Kandil O.M.,National Research Center of Egypt |
Abdelrahman K.A.,National Research Center of Egypt |
Fahmy H.A.,Animal Health Institute AHRI |
Mahmoud M.S.,National Research Center of Egypt |
And 2 more authors.
Journal of Helminthology | Year: 2016
Haemonchus contortus is a major parasite of small ruminants and its blood-feeding behaviour causes effects ranging from mild anaemia to death. Knowledge of the genetic variation within and among H. contortus populations can provide the foundation for understanding transmission patterns and aid in the control of haemonchosis. Adult male H. contortus were collected from three geographical regions in Egypt. The second internal transcribed spacer (ITS2) of nuclear ribosomal DNA was amplified using the polymerase chain reaction (PCR) and sequenced directly. The population genetic diversity and sequence variations were determined. Nucleotide sequence analyses revealed one genotype (ITS2) in all worms, without genetic differentiation. The similarity in population genetic diversity and genetic patterns observed among the three geographical regions could be attributed to possible movement between the sites. This is the first study of genetic variation in H. contortus in Egypt. The present results could have implications for the rapid characterization of H. contortus and other trichostrongyloid nematodes, and evaluation of the epidemiology of H. contortus in Egypt. Copyright © Cambridge University Press 2016 This is a work of the U.S. Government and is not subject to copyright protection in the United States.