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Okuyucu A.,Ondokuz Mayis University | Salis O.,Ondokuz Mayis University | Alici O.,Gaziosmanpasa University | Ilkaya F.,Ondokuz Mayis University | And 6 more authors.
Acta Medica Mediterranea | Year: 2015

Introduction: The endothelium plays an important role in maintaining vascular structure and tonus; and endothelial cells are protective against the thrombosis and atherosclerosis. Cardiovascular risk factors, such as metabolic diseases, systemic and local inflammations, result in endothelial dysfunction. Nitric oxide is one of the endothelium derived substances that has an important role in maintaining the endothelial homeostasis. The asymmetric dimethylarginine (ADMA) is the endogenous inhibitor of the nitric oxide synthase. ADMA has been shown to impair the endothelial function and to be associated with hypercholesterolemia, hyperhomocysteinemia, and atherosclerosis. Ascorbic acid, a vitamin with reductant and antioxidant effects, can improve endothelial function and decrease the reactive oxygen species level. Our objective was to determine pathologically exogenous ADMA effect on endothelium of abdominal aorta, and to observe protective effect of antioxidant ascorbic acid. Materials and methods: The rats were divided in three groups: control, ADMA and ADMA + ascorbic acid. After administration of ADMA and ascorbic acid for 10 days, the blood was collected and abdominal aorta removed from the animals. Homocysteine, triglyceride, and cholesterol levels were measured by autoanalyzer. Abdominal aorta was evaluated histopathologically and the intimal thickness was measured using an imaging system. Result: The ADMA increased the intimal thickness (p=0.003), and ascorbic acid inhibited this increase (p=0.001). ADMA also increased the levels of triglyceride and cholesterol (p=0.008 and p=0.001), and the increased triglyceride and cholesterol levels are inhibited by ascorbic acid (p=0.000 and p=0.001). Conclusions: Increased intimal thickness by delivering exogenous ADMA supports that exogenous ADMA might be one of the factors resulting in atherosclerosis. We consider that the increase in intima thickness may not be a direct effect of ADMA but may be secondary to the increased cholesterol levels and ascorbic acid can be protective for atherosclerosis.

Akyol S.,Ankara University | Akyol S.,Gaziosmanpasa University | Ugurcu V.,Private Bilecik Orhangazi Dialysis Center | Altuntas A.,Ankara Branch of Council of Forensic Medicine | And 3 more authors.
Scientific World Journal | Year: 2014

Caffeic acid phenethyl ester (CAPE), an active component of propolis, has been attracting the attention of different medical and pharmaceutical disciplines in recent years because of its antioxidant, anti-inflammatory, antiproliferative, cytotoxic, antiviral, antifungal, and antineoplastic properties. One of the most studied organs for the effects of CAPE is the kidney, particularly in the capacity of this ester to decrease the nephrotoxicity induced by several drugs and the oxidative injury after ischemia/reperfusion (I/R). In this review, we summarized and critically evaluated the current knowledge regarding the protective effect of CAPE in nephrotoxicity induced by several special medicines such as cisplatin, doxorubicin, cyclosporine, gentamycin, methotrexate, and other causes leading to oxidative renal injury, namely, I/R models and senility. © 2014 Sumeyya Akyol et al.

Karapirli M.,Ankara Branch of Council of Forensic Medicine | Kandemir E.,Council of Forensic Medicine | Akyol S.,Fatih University | Kantarci M.N.,Council of Forensic Medicine | And 3 more authors.
Journal of Forensic and Legal Medicine | Year: 2013

Carbon monoxide (CO) is produced by incomplete combustion of organic compounds. Its intoxication usually results from inhalation of fumes from improper heating stoves, motor vehicles, or smoke from fires. It can reversibly bind various heme-containing proteins such as hemoglobin, myoglobin, cytochrome p450 and cytochrome oxidases. Among them, it has a high affinity for hemoglobin (230-270 times more avidly than oxygen) with which it forms carboxy-hemoglobin (HbCO) leading to decrease in oxygen-carrying capacity followed by end-organ hypoxia. A tissue hypoxia may then result in transient or permanent damage of important organs like central nervous system and even death. CO poisonings from different reasons are an important cause of morbidity and mortality in Turkey. The purpose of this study on CO poisonings in Turkey is to sum up and analyze the best available researches in both forensic and clinical toxicology fields. This was achieved by synthesizing the results of Turkish and English papers on forensic and clinical CO poisoning cases conducted in universities, institutes, hospitals and other official or private organizations in Turkey. Total of 47,523 medico-legal autopsy data collected from 9 different forensic medicine branches and emergency rooms in different year intervals were reviewed and 980 CO poisoning cases were determined. To calculate the frequency of CO poisoning within all the autopsies carried out and fatal poisoning cases, the number of cases was divided by the total number of medico-legal autopsies and total fatal poisoning cases. The mean age of cases, the source of CO gas, the most common seasons and months, gender, and blood HbCO levels were also taken into consideration if the data is available. Under the light of the findings, we may suggest that determination of affecting factors in CO poisoning is going to be one of the key subjects for planning preventive interventions. © 2012 Elsevier Ltd and Faculty of Forensic and Legal Medicine. All rights reserved.

Celik S.,Council of Forensic Medicine | Karapirli M.,Ankara Branch of Council of Forensic Medicine | Kandemir E.,Council of Forensic Medicine | Ucar F.,Etlik Ihtisas Training and Research Hospital | And 4 more authors.
Journal of Forensic and Legal Medicine | Year: 2013

Methyl and ethyl alcohol poisoning are still responsible for high morbidity and mortality rates. The purpose of this retrospective study was to examine ethyl and methyl alcohol poisoning related deaths in Ankara and surrounding cities between 2001 and 2011 and compare them with previous studied conducted in Turkey and other countries. For this purpose, 10,720 medico-legal autopsy cases performed in Ankara Branch of the Council of Forensic Medicine were reviewed in terms of alcohol poisonings. The deaths due to methanol and ethanol poisoning were 74 (0.69% of all medico-legal autopsies performed) and the distribution among them was 35 (47.3%) for methanol poisoning and 39 (52.7%) for ethanol poisoning. Overwhelming majority of the cases were male (n = 67, 90.5%). The mean age of the victims was 44.9 ± 10.9 years and ranging from 21 to 92 years. The age group of 35-49 years was the mostly affected. Most of the cases were seen in 2004 (n = 12, 16.2%). The levels of postmortem blood alcohol levels were available for all cases and the mean alcohol levels were 322.8 ± 155.5 mg/dL ranging from 74 to 602 mg/dL for methanol and 396.8 ± 87.1 mg/dL and ranging from 136 to 608 mg/dL for ethanol. Early diagnosis is essential for successful treatment in methanol and ethanol poisoning. Besides increased awareness, more sensitive/specific diagnostic tools, and the prompt approach to the poisoned individual should be implemented in the hospitals.

Duzgun A.,Ankara Branch of Council of Forensic Medicine | Bedir A.,Ondokuz Mayis University | Ozdemir T.,Gazi State Hospital | Nar R.,Aksaray State Hospital | And 4 more authors.
Indian Journal of Biochemistry and Biophysics | Year: 2013

The endoplasmic reticulum (ER) is related to the various signal routes that are activated in unfolded protein response (UPR). The Grp78, Grp94, CHOP, MTJ1 and HMOX1 genes expressions demonstrate UPR activity. In this study, we investigated the UPR gene expressions in larynx epidermoid carcinoma (HEp2) to which dexamethasone (dex) was applied. HEp2 cells were administered for 48 h with different combinations using 0.1 μM and 1 μM dex, 1 mM phenyl butyric acid (PBA) and 100 ng/ml lipopolysaccharide (LPS). The Grp78, Grp94, CHOP, MTJ1 and HMOX1 genes expression was determined using quantitative RT-PCR. The Grp78, MTJ1 and HMOX1 gene expression increased with the administration of 1 μM dex. CHOP expression, on the other hand, decreased with 0.1 μM dex. When dex was combined with LPS, nearly all gene expressions decreased. The increase in Grp78, Grp94, HMOX1 and MTJ1 gene expression was greater in groups in which dex was administered in combination with PBA than in groups in which dex was administered alone. Dex in low dose (0.1 μM) caused a decrease in CHOP expression in HEp2 cells and an increase in Grp78 expression, in particular. The changes in UPR genes expressions may lead to the extended survival of the cells.

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