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Pirvu C.D.,Carol Davila University of Medicine and Pharmacy | Ortan A.,University of Bucharest | Hirjau M.,Carol Davila University of Medicine and Pharmacy | Prisada R.,Carol Davila University of Medicine and Pharmacy | And 2 more authors.
Romanian Biotechnological Letters | Year: 2011

In recent years, a considerable effort was dedicated to researching pentoxifylline, because of its effect on the hemorheology, increasing deformability, decreasing the aggregation trend of the red blood cells and the fibrinogen concentration. All of these properties turned pentoxifylline into a drug eligible to be used in peripheral vascular disorders. At the same time, the pharmacological profile of pentoxifylline and its short half-life make it a prime candidate for processing it in encapsulating drugs. This study was aimed at the development and optimization of the encapsulation of pentoxifylline in biodegradable, biocompatible and non-toxic carriers (microspheres, liposomes), in order to improve its bioavailability. Encapsulation of the drug in vesicular structures is recommended for prolonging its existence in the systemic circulation and for reducing its toxicity. Microspheres were prepared by the emulsification of a gelatin solution in an oil phase. In this study, the physicochemical characteristics of the gelatin microspheres were studied (particle size, particle density, crystalline form, drug content and stability). Liposomes were prepared by a physical dispersion method using different ratios of lipids. We have studied the changes that occurred in the entrapment efficiency, particle size and drug stability when different formulation parameters were modified. The physicochemical properties of both encapsulated forms were significantly affected by the formulation parameters. © 2011 University of Bucharest. Source

Ivana S.,University of Agronomical Sciences and Veterinary Medicine | Campeanu G.,University of Agronomical Sciences and Veterinary Medicine | Bogdan A.T.,Animal Genetic Resources Academy David Davidescu Romanian Academy | Manolescu N.,Polytechnic University of Bucharest | And 5 more authors.
Romanian Biotechnological Letters | Year: 2010

Objective: In this work, cats kept as pets from Bucharest were studied with suspect to their contamination with Pasteurella. All the cats that made the object of the research were assessed at the University Hospital of the Faculty of Veterinary Medicine, Bucharest. Design: Case-control study Animals: 44 cats Procedures: The genomic material (total DNA) was digested with 40 U ApaI and 30 SalI, the digestion conditions indicated by the producer (Roche Diagnostics) being respected. The DNA fragments which resulted were separated by a CHEP Mapper (Bio Rad) system in 1% agarose SeaKem Gold gel. The profiles of bands which resulted were interpreted by means of the Fingerprinting II (BioRad) programme. The analysis of the clonal characteristics of the isolates being based on the Dice coefficient and the UPGMA method. Results: From the 44 samples investigated, taken from the oral cavity of cats, 22 Pasteurella strains were isolated. The following species were isolated: P. multocida (18 strains), P. stomatis (1 strain) and P. speciae B (3 strains). The comparison of the profiles of PFGE bands by means of Fingerprint II programme and thir interpretation on the basis of the Dice coefficient and of the UPGMA analysis method revealed a heterogeneous P. multocida population. Conclusions and Clinical Relevance: The Pasteurella species was identified both in clinically healthy and in infected cats. The typing of bacterial isolates justifies its utility especially in the monitoring of their zoonotic pathogenic agent, aimed at an optimum control of the transmission to the human host. © 2010 University of Bucharest. Source

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