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We report on a new scheme for the determination of the activity of caspase-3 using a specific peptide labeled with N-(4-aminobutyl)-N-ethylisoluminol (ABEI) as a chemiluminescent (CL) probe and on the development of magnetic separation technology. Firstly, the ABEI-labeled and biotinylated peptide was prepared and conjugated to streptavidin-coated magnetic beads (MBs) to form f-MBs (functionalized magnetic beads). The f-MBs contain a site (DEVD, Asp-Glu-Val-Asp) that is cleaved by caspase-3. Upon cleavage, the terminal residue attached to ABEI can dissociate from the f-MBs and can be used for CL detection. CL intensity is linearly related to the concentration of caspase-3 in the range 1. 0 to 600 ng mL -1, with a detection limit of 0. 3 ng mL -1. The relative standard deviation of the assay is 3. 6 % at a level of 50 ng mL -1 of caspase-3 (for n = 11). The CL assay has been applied to the determination of caspase-3 in Jurkat cell extract with recoveries between 96. 6 % and 106. 1 % (n = 5). © 2012 Springer-Verlag.

Zhao Y.,Ankang University
Proceedings of the 2013 International Conference on Advanced Information Engineering and Education Science, ICAIEES 2013 | Year: 2013

The integration of modern information technology and class teaching is the aim of the teaching reform of modern education. In this paper, the writer discusses the basic strategies of this integration from the perspective of the application of educational technology and the education culture development. © 2013. The authors - Published by Atlantis Press..

Wen Y.-N.,Ankang University
Central European Journal of Physics | Year: 2014

The surface relaxation and the formation of a single vacancy in very thin Cu (001) film formed by 2 ~ 14 atomic layers have been studied by using MAEAM and MD simulation. For the surface relaxtion, the highest surface energy is in the l = 2 atomic layers. The multilayer relaxation mainly occurs between the first two atomic layers, and the maximum contractive displacement is obtained in the very thin Cu (001) film formed by l = 3 atomic layers. For the vacancy formed in l′ = 1 of the very thin Cu (001) film formed by l = 2 ~ 14 layers, the most difficult site in the film formed by l = 3 atomic layers. © 2014 Versita Warsaw and Springer-Verlag Wien.

Luo W.-Q.,Ankang University | Chen J.-C.,Ankang University
Construction and Building Materials | Year: 2011

This paper presents a laboratory study of modified bitumen containing ethylene vinyl acetate (EVA) and ethylene vinyl acetate grafted maleic anhydride (EVA-g-MAH) copolymers. When the different polymers were added, the physical properties of modified bitumens, such as softening point, ductility at low temperature and storage stability at high temperature are changed. The polymer modified bitumens (PMBs) were analyzed by thermogravimetry (TG), derivative thermogravimetry (DTG) and fourier transform infrared spectroscopy (FTIR). Thermal characterizations showed that the main decomposition stage refers to samples with modifiers exhibited a slight increase in thermal stability and the sample with the highest stability was EVA-g-MAH PMBs. FTIR analysis suggested that it is difficult to prove the EVA-g-MAH has reacted with the functional group in bitumen by FTIR spectrum. The dynamic shear rheometer (DSR) tests reported that added content of EVA-g-MAH has great effect on the rheological properties of the binder and its high temperature performance was improved while its temperature susceptibility was reduced in compare to EVA modified bitumens binder. © 2010 Elsevier Ltd. All rights reserved.

Zhu Y.,Ankang University
Advanced Materials Research | Year: 2013

This paper analyzed efficiency performance of two most popular charge pumps: Linear Dickson charge pump and the Exponential Dickson charge pump. The analysis result indicated that in a certain range, exponential charge pump had a much higher efficiency than the linear one. With this analysis result, a novel exponential charge pump was proposed. The simulation result shows the proposed charge pump has a 18.4% improvement in efficiency. © (2013) Trans Tech Publications, Switzerland.

Wang Y.-W.,Ankang University
Jiegou Huaxue | Year: 2011

A new one-dimensional double chain coordination polymer, [Na(cbsH)(H 2O)3]n (cbsH = 4-carboxy-benzenesulfonic anion), has been synthesized by aqueous reaction, structurally described, and thermally characterized. It crystallizes in the triclinic system, space group P1 with a = 5.645(4), b = 7.905(5), c = 13.043(8) Å, α = 89.391(7), β = 80.637(7), γ = 82.845(7)°, V = 569.8(6) Å3, Z = 2, C7H11NaO8S, Mr = 278.21, Dc = 1.622 Kg/m3, F(000) = 288, R = 0.0260 and wR = 0.0697. The title compound consists of one-dimensional double chains. 4-Carboxy-benzenesulfonic acid acts as a bridging bidentate ligand to link two sodium(I) ions to form a binuclear structure. Remarkably, the sodium(I) ion behaves as a metal bridge so that the binuclear structure units are connected to construct a one-dimensional double chain polymer. Furthermore, a 3-D framework is assembled through intermolecular hydrogen bonds. Thermogravimetric analysis shows that this compound begins losing the free solvent molecules at 106 °C and decompounding completely at 580 °C.

A novel sulfur/multiwalled carbon nanotube nanocomposite (S/MWCNT) was prepared by a facile quasi-emulsion template method in an O/W system. Transmission and scanning electronic microscopy show the formation of a highly developed core-shell tubular structure consisting of S/MWCNT composite with uniform sulfur coating on its surface. The homogenous dispersion and integration of MWCNT in the S/MWCNT composite create a highly conductive and mechanically flexible framework, enhancing the electronic conductivity and consequently the rate capability of the material. The S/MWCNT composite cathode could deliver a stable discharge (the fifth cycle) capacity of about 903 mAh g-1 at 0.1 C, 751 mAh g-1 at 0.5 C, and 631 mAh g-1 at 1 C. © 2013 Springer-Verlag Berlin Heidelberg.

Liang J.Y.,Ankang University
Applied Mechanics and Materials | Year: 2014

Edge detection is an old and hot topic in image processing, pattern recognition and computer vision. Numerous edge detection approaches have been proposed to gray images. It is difficult to extend these approaches to color image edge detection. A novel edge detection method based on mathematical morphology for color images is proposed in this paper. The proposed approach firstly compute vector gradient based on morphological gradient operators, and then compute the optimal gradient according to structure elements with different size. Finally, we use a threshold to binary the gradient images and then obtain the edge images. Experimental results show that the proposed approach has advantages of suppressing noise and preserving edge details and it is not sensitive to noise pixel. The finally edge images via the proposed method have high PSNR and NC compared with the traditional approaches. © (2014) Trans Tech Publications, Switzerland.

A novel mercury(II) ion (Hg2+) biosensor with electrogenerated chemiluminescence (ECL) detection using tris(2,2'-bipyridyl) ruthenium derivatives (ruthenium complex) as labeling was developed in the prescent work. One thymine (T)-rich single-strand DNA (ssDNA) labeled with a ruthenium complex was taken as an ECL probe. When the other T-rich capture ssDNA was self-assembled onto the surface of a gold electrode with a thiol group, and then hybridized with the ECL probe to form double-strand DNA (dsDNA) structures in the presence of Hg2+, a strong ECL response was electrochemically generated. The ECL intensity was linearly related to the concentration of Hg2+ in the range from 1.0 × 10-6 to 1× 10-9 M with a detection limit of 3.0 × 10-10 M. The relative standard deviation was 4.1% at 1.0 × 10-7 M Hg2+ (n = 5). This work demonstrates that the combination of the strongly binding T-rich DNA to Hg2+ with the highly sensitive ECL technique to design an ECL Hg2+ biosensor is a great promising approach for the determination of metal ions. © 2011 The Japan Society for Analytical Chemistry.

Numerous efforts have been made to improve the thermal resistance and chlorine resistance of nanofiltration (NF) membranes. In this work, interfacial polymerization (IP) is selected for preparing TFC membranes because TFC membranes prepared with the IP method can provide high fluxes. Poly(melamine/trimesoyl chloride (TMC)) with all aromatic rings and triazine rings is used to fabricate the functional layer of the composite NF membrane and a reinforced polyetherimde (PEI) ultrafiltration (UF) membrane is used as the support layer. The effects of the reaction time and the effects of the monomer concentration on the membrane performance are studied in detail in this work. When the melamine concentration is 2.0%, reaction time is 40s and the curing temperature is 70°C, the optimized preparation conditions of the composite membrane are reached. The flux of the composite NF membrane is about 34L/m2h and its Na2SO4 rejection is about 77.8% when it is tested at 1.0MPa and 20°C. The surface morphologies of the composite membrane and substrate membrane are observed by scanning electron microscopy (SEM). The prepared composite membrane shows excellent thermal resistance and chlorine resistance compared with the poly(piperazine amide/TMC) amide NF membrane prepared at the same condition. The flux of poly(melamine/TMC) composite membrane increases from 32 to 138L/m2h while the rejection of Na2SO4 does not decrease when the operation temperature is elevated from 18°C to 95°C. The performance of poly(melamine/TMC) amide does not decline during 96h immersion in chlorine solution (NaClO, 200ppm). © 2012 Elsevier B.V.

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