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Salehi M.,Animal Science Research Institute of Iran ASRI | Mirhadi A.,Animal Science Research Institute of Iran ASRI | Ghafouri-Kesbi F.,Islamic Azad University at Karaj | Fozi M.A.,Shahid Bahonar University of Kerman | Babak A.,Islamic Azad University at Karaj
Journal of Agricultural Science and Technology | Year: 2013

The aim followed in the present paper was to evaluate the slaughter body and carcass weights as well as the characteristics of hide and leather in Dromedaries (one-hump) and in the crossbred (C. bactrianus×C. droedarius) camels. Fourteen camels from each sex (female and male) representing two genotypes at 21 months of age were utilized. Slaughter weight, hot and cold carcass weights, dressing-out percentage, wet and dry salting hide and leather properties were assessed. There were significant differences in slaughter weights between Dromedary and crossed types (339±10.7 vs. 372±11.1 kg) and as well between male vs. female camels (382±9.7 vs. 326±10.6 kg). Moreover, while the effect of sex was significant on wet hide weight (34±1.2 vs. 29.3±1.2 kg for males and females, respectively), the difference observed between males and females regarding the thickness of hide was non-significant (P> 0.05). Breaking force, tensile strength and elongation of the leather samples decreased with increase in their thicknesses. It was found out that the obtained leather from the camel benefited from such valuable mechanical characteristics as tensile strength and extension with no variations being observed as due to the animal's sex or genotype.


Rafeie F.,Islamic Azad University at Tehran | Amirinia C.,Animal Science Research Institute of Iran ASRI | Javaremi A.N.,University of Tehran | Mirhoseini S.Z.,Guilan University | Amirmozafari N.,Tehran University of Medical Sciences
African Journal of Biotechnology | Year: 2011

Autosomal markers and mtDNA have been used in horse phylogenetic studies. These studies display evolutionary events that happened in both sexes or only in females. It is necessary to investigate genetic diversity in Y-specific markers for clarifying contribution of males in horse domestication. The Y chromosome, excluding the pseudoautosomal region, is inherited as a single nonrecombinant unit and, therefore, it warrants that mutational events in patrilines are preserved as single haplotypes. Six Yspecific microsatellites were used to study patrilineal genetic variation in 405 male horses from 8 Iranian native horse breeds, one wild population and an exotic breed. These markers displayed no variation in all populations. The lack of polymorphisms could be as a result of lower contribution of stallions to the gene pools of the domestic horses compared to the mares because a sex bias is towards females due to a special breeding strategy (in which a few selected stallions mate with many mares each), a strong tendency to upgrade many breeds by crossing between champion stallions from particular breeds and mares from different breeds; also due to a bias in the early utilization of male horses as food source, a bias towards stallions in migrations and lack of detailed maps on horse Y chromosome. © 2011 Academic Journals.


Ghafouri-Kesbi F.,Animal Science Research Institute of Iran ASRI | Abbasi M.A.,Animal Science Research Institute of Iran ASRI | Afraz F.,Animal Science Research Institute of Iran ASRI | Babaei M.,Animal Science Research Institute of Iran ASRI | And 2 more authors.
Tropical Animal Health and Production | Year: 2011

Genetic parameters for average daily gain from birth to weaning (ADGa), birth to 6 months (ADGb), weaning to 6 months (ADGc), weaning to yearling age (ADGd), and corresponding Kleiber ratios (KRa, KRb, KRc, and KRd) were estimated by using records of 3,533 Zandi lambs, descendent of 163 sires and 1265 dams, born between 1991 and 2005 at the Zandi Sheep Breeding Station at Khojir National Park, Tehran, Iran. A derivative-free algorithm combined with a series of six single-trait linear animal models was used to estimate phenotypic variance and its direct, maternal, and residual components. In addition, bivariate analyses were done to estimate (co)variance components between traits. Estimates of direct heritability (h2) were 0.11, 0.15, 0.09, 0.10, 0.10, 0.10, 0.06, and 0.07 for ADGa, ADGb, ADGc, ADGd, KRa, KRb, KRc, and KRd, respectively, thereby indicating the presence of low additive genetic variation for growth rate and Kleiber ratio in this population of Zandi sheep. Maternal genetic component was found to be significant on ADGa and KRa and contributed 3% and 5%, respectively, in total phenotypic variance of ADGa and KRa. A widespread range of genetic correlations among traits studied was observed. Except for negative genetic correlations between ADGa and KRc, ADGa and KRd, and between KRa and KRc, in other cases, genetic correlations were positive and moderate to very high. Phenotypic correlations ranged from -0.49 (ADGa/KRd) to 0.94 (ADGc/KRc). These results indicate that selecting for improved growth rate or Kleiber ratio in Zandi sheep would generate a relatively slow genetic progress. © 2011 Springer Science+Business Media B.V.


Behdani M.,Pasteur Institute of Iran | Zeinali S.,Pasteur Institute of Iran | Khanahmad H.,Pasteur Institute of Iran | Karimipour M.,Pasteur Institute of Iran | And 7 more authors.
Molecular Immunology | Year: 2012

Vascular endothelial growth factor receptor-2 (VEGFR2) is an important tumor-associated receptor and blockade of the VEGF receptor signaling can lead to the inhibition of neovascularization and tumor metastasis. Nanobodies are the smallest intact antigen binding fragments derived from heavy chain-only antibodies occurring in camelids. Here, we describe the identification of a VEGFR2-specific Nanobody, named 3VGR19, from dromedaries immunized with a cell line expressing high levels of VEGFR2. We demonstrate by FACS, that 3VGR19 Nanobody specifically binds VEGFR2 on the surface of 293KDR and HUVECs cells. Furthermore, the 3VGR19 Nanobody potently inhibits formation of capillary-like structures. These data show the potential of Nanobodies for the blockade of VEGFR2 signaling and provide a basis for the development of novel cancer therapeutics. © 2011 Elsevier Ltd.


Sheikhlou M.,University of Tabriz | Abbasi M.A.,Animal Science Research Institute of Iran ASRI
Small Ruminant Research | Year: 2016

In this study a pedigree analysis was conducted to characterize the genetic structure and diversity of the closed nucleus of Iranian Lori-Bakhtiari sheep. Herdbook information collected between 1992 and 2014, was used to estimate generation interval, pedigree completeness level, inbreeding and its evolution during the studied years. Animals born between 2011 and 2014 were selected as a reference population for estimating parameters derived from probability of gene origins. The mean generation interval was 3.42 year in the studied period. Average inbreeding coefficient of the animals in the reference population was 1.02%. The rate of inbreeding was 0.17% per generation (P < 0.01) in the studied period. Realized effective population size estimated from individual increase in coancestry was 101. The effective number of founders, effective number of ancestors and founder genome equivalent of the reference population were 77, 43 and 21 respectively. The results indicated that population under study has fairly good genetic diversity. However, population has lost a part of its genetic diversity due to unbalanced contribution of founders, bottlenecks and genetic drift. For continued genetic gain, enlarging the selection basis and implementation of methods that maximize the selection response at an acceptable level of inbreeding are recommended. © 2016 Elsevier B.V.


Seyedabadi H.R.,Animal Science Research Institute of Iran ASRI | Banabazi M.H.,Animal Science Research Institute of Iran ASRI | Afraz F.,Animal Science Research Institute of Iran ASRI | Asadzadeh N.,Animal Science Research Institute of Iran ASRI | And 2 more authors.
Journal of Animal and Veterinary Advances | Year: 2011

Severe Combined Immunodeficiency Disease (SCID) is an autosomal recessive hereditary disease occurring among Arabian horses. The genetic defect responsible for this disease was recently identified as a 5-basepair deletion in the gene encoding DNA-Protein Kinase catalytic subunit (DNA-PKcs). Horses with one copy of the gene appear normal while horses with two copies of the gene manifest the disease. The only way to avoid the economic losses is early detection of SCID carriers. Because the real value of this test is that breeders can use it to ensure that they will never produce a SCID foal and that they can by breeding carriers to clears, preserve the unique and positive characteristics of exceptional carriers. And thereby breed out the undesirable gene over several generations. © Medwell Journals, 2011.


Ghorbani Sh.,Ferdowsi University of Mashhad | Kamali M.A.,Animal Science Research Institute of Iran ASRI | Abbasi M.A.,Animal Science Research Institute of Iran ASRI | Ghafouri-Kesbi F.,Animal Science Research Institute of Iran ASRI
Journal of Agricultural Science and Technology | Year: 2012

This study was conducted to investigate direct and maternal effects on some economic traits in north Iranian native fowls. Traits considered were body weight at 1 day of age (BW1), body weight at 8 weeks of age (BW8), body weight at 12 weeks of age (BW12), egg number (EN) during the first 12 weeks of laying period, egg weight at 1st day of laying (EW1), mean egg weight at 28th, 30th and 32nd weeks (MEW), age at sexual maturity (ASM) and weight at sexual maturity (WSM). The data were analyzed using Restricted Maximum Likelihood procedure fitting an animal model with DFREML software package. A series of six different animal models with and without maternal effects were fitted for all traits, and the best model for each trait was chosen based on log-likelihood ratio tests (LRT). The direct heritability (h2) estimates ranged from 0.03 (BW1) to 0.51 (MEW). Maternal effects were significant on all the traits studied. The estimates of maternal heritability (m2) ranged from 0.01 (BW8, WSM, ASM, EW1) to 0.15 (BW1), and the estimates of maternal environmental variance as a proportion of phenotypic variance (c2) varied from 0.02 (WSM, EW1, EN) to 0.23 (BW1). The correlations between direct and maternal additive genetic effects (ram) were negative for all traits. These results indicate that genetic selection schemes for body weight and egg traits should utilize models incorporating both direct and maternal effects.


Tajabadi N.,University Putra Malaysia | Tajabadi N.,Animal Science Research Institute of Iran ASRI | Baradaran A.,University Putra Malaysia | Ebrahimpour A.,University Putra Malaysia | And 5 more authors.
Microbial Biotechnology | Year: 2015

Gamma-aminobutyric acid (GABA) is an important bioactive compound biosynthesized by microorganisms through decarboxylation of glutamate by glutamate decarboxylase (GAD). In this study, a full-length GAD gene was obtained by cloning the template deoxyribonucleic acid to pTZ57R/T vector. The open reading frame of the GAD gene showed the cloned gene was composed of 1410 nucleotides and encoded a 469 amino acids protein. To improve the GABA-production, the GAD gene was cloned into pMG36e-LbGAD, and then expressed in Lactobacillus plantarumTaj-Apis362 cells. The overexpression was confirmed by SDS-PAGE and GAD activity, showing a 53KDa protein with the enzyme activity increased by sevenfold compared with the original GAD activity. The optimal fermentation conditions for GABA production established using response surface methodology were at glutamic acid concentration of 497.973mM, temperature 36°C, pH 5.31 and time 60h. Under the conditions, maximum GABA concentration obtained (11.09mM) was comparable with the predicted value by the model at 11.23mM. To our knowledge, this is the first report of successful cloning (clone-back) and overexpression of the LbGAD gene from L.plantarum to L.plantarum cells. The recombinant Lactobacillus could be used as a starter culture for direct incorporation into a food system during fermentation for production of GABA-rich products. © 2015 The Authors. Microbial Biotechnology published by John Wiley & Sons Ltd and Society for Applied Microbiology.


Abbasi M.-A.,Animal Science Research Institute of Iran ASRI | Ghafouri-Kesbi F.,Animal Science Research Institute of Iran ASRI
Asian-Australasian Journal of Animal Sciences | Year: 2011

The aim of this paper was to estimate genetic parameters for body weight and five body measurements for an experimental population of Iranian Makooei sheep maintained at the Makooei Sheep Breeding Station at Makoo, Iran. To do this, yearling live weight (YW), and five body measurements, i.e., body length (BL), heart girth (HG), height at withers (HW), height at back (HB) and scrotal circumference (SC), were analyzed in a multi-trait animal model using the DXMUX program of DFREML software package. Heritability estimates were 0.22±0.08, 0.11±0.06, 0.21±0.07, 0.17±0.06, 0.17±0.06 and 0.32±0.10 for YW, BL, HG, HW, HB and SC, respectively. These estimates indicate that selection in Makooei sheep would generate moderate genetic progress in body weight and body measurements. Scrotal circumference, as an indicator of reproductive potential, exhibited the highest heritability. This trait, therefore, could successfully be used to increase productivity of males and, indirectly, female fertility. Genetic correlations between traits studied were all positive and ranged from 0.15 (YW/HB) to 0.99 (HW/HB). Phenotypic correlations were also positive and ranged from moderate (0.32, HW/SC) to high (0.94, HW/HB). Positive genetic and phenotypic correlations indicate that improvement in body measurements both at the genetic and phenotypic levels is expected through selection on body weight and vice versa.


Tajabadi N.,University Putra Malaysia | Tajabadi N.,Animal Science Research Institute of Iran ASRI | Ebrahimpour A.,University Putra Malaysia | Baradaran A.,University Putra Malaysia | And 5 more authors.
Molecules | Year: 2015

Dominant strains of lactic acid bacteria (LAB) isolated from honey bees were evaluated for their γ-aminobutyric acid (GABA)-producing ability. Out of 24 strains, strain Taj-Apis362 showed the highest GABA-producing ability (1.76 mM) in MRS broth containing 50 mM initial glutamic acid cultured for 60 h. Effects of fermentation parameters, including initial glutamic acid level, culture temperature, initial pH and incubation time on GABA production were investigated via a single parameter optimization strategy. The optimal fermentation condition for GABA production was modeled using response surface methodology (RSM). The results showed that the culture temperature was the most significant factor for GABA production. The optimum conditions for maximum GABA production by Lactobacillus plantarum Taj-Apis362 were an initial glutamic acid concentration of 497.97 mM, culture temperature of 36°C, initial pH of 5.31 and incubation time of 60 h, which produced 7.15 mM of GABA. The value is comparable with the predicted value of 7.21 mM. © 2015 by the authors; licensee MDPI.

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