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Sawiress F.A.R.,Cairo University | Ziada M.S.,Animal Reproduction Research Institute | Bebawy W.S.F.,General Organization of Veterinary Services | Amer H.A.,National Research Center of Egypt
Endocrine Regulations | Year: 2011

Objective. It was aimed to investigate the effect of standardized ginseng extract on fertility parameters in diabetic rats. Methods. Thirty male rats were randomly allocated into three groups of 10 rats each: 1. controls, 2. diabetes (D) and 3. diabetes + ginseng (DG). The latter two groups were rendered diabetic by i.p. injection of streptozotocin (STZ; 50 mg/kg). Standardized ginseng extract (Dansk Droge A/S, Copenhagen, Denmark) was administered per os (100 mg/kg BW) by stomach tube daily for 90 days starting one week after STZ. Ninety days post STZ the rats were sacrificed, and testis, epididymis, prostate, and seminal vesicles were weighed and subjected to histological examination. In addition, spermiogram, testicular enzyme markers, intratesticular steroid hormonal profile and testicular antioxidant status were estimated. Results. The administration of ginseng extract resulted in a significant improvement of fertility parameters and testicular antioxidants together with a decrease in malondialdehyde and testicular pathological signs including degenerative changes of the seminiferous tubules. Conclusion. Ginseng extract may be a beneficial adjuvant therapy for diabetics suffering from infertility as a complication. Source

El-Maaty A.M.A.,National Research Center of Egypt | Sisy G.A.E.,National Research Center of Egypt | Shaker M.H.,Animal Reproduction Research Institute | Ezzo O.H.,National Research Center of Egypt
Asian Pacific Journal of Reproduction | Year: 2014

Objective: To study the effect of age and body fat on leptin levels and semen parameters of Arab horse. Methods: Fifteen fertile Arab stallions of different ages belonging to Police Academy were divided into three equal groups according to their age. Old horses are those of >18 yeas (18-27), Mid-age horses ≥13 to 18 years (13-18), Young horses are those of <12 years (7-11). Semen was evaluated three times for each stallion. Blood and seminal plasma were assayed for measuring leptin, testosterone and estradiol. Subcutaneous rump fat thickness was measured using ultrasound for estimating body fat percent and fat mass percent. Results: All body fat parameters were significantly high in Young stallions and decreased with increasing age. As age increased, testosterone levels increases but leptin levels decreased. Age was inversely correlated with fat %, fat mass and leptin. All fat parameters had direct correlation with leptin in semen and serum but an inverse one with serum testosterone. Serum leptin directly correlated with sperm cell concentration in Mid-age stallions and inversely correlated with percent of live sperm in Old stallions. Semen leptin correlated directly with both percent of live sperm and percent of abnormal sperm in Old stallions. Conclusion: This study proved that aging in stallions is related to a drop in fertility, a decrease in body fat and in turn leptin. Arab stallions of age 7 to 18 years could be used in the breeding efficiently. © 2014 Hainan Medical College. Source

Osman K.M.,Cairo University | Hassan H.M.,Animal Reproduction Research Institute | Ibrahim I.M.,Cairo University | Mikhail M.M.S.,Ministry of Agriculture
Comparative Immunology, Microbiology and Infectious Diseases | Year: 2010

Mammary gland secretions derived from secretory cows infected with coagulase +ve Staphylococcus spp. was examined for the expression of IL-6, production of lysozyme and NOx. The examined cows reflected 25 cases of subclinical mastitis and 15 cases of clinically mastitic animals. The IL-6 concentration in the subclinical animals was significantly higher (30.8 ng/ml) than the clinically manifested animals (18.0 ng/ml) and the normal cows (5.2 ng/ml). On the other hand the level of lysozyme although significantly higher than the normal cows (6.9 μg/ml) yet its level in the subclinical animals (11.2 μg/ml) was lower than that estimated in the clinical animals (15.6 μg/ml). Similarly, the level of NOx in the normal animals was found to be 5.6 μM/ml to increase to 6.2 μM/ml in the subclinical mastitic animals and to significantly increase further to 11.5 μM/ml in the clinically affected cows. These results suggest the promising use of whey IL-6, lysozyme or/and NO concentration variabilities as prognostic parameters on the degree of the commencement of mastitis in cows. © 2008 Elsevier Ltd. All rights reserved. Source

Shafik A.N.,Cairo University | Khodeir M.M.,Cairo University | Fadel M.S.,Animal Reproduction Research Institute
Journal of Cancer Science and Therapy | Year: 2011

Study background: Anticancer chemotherapy Anthracycline (ANT) antibiotics associated with cardiac and renal toxicity which represents serious complication. In this study, the protective effect of the 2 beta-blockers carvedilol and nebivolol were tested in a rat model of ANT induced cardio and renal-toxicity by repeated intraperitoneal doxorubicin (Dox) administration. Methods: Six groups of animals were used, each 8 rats divided as control group-received intraperitoneal saline every other day; control carvedilol: rats received carvedilol dose 30mg/kg/day orally; control nebivolol: rats received nebivolol 1mg/kg/day orally; doxorubicin alone: a dose of 3 mg/kg/day was administered intraperitoneal every other day; doxorubicin + carvedilol: carvedilol started at the same day with Dox; doxorubicin + nebivolol: nebivolol started at the same day with Dox. All substances were administered for 12 days. Detection & quantification of doxorubicin-induced heart and renal damage and therapeutic action of beta-blockers was done using Langendorff's technique, echo-cardiographic function examinations, serum creatinine, total proteins and histopathology. Results: The administration of doxorubicin in the dose of 3 mg/kg/day for 12 days produced pronounced heart impairment, as well as renal nephrotoxic changes. Significant reduction of Doxo-cardiotoxicity and nephrotoxicity in Nebivolol-treated animals more than Carvedilol treated animals. Conclusions: Coadministration of either carvedilol or nebivolol with doxorubicin was able to ameliorate up to almost contradict doxorubicin-induced myocardial injury, glomerular filtration disturbance and renal tubular damage with upper hand for nebivolol. So, they can be considered a feasible candidate to protect against nephrotoxicity & cardiotoxicity commonly encountered with doxorubicin treatment. © 2011 Shafik AN, et al. Source

Shehab-El-Deen M.A.M.M.,Ghent University | Shehab-El-Deen M.A.M.M.,Suez Canal University | Leroy J.L.M.R.,University of Antwerp | Fadel M.S.,Animal Reproduction Research Institute | And 3 more authors.
Animal Reproduction Science | Year: 2010

High yielding dairy cows experience a negative energy balance (NEB) early post-partum and it was hypothesized that this may be aggravated under summer heat stress (HS) conditions. In this study, which was performed in Egypt, 20 Holstein cows were followed during summer (n = 10) and winter (n = 10) seasons. All cows were multiparous and kept at the same herd. Blood was sampled from each cow starting 1 week before the expected calving date and then at 1-week intervals until week 6 post-partum. From week 2 to 6 post-partum follicular fluid was collected through transvaginal follicular fluid aspiration at 6 days intervals. Ambient air temperature (AT) and relative humidity (RH) were recorded and temperature-humidity index (THI) was calculated as well. Respiration rate (RR), rectal temperature (RT), and body condition score (BCS) were recorded for each cow at the time of blood sampling. Concentrations of glucose, insulin like growth factor-1 (IGF-1), non-esterified fatty acids (NEFA), urea and total cholesterol (TC) were measured in each blood and follicular fluid sample. All the cows showed a significantly higher RR and RT in summer (95.5 ± 1.1 and 39.88 ± 0.06, respectively) than in winter (43.89 ± 0.61 and 38.94 ± 0.07, respectively) (P < 0.001). Body condition score loss during the early post-partum period was higher in summer than in winter (1.1 ± 0.07 vs. 0.85 ± 0.06 point, respectively) (P < 0.001). The average dominant follicle diameter was significantly lower in summer than in winter during the period of negative energy balance (11.6 ± 0.7 mm vs. 15.3 ± 1.2 mm, respectively) (P < 0.01). Under summer heat stress, the concentrations of glucose (2.98 ± 0.07 and 2.19 ± 0.04 mmol/L), IGF-1 (106.7 ± 2.9 and 99.0 ± 3.4 ng/ml) and TC (137.3 ± 5.3 and 62.2 ± 5.1 mg/dl) in blood and FF, respectively, were significantly lower than winter concentrations by (0.17 ± 0.03 mmol/L, P < 0.001 and 0.26 ± 0.06 mmol/L, P < 0.001), (12.3 ± 3.6 ng/ml, P < 0.001 and 9.0 ± 2.7 ng/ml, P < 0.001) and (20.7 ± 1.8 mg/dl, P < 0.001 and 7.3 ± 1.1 mg/dl, P < 0.01), respectively. However, the concentrations of NEFA (0.68 ± 0.14 and 0.22 ± 0.02 mmol/L) and urea (9.27 ± 0.34 and 9.96 ± 0.25 mmol/L) in blood and FF, respectively, were significantly higher in summer compared to winter (0.50 ± 0.08 mmol/L, P < 0.001 and 0.20 ± 0.02 mmol/L, P < 0.001) and (8.77 ± 0.23 mmol/L, P < 0.05 and 8.96 ± 0.29 mmol/L, P < 0.001), respectively, throughout the experimental period. The results of the present study indicate that heat stress early post-partum aggravates NEB in high yielding dairy cows, reduces BCS, dominant follicle diameter and alters the biochemical concentrations in the follicular fluid of the dominant follicle which may result in inferior oocyte and granulosa cell quality and hence poorer fertility. © 2009 Elsevier B.V. All rights reserved. Source

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