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Yang Y.,Guizhou University | Yang Y.,Key Laboratory of Animal Genetics | Yang Y.,Guizhou Key Laboratory of Animal Genetics | Liu R.,Guizhou University | And 13 more authors.
Journal of Nanoscience and Nanotechnology | Year: 2013

Much tremendous break through have been obtained in recent years for nanopore sequencing to achieve the goal of $1000 genome. As a method of single molecule sequencing, nanopore sequencing can discriminate the individual molecules of the target DNA strand rapidly due to the current blockages by translocating the nucleotides through a nano-scale pore. Both the protein-pores and solid-state nanopore channels which called single nanopore sequencing have been studied widely for the application of nanopore sequencing technology. This review will give a detail representation to protein nanopore and solid-state nanopore sequencing. For protein nanopore sequencing technology, we will introduce different nanopore types, device assembly and some challenges still exist at present. We will focus on more research fields for solid-state nanopore sequencing in terms of materials, device assembly, fabricated methods, translocation process and some specific challenges. The review also covers some of the technical advances in the union nanopore sequencing, which include nanopore sequencing combine with exonuclease, hybridization, synthesis and design polymer. Copyright © 2013 American Scientific Publishers All rights reserved.

Yang Y.-Q.,Guizhou University | Hui Y.-T.,Guizhou University | Liu R.-Y.,Guizhou University | Jiao R.-G.,Animal Science and Technology Station of Guizhou | And 3 more authors.
Genetics and Molecular Research | Year: 2013

The STAM protein plays an important role in the cytokine-related JAK/STAT pathway. We selected the STAM2 gene as a candidate gene that could be linked to growth performance in analysis of a Chinese cattle breed (Wuchuan Black cattle). We examined genetic variants in the promoter region of the STAM2 gene and their associations with eight growth traits in 159 individuals. Seven SNPs, which included six new SNPs for the SNP database, were found. The core promoter region was identified with a bioinformatic software. This analysis also showed that the SNPs have a significant influence on the function and structure of the STAM2 promoter in terms of RNA secondary structure, CpG island, and transcription factor binding sites. Association analysis demonstrated that G-102A is significantly associated with withers height, heart girth, cannon circumference, chest width, and hip height in this population, which leads us to suggest that G-102A is a useful SNP marker for cattle growth performance. Animals with the genotype AA had higher mean values for withers height, cannon circumference, chest width, and hip height than those with GG and AG genotypes. This SNP of the STAM2 gene could be applied in marker-assisted selection for improving growth performance in cattle. © FUNPEC-RP.

Xie H.Q.,Guizhou University | Sun Y.Y.,Guizhou University | Pan D.X.,Guizhou University | Yang Y.Q.,Guizhou University | And 5 more authors.
Pakistan Journal of Agricultural Sciences | Year: 2015

STAT5A gene plays a significant role in growth traits by affecting JAK/STAT pathway. We identified two SNPs which were related to growth traits from 206 healthy and adult purebred individuals of Guizhou Black goats using the methods of direct sequencing and PCR-SSCP. The association analysis between genotypes and growth traits showed that the individuals of genotype TC were superior than CC in chest girth significantly (P<0.05), and the individuals of GA were significant superior than GG and AA in chest girth and body weight (P<0.05). Besides that, the heterozygotes had higher growth traits than those of homozygotes in general. Therefore, we made a conclusion that the two SNPs of STAT5A gene will serve as marker-assisted selection for improving growth traits in goats, but we should still continue to prove the validity of the conclusion by enlarging the total samples and breeds. © 2015, University of Agriculture. All rights reserved.

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