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Nanaimo, Canada

Lovy J.,Aquatic Animal Health Unit | Savidant G.P.,University of Prince Edward Island | Wright G.M.,University of Prince Edward Island
Cell and Tissue Research

The ontogeny and disease responses of Langerhans-like cells within lymphoid tissues of Atlantic salmon, Salmo salar, and rainbow trout, Oncorhynchus mykiss, were investigated. These cells were studied in situ with the use of two markers: the ultrastructural presence of Birbeck-like granules and immunohistochemistry with an antibody against human langerin/CD207 that cross-reacts with salmonid tissues. The appearance of Birbeck-like granules was observed in rainbow trout at 2 weeks post-hatch (PH) in the thymus and anterior kidney prior to the development of the spleen. Spleen first appeared at 3 weeks PH in both Atlantic salmon and rainbow trout, and Birbeck-like granules were observed within cells of the newly developed spleens. The cross-reactivity of langerin as seen by immunohistochemistry was not clearly observed in kidney and spleen until 9 weeks PH, when a strong cytoplasmic reaction was observed. To study langerin-positive cells in spleen and kidney during disease, microsporidial gill disease (MGD) in rainbow trout was used as a known disease model inducing a strong cell-mediated adaptive immune response. Langerin-positive cells in healthy fish were seen predominantly in the spleen, and only low numbers were present in the anterior kidney. During MGD, langerin-positive cell numbers were elevated in the anterior kidney and were significantly higher during 5, 6, and 10 weeks post-exposure (PE) compared with healthy control tissue. During MGD, the distribution of langerin-positive cells in the spleen and anterior kidney shifted from having significantly higher numbers of cells in the spleen than in the kidney in controls and at 1 and 4 weeks PE to having a similar distribution of the cells in the two organs at 2, 3, 5, and 6 weeks PE. By 10 weeks PE, significantly higher numbers of langerin-positive cells occurred in the anterior kidney compared with the spleen. © 2011 Springer-Verlag. Source

Mahjoor A.A.,Islamic Azad University at Kazeroon | Akhlaghi M.,Aquatic Animal Health Unit
Asian Journal of Animal Sciences

Enteric redmouth disease is a serious infectious disease in salmonids that causes important economic losses in many countries. Since last few years there were reports on the occurrence of the disease in Iran based on the clinical signs. The disease caused great losses in rainbow trout farms in Fars Province, Iran. Biochemical test of the isolated Yersinia ruckeri resembled high degree of similarity between the isolate and the Yersinia ruckeri cited in the literature as serotype O1. Diseased fish showed typical hemorrhages, blackening of skin, exophthalmia and wide hemorrhages on internal organs. By histopathological examination, diseased fish showed generalized bacterimia with inflammation, glomerulonephritis and necrotic foci in kidney, necrosis and mild telangiectasis in liver, telangiectasis in spleen, hemorrhages and hyperemia in the intestinal mucosa, myocardial degeneration, atrophy and edema in heart, atrophy of pancrease tissues and mucus cell and melanophores hyperplasia in skin of the infected fish. © 2012 Knowledgia Review, Malaysia. Source

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