Animal Experimentation Unit

São José dos Campos, Brazil

Animal Experimentation Unit

São José dos Campos, Brazil

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Asare G.A.,University of Ghana | Bugyei K.,University of Ghana | Fiawoyi I.,University of Ghana | Asiedu-Gyekye I.J.,University of Ghana | And 4 more authors.
Pharmaceutical Biology | Year: 2013

Context: Phyllanthus niruri L. (Euphorbiaceae), a medicinal plant traditionally known for dissolving kidney stones, is used prophylactically as an antimalarial agent. Objective: The study was undertaken to determine its effect on some male hormones and other toxicological properties due to paucity of its data despite its wide use. Material and methods: Male Sprague-Dawley rats (100-140g) were used. Group 1 [control group (C), n=6] received water. Group 2 [low-dose test group (LD), n=6] received 50mg/kgbody weight (b.wt.) aqueous leaf extract orally. Group 3 [high-dose test group (HD), n=6] received 500mg/kgb.wt. extract for 90 days. Upon sacrifice, among other organs the testes were harvested. Blood samples drawn were used for biochemical (including progesterone, estrogen and testosterone), cytotoxicity and hematological assays. Results: C, LD and HD estrogen values were 192±25, 385±122 and 962±357pg/ml, respectively. In the same order, progesterone values were 96±24, 155±45 and 320±80pg/ml, respectively. Testosterone levels were 5210±1090, 4710±220 and 4500±580pg/ml, respectively. Significant differences were observed in the estrogen and progesterone levels (p=0.001). Degenerative changes were observed histologically. Cytotoxicity at 50% (CC50) was 10.0g/ml. Discussion and conclusion: This antimalarial plant is mildly cytotoxic with male antifertility properties. © 2013 Informa Healthcare USA, Inc. All rights reserved: reproduction in whole or part not permitted.


da Silva R.M.,Animal Experimentation Unit | da Silva R.M.,Hokkaido University | Della Noce B.,Animal Experimentation Unit | Fernanda Waltero C.,Animal Experimentation Unit | And 11 more authors.
International Journal of Molecular Sciences | Year: 2015

In this work we evaluated several genes involved in gluconeogenesis, glycolysis and glycogen metabolism, the major pathways for carbohydrate catabolism and anabolism, in the BME26 Rhipicephalus microplus embryonic cell line. Genetic and catalytic control of the genes and enzymes associated with these pathways are modulated by alterations in energy resource availability (primarily glucose). BME26 cells in media were investigated using three different glucose concentrations, and changes in the transcription levels of target genes in response to carbohydrate utilization were assessed. The results indicate that several genes, such as glycogen synthase (GS), glycogen synthase kinase 3 (GSK3), phosphoenolpyruvate carboxykinase (PEPCK), and glucose-6 phosphatase (GP) displayed mutual regulation in response to glucose treatment. Surprisingly, the transcription of gluconeogenic enzymes was found to increase alongside that of glycolytic enzymes, especially pyruvate kinase, with high glucose treatment. In addition, RNAi data from this study revealed that the transcription of gluconeogenic genes in BME26 cells is controlled by GSK-3. Collectively, these results improve our understanding of how glucose metabolism is regulated at the genetic level in tick cells. © 2015 by the authors; licensee MDPI, Basel, Switzerland.


Saramago L.,Federal University of Rio de Janeiro | Franceschi M.,Federal University of Rio Grande do Sul | Logullo C.,Animal Experimentation Unit | Masuda A.,Federal University of Rio Grande do Sul | And 3 more authors.
International Journal of Molecular Sciences | Year: 2012

In the present work, we produced two monoclonal antibodies (BrBm37 and BrBm38) and tested their action against the triosephosphate isomerase of Rhipicephalus (Boophilus) microplus (RmTIM). These antibodies recognize epitopes on both the native and recombinant forms of the protein. rRmTIM inhibition by BrBm37 was up to 85% whereas that of BrBrm38 was 98%, depending on the antibody-enzyme ratio. RmTIM activity was lower in ovarian, gut, and fat body tissue extracts treated with BrBm37 or BrBm38 mAbs. The proliferation of the embryonic tick cell line (BME26) was inhibited by BrBm37 and BrBm38 mAbs. In summary, the results reveal that it is possible to interfere with the RmTIM function using antibodies, even in intact cells.

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