Animal Health Research Institute AHRI

Al Jīzah, Egypt

Animal Health Research Institute AHRI

Al Jīzah, Egypt
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Mohamed A.G.,Animal Health Research Institute AHRI | Ramadan K.M.,Animal Health Research Institute AHRI | Monem H.A.,Animal Health Research Institute AHRI | Toukhy Essam E.L.,Animal Health Research Institute AHRI | Khairy E.A.,National Research Center of Egypt
Global Veterinaria | Year: 2013

This study was carried out on 55 animals (5 vaccinated cattle with S19 vaccine, 5 vaccinated cattle with RB51 vaccine, 5 vaccinated Sheep with Riv 1 vaccine, 20 cattle from suspected to be infected or had a history of brucellosis and 20 sheep from mobile flocks of known history of brucellosis). The results of serological tests which were applied on serum samples, revealed out of 5/5 vaccinated cattle with S19(100%), (100%) and (100.0%) were positive by RBPT, TAT and Riv.T respectively so vaccinated animals tend to yield persistent post-vaccinal immune responses. While out of 5/5 vaccinated cattle with RB51 were negative by the same serological tests as RB51 a rough strain of Brucella can not detected by these tests. But out of 20 cattle from infected farm with Brucella 5/20(25%), 5/20(25%) and 5/20(25%) were positive by RBPT, TAT and Riv.T respectively. Out of 5 vaccinated Sheep with Riv 1 vaccine 5/5(100%), 5/5(100%) and 5/5(100%) were positive by RBPT, TAT and Riv.T respectively while out of 20 sheep from infected mobile flocks 4/20 (20%), 4/20(20%) and 4/20(20%) were positive by RBPT, TAT and Riv.T respectively. Out of 5 milk samples were collected from seropositive naturally infected cattle and 4 milk samples from seropositive naturally infected sheep were positive PCR amplifies, 731 bp fragment indicated (B. melitensis biovar 3), which considered the most prevalent strain in Egypt. AMOS PCR results were recorded by electrophoretic mobilitis in agarose gels and photographic methods showed two products (498 and 364 bp) plus 178-bp were amplified from RB51 vaccinated cattle but only one product (498 bp) was amplified from the S19 vaccinated cattle. Amplified fragments obtained from vaccinated sheep with Rev 1 vaccine produce 2 bands, 282 bp and 238 bp, the product obtained from digestion of the amplified fragment. In contrast naturally infected sheep with B.melitensis field isolate (B. melitensis biovar3) produced only one band a 238-bp fragment. AMOS PCR was shown to be a valuable tool and a rapid screening test for differentiating the vaccinated animals with S19, RB51 or Riv 1 from infected one with B. melitensis biovar 3(field strains of Brucella) which means that Brucellosis eradication program could reliably use the Brucella AMOS PCR without any supplement to other diagnostic and epidemiological data to release sale animals from quarantine before the conventional identification methods are completed. © IDOSI Publications, 2013.


Wahba N.M.,Animal Health Research Institute AHRI | El Nisr N.A.,Animal Health Research Institute AHRI | Sayed S.M.,Animal Health Research Institute AHRI | Ellah M.A.,Assiut University | And 2 more authors.
Journal of Animal and Veterinary Advances | Year: 2011

The objective of this study was to determine the most suitable and effective regimen of intramammary honey infusion in bovine subclinical mastitis. A total of twenty five subclinical mastitic cows from two farms were divided into two groups; group a: 10 lactating cows were infused 10 mL fennel honey solution 10% in sterile saline/quarter daily for three successive doses; group b: 15 lactating cows were infused day by day for three successive doses guarded with intramuscular antihistaminic drug. Milk samples of both groups showed a decrease in total bacterial count at the 3rd and 10th day and the reduction percent was 99.6 and 99.8%, respectively. Milk cytological results showed a significant increase (p<0.05) in lymphocyte and significant (p<0.05) decrease in neutrophil percentages at the 3rd day up to the 10th day in group b while the same results were achieved at the 10th day in group a. Milk yield records in group a showed decrease by the 3rd day post intramammary infusion and then gradually increased up to the 10th day reaching nearly the same levels. Milk yield of group b showed a slight decrease by the 3rd day and gradually increased to level higher than that before infusion. Haematological study revealed that the percentage of blood neutrophil significantly decreased (p<0.05) from the 3rd up to the 10th day in both groups while the lymphocytic percentage increased significantly (p<0.05). In group a, the eosinophil cells' percentage was significantly increased (p<0.05) at the 3rd day. It was concluded that intramammary 10% honey infusion day by day for three doses guarded by antihistaminic drug could treat bovine subclinical mastitis. Moreover, milk cytology could be used instead of California mastitis test during and after treatment with honey. © Medwell Journals, 2011.


Ali M.R.K.,Georgia Institute of Technology | Ibrahim I.M.,Cairo University | Ali H.R.,Cairo University | Ali H.R.,Animal Health Research Institute AHRI | And 3 more authors.
International Journal of Nanomedicine | Year: 2016

Plasmonic photothermal therapy (PPTT) is a cancer therapy in which gold nanorods are injected at the site of a tumor before near-infrared light is transiently applied to the tumor causing localized cell death. Previously, PPTT studies have been carried out on xenograft mice models. Herein, we report a study showing the feasibility of PPTT as applied to natural tumors in the mammary glands of dogs and cats, which more realistically represent their human equivalents at the molecular level. We optimized a regime of three low PPTT doses at 2-week intervals that ablated tumors mainly via apoptosis in 13 natural mammary gland tumors from seven animals. Histopathology, X-ray, blood profiles, and comprehensive examinations were used for both the diagnosis and the evaluation of tumor statuses before and after treatment. Histopathology results showed an obvious reduction in the cancer grade shortly after the first treatment and a complete regression after the third treatment. Blood tests showed no obvious change in liver and kidney functions. Similarly, X-ray diffraction showed no metastasis after 1 year of treatment. In conclusion, our study suggests the feasibility of applying the gold nanorods-PPTT on natural tumors in dogs and cats without any relapse or toxicity effects after 1 year of treatment. © 2016 Ali et al.


Saleh S.K.,Camel and Range Research Center | Saleh S.K.,Animal Health Research Institute AHRI | Al-Ramadhan G.,Camel and Range Research Center | Faye B.,Camel and Range Research Center | Faye B.,CIRAD - Agricultural Research for Development
Emirates Journal of Food and Agriculture | Year: 2013

Somatic cell counts and bacteriological examinations were measured in 28 camel milk samples from 2 farms, one with milking machine (farm A) and the second with hand milking (farm B). The milk was analyzed for 6 months after the parturition, every month, the first one occurring one week approximately after delivery. The somatic cell count was higher at the first sampling in the two farms but significantly more in farm B. The microbiological contamination was also higher in farm B (37% samples were contaminated) than in farm A (12%). The somatic cell count decreased all along the lactation stage and increased with the parity but the trends were not significant due to the high variability of the values. On average, the normal level of somatic cell counts is low compared to cow.


Ali H.R.,Georgia Institute of Technology | Ali H.R.,Animal Health Research Institute AHRI | Ali H.R.,Cairo University | Ali M.R.K.,Georgia Institute of Technology | And 6 more authors.
Bioconjugate Chemistry | Year: 2016

TB remains a challenging disease to control worldwide. Nanoparticles have been used as drug carriers to deliver high concentrations of antibiotics directly to the site of infection, reducing the duration of treatment along with any side effects of off-target toxicities after systemic exposure to the antibiotics. Herein we have developed a drug delivery platform where gold nanorods (AuNRs) are conjugated to rifampicin (RF), which is released after uptake into macrophage cells (RAW264.7). Due to the nature of the macrophage cells, the nanoparticles are actively internalized into macrophages and release RF after uptake, under the safety frame of the host cells (macrophage). AuNRs without RF conjugation exhibit obvious antimicrobial activity. Therefore, AuNRs could be a promising antimycobacterial agent and an effective delivery vehicle for the antituberculosis drug Rifampicin for use in tuberculosis therapy. © 2016 American Chemical Society.

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