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Karnāl, India

Pathan M.M.,Animal physiology
Revista Veterinaria | Year: 2010

Periparturient period is especially critical for health and subsequent productivity of buffaloes. The present study was undertaken to assess the degree of oxidative stress in Mehsana buffaloes during periparturient period. The results of the present study revealed that the antioxidant markers i.e. Melendealdehide (MDA), Super oxide dismutase (SOD) and Glutathione peroxidase (GSH-Px) were significantly (p<0.01) increased from 30 days before parturition attaining highest levels on the day of parturition. Significantly (p<0.01) decreased levels of these marker/enzymes were observed from 15 days after parturition to 30 days after parturition. This indicates higher oxidative stress and lower anti-oxidant status during pregnancy and parturition as significantly decreased level of MDA, SOD and GSH-Px were observed in non-pregnant buffaloes compared to pregnant buffaloes on the day of parturition. The study indicated higher level of oxidative stress in buffaloes during parturition than non-pregnant buffaloes. The levels of these markers observed at 30 days after parturition was higher than non-pregnant buffaloes. This might be due to stress imposed by heavy lactation and incomplete involution of uterus in pregnant buffaloes. Source


Bernaerts F.,University of Liege | Talavera J.,University of Murcia | Leemans J.,University of Liege | Hamaide A.,University of Liege | And 3 more authors.
Veterinary Journal | Year: 2010

The clinical features of brachycephalic airway obstructive disease in 11 brachycephalic dogs are described in this study. The respiratory strategy was assessed before (n = 11) and after (n = 6) surgery using barometric whole-body plethysmography (BWBP), with the relationship between BWBP variables and the severity of the clinical signs assessed by the use of a respiratory score based on clinical, radiographic and endoscopic findings. Partial collapse of the left main bronchus was a common finding not previously described as part of the brachycephalic airway obstructive disease syndrome. Epiglottic cysts, laryngeal granulomas and nasopharyngeal turbinates in English Bulldogs were other previously unreported findings. No significant correlation between the respiratory score and any of the BWBP variables was detected. Compared to healthy dogs, brachycephalic dogs had a significantly lower Te/Ti ratio (expiratory time over inspiratory time), peak inspiratory flow (PIF) per kg bodyweight (BW), significantly higher peak expiratory flow (PEF) per kg BW, PEF/PIF, and enhanced pause. These variations are compatible with upper airway obstructions primarily in the extrathoracic airways. Following surgery, a significant decrease in PEF/PIF was detected. The study showed that BWBP could be used to characterise the respiratory strategy in brachycephalic dogs before and after surgery. © 2008 Elsevier Ltd. All rights reserved. Source


Paulo N.M.,Federal University of Goais | Malmonge S.M.,Federal University of ABC | De Menezes L.B.,Federal University of Goais | De Lima F.G.,Federal University of Goais | And 5 more authors.
Acta Cirurgica Brasileira | Year: 2010

Purpose: To verify if the composit poli (2-hydroxyethyl methacrylate)-PolyHEMA/polypropylene mesh implanted in the female rat's abdominal wall could be suitable for the prevention of peritoneal adhesions, and for the evaluation of the tecidual response produced by this biomaterial. Methods: Polypropylene meshes (Group PP, n=20) and polypropylene meshes coated with a layer of poli (2-hydroxyethyl methacrylate)-PolyHEMA (Group PH, n=20) were implanted on the abdominal wall of Wistar female rats. Ten animals from each group were submitted to euthanasia at 15 and 30 days of the postoperative period. Results: The animals from the group PP presented visceral adhesions on the mesh surface, which was not observed in the ones from group PH. At the histopathological examination foreign body response was observed in both groups, whilst there was a greater intensity of inflammatory response in group PH on both moments. Conclusion: The poli (2-hydroxyethyl methacrylate) polyHEMA hydrogel associated to polypropylene mesh reduces visceral adhesion formation in rats, although it may be associated to greater inflammatory reaction. Source


Derlig K.,Institute for Biochemistry | Giessl A.,Animal physiology | Brandstatter J.H.,Animal physiology | Enz R.,Institute for Biochemistry | Dahlhaus R.,Institute for Biochemistry
Cell and Tissue Research | Year: 2015

Chromatin organization and the management of transcription and splicing are fundamental to the correct functioning of every cell but, in particular, for highly active cells such as photoreceptors, the sensory neurons of the retina. Rod photoreceptor cells of nocturnal animals have recently been shown to have an inverted chromatin architecture compared with rod photoreceptor cells of diurnal animals. The heterochromatin is concentrated in the center of the nucleus, whereas the genetically active euchromatin is positioned close to the nuclear membrane. This unique chromatin architecture suggests that the transcription and splicing machinery is also subject to specific adaptations in these cells. Recently, we described the protein Simiate, which is enriched in nuclear speckles and seems to be involved in transcription and splicing processes. Here, we examine the distribution of Simiate and nuclear speckles in neurons of mouse retinae. In retinal neurons of the inner nuclear and ganglion cell layer, Simiate is concentrated in a clustered pattern in the nuclear interior, whereas in rod and cone photoreceptor cells, Simiate is present at the nuclear periphery. Further staining with markers for the transcription and splicing machinery has confirmed the localization of nuclear speckle components at the periphery. Comparing the distribution of nuclear speckles in retinae of the nocturnal mouse with the diurnal degu, we found no differences in the arrangement of the transcription and splicing machinery in their photoreceptor cells, thus suggesting that the organization of these machineries is not related to the animal’s lifestyle but rather represents a general characteristic of photoreceptor organization and function. © 2015, Springer-Verlag Berlin Heidelberg. Source

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