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Maisons-Alfort, France

Nakamura K.,Japanese National Institute of Animal Health | Nakamura K.,Obihiro University of Agriculture and Veterinary Medicine | Mase M.,Japanese National Institute of Animal Health | Yamamoto Y.,Japanese National Institute of Animal Health | And 12 more authors.
Avian Diseases | Year: 2011

From January 2009 to June 2010, many broiler chicks suddenly died without clinical signs. The mortality rates were from 1.2% to 17.0% in affected flocks. Inclusion body hepatitis (IBH) was detected in 13 prefectures (northern, eastern, western, and southern areas) in Japan. The livers were enlarged and pale. The bursa of Fabricius and thymus had not atrophied. Multifocal necroses of hepatocytes with basophilic intranuclear inclusions were seen in the liver. Eosinophilic intranuclear inclusion bodies in hepatocytes were rare. Focal necrosis of acinar cells with basophilic intranuclear inclusions was found in the pancreas. Basophilic intranuclear inclusion bodies were detected in intact surface epithelial cells of gizzard and epithelial cells of the small intestine. The intranuclear inclusions of liver, pancreas, gizzard, and small intestine were stained positively for immunohistochemistry of fowl adenovirus (FAV) antigen. Ultrastructurally, basophilic intranuclear inclusions consisted of viral particles approximately 70 nm in diameter and arranged in a crystalline array. FAV was isolated from the liver of chickens affected with IBH. The serotype of most isolates was 2. This study suggests that IBH produced by FAV is epidemic in broiler chicks in Japan and that the present cases occurred as the primary disease without the association of infectious bursal disease virus or chicken anemia virus. © 2011 American Association of Avian Pathologists.

Goodyear S.N.,Dalhousie University | Peck B.,Animal Health Laboratory
Acta Horticulturae | Year: 2015

Art and the garden have been intertwined and informing each other since the dawn of history. We are interested in the experience of horticulture through the lens of art particularly in horticultural therapy practice. We have conducted a series of workshops with diverse clients, and this paper presents and discusses their response through workshop evaluations. We conclude with reflections on future directions to more fully engage art and horticultural therapy.

Anita A.,University of Agricultural Sciences and Veterinary Medicine, Iasi | Gorgan L.,Al. I. Cuza University | Anita D.,University of Agricultural Sciences and Veterinary Medicine, Iasi | Oslobanu L.,University of Agricultural Sciences and Veterinary Medicine, Iasi | And 2 more authors.
International Journal of Infectious Diseases | Year: 2014

Objective: Swine hepatitis E virus (HEV) is considered to be a new zoonotic agent due to its close genomic resemblance to the human HEV. The aim of this study was to determine human HEV seroprevalence in eastern Romania and to characterize circulating swine HEV sequences. Methods: Serological investigations of human serum samples were done using a commercial ELISA kit (MP Biomedicals). Swine faecal samples were tested to detect the HEV ORF2 sequence by nested reverse transcription PCR. Results: One hundred and forty-eight human serum samples were tested for anti-HEV IgG of which 22 were found to be positive. Fresh swine faeces (pools) were collected from five farms in eastern Romania. Six out of 19 pooled samples were positive for HEV RNA. Phylogenetic analysis based on alignment of the ORF2 sequence indicated that the Romanian swine HEV isolates belonged to genotype 3. Conclusions: This is the first study showing HEV to be present in Romanian pig herds and that the human population is exposed. © 2014 The Authors.

Yang Y.,Jiang Nan University | Cai Y.N.,Jilin Agricultural University | Tong M.W.,Chinese Academy of Agricultural Sciences | Sun N.,Chinese Academy of Agricultural Sciences | And 7 more authors.
One Health | Year: 2016

Trichinellosis is a serious foodborne zoonotic disease. It is an important threat to public health in both developing and developed countries. Human infections are strongly associated with consuming undercooked meat containing infective Trichinella larvae. The development of serological tools has enabled seroepidemiological studies and contributed to our knowledge on the importance of this parasite. Serological tests can also help the diagnosis of parasite infections in humans and the surveillance of animals. Generally speaking, serological techniques include detection methods for specific antibodies and for circulating parasite antigens in the serum or tissue fluids. Here, we present a comprehensive review of various methods used in the detection of antibodies against Trichinella and circulating parasite antigens in animals and humans. © 2016.

Gulson B.,Macquarie University | Gulson B.,CSIRO | Korsch M.,CSIRO | Winchester W.,Esperance Cleanup and Recovery Project | And 10 more authors.
Environmental Research | Year: 2012

In late 2006, the seaside community in Esperance Western Australia was alerted to thousands of native bird species dying. The source of the lead (Pb) was determined by Pb isotopes to derive from the handling of Pb carbonate concentrate through the Port, which began in July 2005. Concern was expressed for the impact of this on the community. Our objectives were to employ Pb isotope ratios to evaluate the source of Pb in environmental samples for use in legal proceedings, and for use in remediation and monitoring. Isotope measurements were undertaken of bird livers, plants, drinking water, soil, harbour sediments, air, bulk ceiling dust, gutter sludge, surface swabs and blood. The unique lead isotopic signature of the contaminating Pb carbonate enabled diagnostic apportionment of lead in samples. Apart from some soil and water samples, the proportion of contaminating Pb was >95% in the environmental samples. Lead isotopes were critical in resolving legal proceedings, are being used in the remediation of premises, were used in monitoring of workers involved in the decontamination of the storage facility, and monitoring transport of the concentrate through another port facility. Air samples show the continued presence of contaminant Pb, more than one year after shipping of concentrate ceased, probably arising from dust resuspension. Brief details of the comprehensive testing and cleanup of the Esperance community are provided along with the role of the Community. Lead isotopic analyses can provide significant benefits to regulatory agencies, interested parties, and the community where the signature is able to be characterised with a high degree of certainty. © 2011.

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