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Sheikhlou M.,University of Tabriz | Abbasi M.A.,Animal Science Research Institute of IRAN ASRI
Small Ruminant Research | Year: 2016

In this study a pedigree analysis was conducted to characterize the genetic structure and diversity of the closed nucleus of Iranian Lori-Bakhtiari sheep. Herdbook information collected between 1992 and 2014, was used to estimate generation interval, pedigree completeness level, inbreeding and its evolution during the studied years. Animals born between 2011 and 2014 were selected as a reference population for estimating parameters derived from probability of gene origins. The mean generation interval was 3.42 year in the studied period. Average inbreeding coefficient of the animals in the reference population was 1.02%. The rate of inbreeding was 0.17% per generation (P < 0.01) in the studied period. Realized effective population size estimated from individual increase in coancestry was 101. The effective number of founders, effective number of ancestors and founder genome equivalent of the reference population were 77, 43 and 21 respectively. The results indicated that population under study has fairly good genetic diversity. However, population has lost a part of its genetic diversity due to unbalanced contribution of founders, bottlenecks and genetic drift. For continued genetic gain, enlarging the selection basis and implementation of methods that maximize the selection response at an acceptable level of inbreeding are recommended. © 2016 Elsevier B.V. Source

Behdani M.,Pasteur Institute of Iran | Zeinali S.,Pasteur Institute of Iran | Khanahmad H.,Pasteur Institute of Iran | Karimipour M.,Pasteur Institute of Iran | And 7 more authors.
Molecular Immunology | Year: 2012

Vascular endothelial growth factor receptor-2 (VEGFR2) is an important tumor-associated receptor and blockade of the VEGF receptor signaling can lead to the inhibition of neovascularization and tumor metastasis. Nanobodies are the smallest intact antigen binding fragments derived from heavy chain-only antibodies occurring in camelids. Here, we describe the identification of a VEGFR2-specific Nanobody, named 3VGR19, from dromedaries immunized with a cell line expressing high levels of VEGFR2. We demonstrate by FACS, that 3VGR19 Nanobody specifically binds VEGFR2 on the surface of 293KDR and HUVECs cells. Furthermore, the 3VGR19 Nanobody potently inhibits formation of capillary-like structures. These data show the potential of Nanobodies for the blockade of VEGFR2 signaling and provide a basis for the development of novel cancer therapeutics. © 2011 Elsevier Ltd. Source

Tajabadi N.,University Putra Malaysia | Tajabadi N.,Animal Science Research Institute of IRAN ASRI | Ebrahimpour A.,University Putra Malaysia | Baradaran A.,University Putra Malaysia | And 5 more authors.
Molecules | Year: 2015

Dominant strains of lactic acid bacteria (LAB) isolated from honey bees were evaluated for their γ-aminobutyric acid (GABA)-producing ability. Out of 24 strains, strain Taj-Apis362 showed the highest GABA-producing ability (1.76 mM) in MRS broth containing 50 mM initial glutamic acid cultured for 60 h. Effects of fermentation parameters, including initial glutamic acid level, culture temperature, initial pH and incubation time on GABA production were investigated via a single parameter optimization strategy. The optimal fermentation condition for GABA production was modeled using response surface methodology (RSM). The results showed that the culture temperature was the most significant factor for GABA production. The optimum conditions for maximum GABA production by Lactobacillus plantarum Taj-Apis362 were an initial glutamic acid concentration of 497.97 mM, culture temperature of 36°C, initial pH of 5.31 and incubation time of 60 h, which produced 7.15 mM of GABA. The value is comparable with the predicted value of 7.21 mM. © 2015 by the authors; licensee MDPI. Source

Tajabadi N.,University Putra Malaysia | Tajabadi N.,Animal Science Research Institute of IRAN ASRI | Baradaran A.,University Putra Malaysia | Ebrahimpour A.,University Putra Malaysia | And 5 more authors.
Microbial Biotechnology | Year: 2015

Gamma-aminobutyric acid (GABA) is an important bioactive compound biosynthesized by microorganisms through decarboxylation of glutamate by glutamate decarboxylase (GAD). In this study, a full-length GAD gene was obtained by cloning the template deoxyribonucleic acid to pTZ57R/T vector. The open reading frame of the GAD gene showed the cloned gene was composed of 1410 nucleotides and encoded a 469 amino acids protein. To improve the GABA-production, the GAD gene was cloned into pMG36e-LbGAD, and then expressed in Lactobacillus plantarumTaj-Apis362 cells. The overexpression was confirmed by SDS-PAGE and GAD activity, showing a 53KDa protein with the enzyme activity increased by sevenfold compared with the original GAD activity. The optimal fermentation conditions for GABA production established using response surface methodology were at glutamic acid concentration of 497.973mM, temperature 36°C, pH 5.31 and time 60h. Under the conditions, maximum GABA concentration obtained (11.09mM) was comparable with the predicted value by the model at 11.23mM. To our knowledge, this is the first report of successful cloning (clone-back) and overexpression of the LbGAD gene from L.plantarum to L.plantarum cells. The recombinant Lactobacillus could be used as a starter culture for direct incorporation into a food system during fermentation for production of GABA-rich products. © 2015 The Authors. Microbial Biotechnology published by John Wiley & Sons Ltd and Society for Applied Microbiology. Source

Rafeie F.,Islamic Azad University at Tehran | Amirinia C.,Animal Science Research Institute of IRAN ASRI | Javaremi A.N.,University of Tehran | Mirhoseini S.Z.,Guilan University | Amirmozafari N.,Tehran University of Medical Sciences
African Journal of Biotechnology | Year: 2011

Autosomal markers and mtDNA have been used in horse phylogenetic studies. These studies display evolutionary events that happened in both sexes or only in females. It is necessary to investigate genetic diversity in Y-specific markers for clarifying contribution of males in horse domestication. The Y chromosome, excluding the pseudoautosomal region, is inherited as a single nonrecombinant unit and, therefore, it warrants that mutational events in patrilines are preserved as single haplotypes. Six Yspecific microsatellites were used to study patrilineal genetic variation in 405 male horses from 8 Iranian native horse breeds, one wild population and an exotic breed. These markers displayed no variation in all populations. The lack of polymorphisms could be as a result of lower contribution of stallions to the gene pools of the domestic horses compared to the mares because a sex bias is towards females due to a special breeding strategy (in which a few selected stallions mate with many mares each), a strong tendency to upgrade many breeds by crossing between champion stallions from particular breeds and mares from different breeds; also due to a bias in the early utilization of male horses as food source, a bias towards stallions in migrations and lack of detailed maps on horse Y chromosome. © 2011 Academic Journals. Source

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