical tools for the enhanced production of bacteriocin. The potential of preservation could be achieved either by a bacteriocin starter culture or by bacteriocin as a food additive. © 2014, International Society for Horticultural Science. All rights reserved.


Praveenkumar R.,Anna Bioresearch Foundation | Suresh K.,Anna Bioresearch Foundation | Chozhavendhan S.,Anna Bioresearch Foundation | Bharathiraja B.,HIGH-TECH
International Journal of ChemTech Research | Year: 2014

Among the various environmental issues, waste management is a foremost area where effectual and exhaustive consumption of wastes and attaining useful products in natal ways are concentrated. Cassava the tuber crop, exploited for starch and sago production.The aim of this work is management of cassava sago industrial waste, because its availability is vast particularly in salem. Bio ethanol- high octane fuel that produced from renewed sources such as sugary (or) starchy agricultural crops. As a point of interest, it was affirm the disposed waste consists 55-60% of starch by biochemical characterization. On degradation, starch breaks up into simpler sugar units that simple sugars consumed for ethanol production by action of fermentation through analyzing various parameters, showing the excellent efficiency of the method is isolation of native starch degrading microorganism, instead of using enzymes for starch simplification. The starch simplifying organisms were isolated from the sago industry waste(Bacillus sp)as well as isolated frompotato(Aspergillus niger) and fruitfully established into the fermentation as separately in batch fermentation by co-cultivation. It was further accompanied by ethanol production using Saccharomyces cereviseae. The highest product yield was attained in fungal saccharification of sago waste which was pretreated by acid pretreatment method. The maximum ethanol production was 3.02 g/L from 5 g/L of initial sago waste concentration. © 2014, Sphinx Knowledge House. All rights Reserved.


Vijay T.,Bharathiar University | Vijay T.,P.A. College | Rajan M.S.D.,P.A. College | Sarumathy K.,Bharathiar University | And 3 more authors.
Journal of Applied Pharmaceutical Science | Year: 2011

Doxorubicin (DOX) is a widely used cancer chemotherapeutic agent. However, it generates free oxygen radicals that result in serious dose-limiting cardiotoxicity. Supplementations with Gmelina arborea (Verbenaceae) were proven effective in reducing oxidative stress associated with several ailments. The aim of the current study was to investigate the potential protective effect of Gmelina arborea (GA) against DOX- induced cardiotoxicity in rats. GA was given orally to rats(250&500mg/kg) and DOX (20mg/kg) was administered on the seventh day. GA protected against DOX-induced increased the levels of marker enzymes. It significantly inhibited DOX-provoked glutathione (GSH) depletion in cardiac tissues. The reductions of cardiac activities of catalase (CAT) ,superoxide dismutase (SOD) ,glutathione peroxidase (GSH-Px) and glutathione reductase (GR) were significantly mitigated. Pretreatment of GA significantly guarded against DOX-induced rise of serum lactate dehydrogenase (LDH). GA alleviated histopathological changes in rats' hearts treated with DOX. In conclusion, GA protects against DOX-induced cardiotoxicity in rats. The study can be attributed, at least in part, to GA's antioxidant activity.


Gomathy G.,Valliammal College for Women | Gomathy G.,Tamil University | Venkatesan D.,Arunai Engineering College | Palani S.,Anna Bioresearch Foundation
Natural Product Research | Year: 2015

This study investigated the protective effects of the ethanolic extract of Mukia maderaspatana against indomethacin-induced gastric ulcer in rats. Gastric ulceration was induced by single intraperitoneal injection of indomethacin (30 mg/kg b.wt.). M. maderaspatana extract produced significant reduction in gastric mucosal lesions, malondialdehyde and serum tumour necrosis factor-α associated with a significant increase in gastric juice mucin content and gastric mucosal catalase, nitric oxide and prostaglandin E2 levels. The volume and acidity of the gastric juice decreased in pretreated rats. The plant extract was evaluated in the gastric juice of rats, untreated has showed near normal levels in pretreated rats. The M. maderaspatana was able to decrease acidity and increase the mucosal defence in the gastric area, therefore justifying its use as an antiulcerogenic agent. Ranitidine significantly increased pH value and decreased pepsin activity and gastric juice free and total acidity. The anti-ulcer effect was further confirmed histologically. © 2014 Taylor & Francis.


Palani S.,Anna Bioresearch Foundation | Raja S.,Bharat Institute of Technology | Kalash R.S.,Anna Bioresearch Foundation | Kumar B.S.,PG Research
Toxicological and Environmental Chemistry | Year: 2010

Mahonia leschenaultia takeda (Berberidaceae) (MLT) is a traditional medicinal plant that is commonly used to treat and improve liver and renal conditions in India and other Asian countries. Acetaminophen (APAP) is commonly used as an analgesic and an antipyretic agent that, in high doses, produces liver and kidney necrosis in mammals. The aim of this study was to investigate the nephroprotective and antioxidant activities of the ethanol extract of MLT at two dose levels of 250 and 500 mg kg-1 bw on APAP-induced toxicity in rats. APAP significantly increased levels of serum urea, hemoglobin (Hb), total leukocyte count, creatinine, packed cell volume, DLC, and mean corpuscular volume, raised body weight, and reduced levels of neutrophils, mean corpuscular Hb content, mean corpuscular hematocrit, granulocytes, uric acid, and platelet concentration. MLT inhibited the hematological effects of APAP. MLT significantly increased activities of renal superoxide dismutase, catalase, glutathione, and glutathione peroxidase and decreased malondialdehyde content of APAP-treated rats. Apart from these, histopathological changes also showed the protective nature of the MLT extract against APAP-induced necrotic damage of renal tissues. In conclusion, data suggest that the ethanol extract of MLT prevented renal damage from APAP-induced nephrotoxicity in rats and it is likely mediated through antioxidant activities. © 2010 Taylor & Francis.


Palani S.,Anna Bioresearch Foundation | Palani S.,PG Research | Raja S.,Bharat Institute of Technology | Naresh R.,Anna Bioresearch Foundation | Kumar B.S.,PG Research
Toxicology Mechanisms and Methods | Year: 2010

Plectranthus amboinicus (PA), commonly known as country borage, is a folkoric medicinal plant. Juice from its leaves is commonly used for illnesses including liver and renal conditions in the Asian sub-continent. Acetaminophen (APAP), used as an analgesic, produces liver and kidney necrosis in mammals at high doses. The aim of this study was to investigate the nephroprotective, diuretic, and antioxidant activities of the ethanol extract of PA at two doses of 250 and 500 mg/kg bw on APAP-induced toxicity in rats. This study shows that APAP significantly increases the levels of serum urea (UR), hemoglobin (Hb), total leukocyte count, creatinine, raised body weight, and reduced levels of neutrophils, granulocytes, uric acid, and platelet concentration. Ethanol extract of PA rescued these phenotypes by increasing anti-oxidative responses as assessed by biochemistry and histopathology. In addition, the ethanol extract of PA at two doses showed a significant diuretic activity by increased levels of total urine output and urinary elerolytes such as sodium and potassium. In conclusion, these data suggest that the ethanol extract of PA possess nephroprotective and antioxidant effects against APAP-induced nephrotoxicity and strong diuretics effect in rats. © 2010 Informa UK Ltd.


PubMed | Anna Bioresearch Foundation
Type: Journal Article | Journal: Natural product research | Year: 2011

Salacia oblonga, a woody climbing plant belonging to the family Celastaceae, is widely distributed in India and other southeast Asian countries. The genus Salacia have been used particularly for the treatment of diabetes, obesity, gonorrhoea, rheumatism, pruritus and asthma. Acetaminophen (APAP), used as an analgesic drug, produces liver and kidney necrosis in mammals at high doses. The aim of this study was to investigate the nephroprotective and antioxidant activities of the ethanol extract of Salacia oblonga (EESO) at the two dose levels of 250 and 500 mg/kgbw on APAP-induced toxicity in rats. The results showed that APAP significantly increases the levels of serum urea, creatinine, and reduces levels of uric acid concentration. The EESO reduces these by increasing anti-oxidative responses as assessed by biochemical and histopathological parameters. In conclusion, our results suggest that the EESO possesses nephroprotective and antioxidant effects against APAP-induced nephrotoxicity in rats.


PubMed | Anna Bioresearch Foundation
Type: Comparative Study | Journal: Waste management (New York, N.Y.) | Year: 2012

The petroleum fuel is nearing the line of extinction. Recent research and technology have provided promising outcomes to rely on biodiesel as the alternative and conventional source of fuel. The use of renewable source - vegetable oil constitutes the main stream of research. In this preliminary study, Waste Cooking Oil (WCO) was used as the substrate for biodiesel production. Lipase enzyme producing fungi Rhizopus oryzae 262 and commercially available pure lipase enzyme were used for comparative study in the production of Fatty Acid Alkyl Esters (FAAE). The whole cell (RO 262) and pure lipase enzyme (PE) were immobilized using calcium alginate beads. Calcium alginate was prepared by optimizing with different molar ratios of calcium chloride and different per cent sodium alginate. Entrapment immobilization was done for whole cell biocatalyst (WCB). PE was also immobilized by entrapment for the transesterification reaction. Seven different solvents - methanol, ethanol, n-propanol, n-butanol, iso-propanol, iso-butanol and iso-amyl alcohol were used as the acyl acceptors. The reaction parameters like temperature (30C), molar ratio (1:3 - oil:solvent), reaction time (24 h), and amount of enzyme (10% mass ratio to oil) were also optimized for methanol alone. The same parameters were adopted for the other acyl acceptors too. Among the different acyl acceptors - methanol, whose reaction parameters were optimized showed maximum conversion of triglycerides to FAAE-94% with PE and 84% with WCB. On the whole, PE showed better catalytic converting ability with all the acyl acceptor compared to WCB. Gas chromatography analysis (GC) was done to determine the fatty acid composition of WCO (sunflower oil) and FAAE production with different acyl acceptors.

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