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Sirotkin A.V.,Animal Production Research Center Nitra | Sirotkin A.V.,Constantine the Philosopher University | Sirotkin A.V.,Institute of Animal Genetics and Reproduction
International Journal of Biochemistry and Cell Biology | Year: 2011

The present short review demonstrates an important role of different cytokines (colony stimulating factors, tumor necrosis factors, interleukins, anti-Mullerian hormone, inhibin, activin, follistatin, bone morphogenetic proteins, growth and differentiation factors) in the control of different ovarian functions - ovarian cell proliferation, apoptosis, folliculogenesis, luteogenesis, oogenesis, release of hormones, response to upstream hormonal regulators, fertility and, in some cases, in development of ovarian disorders. The possibility of practical application of these molecules for characterization, prediction and regulation of the ovarian state including treatment of ovarian disorders is demonstrated. © 2011 Elsevier Ltd. Source

Roychoudhury S.,Assam University | Bulla J.,Slovak University of Agriculture | Sirotkin A.V.,Animal Production Research Center Nitra | Kolesarova A.,Slovak University of Agriculture
Journal of Environmental Science and Health - Part A Toxic/Hazardous Substances and Environmental Engineering | Year: 2014

Objective of this in vitro study was to examine the secretion activity (progesterone and insulin-like growth factor I) of porcine ovarian granulosa cells after copper (Cu) addition and to outline a potential intracellular mediator (cyclin B1) of its effects. It also aimed at investigating the apoptotic potential of Cu on porcine ovarian granulosa cells after addition in vitro. Ovarian granulosa cells were incubated with copper sulphate (CuSO 4·5H2O) at the doses 0.33, 0.40, 0.50, 1.0 and 2.0 μL mL-1 for 18 h and compared with control group without Cu addition. Release of progesterone (P4) and insulin-like growth factor I (IGF-I) by granulosa cells was assessed by RIA, expression of cyclin B1 by immunocytochemistry and apoptosis by TUNEL assay. Observations show that P 4 release by granulosa cells was inhibited while the release of IGF-I and cyclin B1 was stimulated significantly (P < 0.05) by CuSO 4·5H2O addition at the dose 2.0 μL mL -1. Also, addition of CuSO4.5H2O at the lowest dose used in the study (0.33 μL mL-1) significantly (P < 0.05) decreased apoptosis in granulosa cells. In conclusion, results indicate dose dependent effect of Cu on (1) secretion of steroid hormone progesterone and growth factor IGF-I, (2) expression of cyclin B1 as marker of proliferation of porcine ovarian granulosa cells, (3) apoptosis of porcine ovarian granulosa cells and, (4) that the effect of Cu on ovarian cell proliferation could be mediated by IGF-I and cyclin B1. Obtained data suggest interference of Cu in the pathways of proliferation of porcine ovarian granulosa cells through hormonal and intracellular peptide cyclin B1. © 2014 Copyright Taylor and Francis Group, LLC. Source

Soch M.,University of South Bohemia | Broucek J.,Animal Production Research Center Nitra | Srejberova P.,University of South Bohemia
Biologia | Year: 2011

The efficiency of sheep is dependent on their health and well-being. The blood markers can be critical for improving of the physiological, nutritional and pathological status of sheep organism. The aim of this study was to test the hypotheses that the red and white blood cells and copper (Cu) and zinc (Zn) plasma contents are impacted by altitude and season. The ewes were kept at three farms. Blood samples were divided according to factors of altitude (550 m, 800 m, 950 m above sea level), season (spring, fall) and year. The lowest haemoglobin concentration and value of haematocrit were detected at the altitude of 550 m (66.95 g L-1, 0.36 L L-1) and the highest at the altitude of 950 m (117.96 g L-1, 0.39 L L-1) (P <0.001). Spring values of haemoglobin and haematocrit were lower than fall values. The highest count of leucocytes was recorded at the altitude 950 m (9.57 G L-1), higher counts were contained in spring (P <0.001). The lowest percentage of eosinophiles was found at the altitude of 800 m (5.81%) and the highest at the altitude of 550 m (9.26%) (P <0.01). Phagocytose activities were the highest at the altitude of 950 m (95.07%) and the lowest at the altitude of 550 m (85.04%) (P <0.001). Phagocytose activities were higher in fall than in spring. The highest Cu concentration was found at the altitude of 550 m and the lowest at the altitude of 800 m (17.04 μmol L-1 vs. 14.37 μmol L-1). Zn levels were higher at altitudes of 950 m and 800 m than at the altitude of 550 m (17.81 μmol L-1, 17.00 μmol L-1 vs. 14.77 μmol L-1). We concluded that hematological markers and trace mineral content in grazed sheep may be impacted by altitude and season. © 2011 Versita Warsaw and Springer-Verlag Wien. Source

Krupova Z.,Animal Production Research Center Nitra | Michalickova M.,Animal Production Research Center Nitra | Krupa E.,Institute of Animal Science
Journal of Central European Agriculture | Year: 2012

The objective of this work was to synthesise and analyse the methodologies and the biological aspects of the costs calculation in ruminants in Slovakia. According to literature, the account classification of cost items is most often considered for construction of costing formula. The costs are mostly divided into fixed (costs independent from volume of herd's production) and variable ones (costs connected with improvement of breeding conditions). Cost for feeds and beddings, labour costs, other direct costs and depreciations were found as the most important cost items in ruminants. It can be assumed that including the depreciations into costs of the basic herd takes into consideration the real costs simultaneously invested into raising of young animals in the given period. Costs are calculated for the unit of the main and by-products and their classification is influenced mainly by the type of livestock and production system. In dairy cows is usually milk defined as the main product, and byproducts are live born calf and manure. The base calculation unit is kilogram of milk (basic herd of cows) and kilogram of gain and kilogram of live weight (young breeding cattle). In suckler cows is a live-born calf the main product and manure is the by-product. The costs are mostly calculated per suckler cow, live-born calf and per kilogram of live weight of weaned calf. Similar division of products into main and by-products is also in cost calculation for sheep categories. The difference is that clotted cheese is also considered as the main product of basic herd in dairy sheep and greasy wool as the by-products in all categories. Definition of the base calculation units in sheep categories followed the mentioned classification. The value of a by-product in cattle and sheep is usually set according to its quantity and intraplant price of the by-product. In the calculation of the costs for sheep and cattle the "structural ewe" and "structural cow" proportionally integrate the costs of other categories of the herd into the costs of the basic herd. Subtraction, allocation and combined calculation methods of costs are the most used in breeding of ruminants. It is recommended to consider specific breeding conditions and objective calculation of costs for the relevant category, when picking out the method of calculation. Source

Cikos S.,Slovak Academy of Sciences | Fabian D.,Slovak Academy of Sciences | Makarevich A.V.,Animal Production Research Center Nitra | Chrenek P.,Animal Production Research Center Nitra | And 2 more authors.
Human Reproduction | Year: 2011

Background The involvement of biogenic monoamines in early ('preneural') embryogenesis has been well documented in lower vertebrates, but much less information is available about the role of these molecules in the earliest stages of development in mammals, including humans. Methods Databases (PubMed, ISI Web of Knowledge and Scopus) were searched for studies relating to biogenic monoamines functioning in early embryos. The available data on the expression of histamine, serotonin and adrenergic receptors during mammalian preimplantation development were summarized, and the potential roles of biogenic monoamines in very early pregnancy were discussed. Results The roles of biogenic monoamines in mammalian preimplantation embryo development can be diverse, depending on the embryo developmental stage, and the physiological status of the maternal organism. Several receptors for biogenic monoamines are expressed and biologically functional in cells of preimplantation embryos. Activation of histamine receptors can play a role in embryo implantation and trophoblast invasion. Activation of adrenergic and serotonin receptors can influence proliferation and survival of early embryonic cells. Conclusions Biogenic monoamines can play an important role in physiological conditions, contributing to embryo-maternal interactions, or can influence the early embryo under unfavorable or pathological conditions (e.g. in maternal stress, or in women taking certain antidepressants, anti-migraine or anti-ulcer drugs). © 2011 The Author. Source

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