Animal Physiology and Physiological Chemistry

Stara Zagora, Bulgaria

Animal Physiology and Physiological Chemistry

Stara Zagora, Bulgaria

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Slavov E.,Trakia University | Georgiev I.P.,Animal Physiology and Physiological Chemistry | Dzhelebov P.,Trakia University | Kanelov I.,Animal Physiology and Physiological Chemistry | And 3 more authors.
Veterinary Research Communications | Year: 2010

As obesity is a state of low-grade inflammation, we aimed to investigate the combined effect of high-fat diet and bacterial infection on β-cell function and insulin sensitivity in dogs. We used 20 healthy, male, mongrel dogs randomly divided into four groups: control group-healthy, non-obese dogs; infected group-non-obese dogs with experimentally induced infection (Staphylococcus intermedius); obese group-obese dogs (after 90 day high-fat diet) and obese-infected group-obese dogs with experimentally induced infection (Staphylococcus intermedius). To evaluate insulin sensitivity and β-cell function an intravenous glucose tolerance test (IVGTT) was performed. Plasma insulin increased in all group after glucose infusion. The lowest values were found in obeseinfected group. Blood glucose also increased on 3 min after glucose infusion and then gradually decreased. In obese-infected group glucose concentration on 30 min was still significantly higher than initial levels, while in other groups glucose concentration returned to the initial values. The lowest rate of glucose elimination was found in infected group. In dogs of obese group and obese-infected group AUCins 0-60 min was lower compared to controls. AUCglucose 0-60 min values were lowest in infected group, while in obese-infectd group values were the highest. Levels of ΔI/ΔG in dogs of obese-infected group were significantly lower compared to controls and infected group. In conclusion, these results reveal that infection in obese dogs leads to impaired glucose tolerance, which is result of impairment in both insulin secretion and insulin sensitivity. © Springer Science+Business Media B.V. 2010.


Yaneva Z.,Animal Physiology and Physiological Chemistry | Georgieva N.,Animal Physiology and Physiological Chemistry
Macedonian Journal of Chemistry and Chemical Engineering | Year: 2013

The mechanism of Congo red (CR) biosorption by the agricultural waste material ball-milled maize cob (BMMC) biomass of Zea mays was studied by analyzing the effect of pH and biosorbent surface chemistry; the equilibrium and kinnetc behavior of the sorbate/sorbent system were also investigated. Surface chemistry and morphology were characterized by potentiometric titration, pH of zero charge, FTIR analyses and digital microscopy (DM). The acidic and basic sites for the biomass were quantified as 3.68 and 5.25 mmol g-1, respectively; therefore, the surface of the biomass was basic. The analysis of dye equilibrium isotherm data was done using the Langmuir, Freundlich and Redlich-Peterson models. CR biosorption on the agricultural waste biomaterial was mainly limited by chemisorption and/or intraparticle diffusion. The studies revealed that CR removal involved electrostatic interactions between negatively charged dye SO3 - groups and positively charged adsorbent surfaces, H-bonding between the oxygenand nitrogen-containing functional groups of CR and the BMMC surface and hydrophobic-hydrophobic interactions between the dye and sorbent hydrophobic parts. The maximum biosorption capacity of Zea mays biomass (q 4.83 mg g-1) occurred at pH 7.

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