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Balbinotto R.P.,Clinics Hospital of Porto Alegre HCPA | Trindade M.R.M.,UFRS | Muller A.L.L.,HCPA | Nunes A.G.,UFRS | And 3 more authors.
Acta Cirurgica Brasileira | Year: 2010

Purpose: To verify the frequency of postsurgical pelvic adhesion formation in an experimental animal model using videolaparoscopy. Methods: Experimental study in a sample of 11 non-pregnant female rabbits, aged 5 to 7 months. After general anesthesia, access to the abdominal cavity was performed by an open puncture technique, with 10mm optics, placing two other 5 mm trochars under direct visualization, in the iliac fossae. Then a fragment of peritoneum was resected, followed by electrocauterization. In 21 days, the videolaparoscopy was repeated, and adhesion formation and score was looked at, with biopsies at the surgical site. Results: 54 % of adhesion formation was observed, and the median score of adhesions was 6 (minimum of 3 and maximum of 10), all of them found in the bladder and the anterior abdominal wall. Conclusion: The method used presents a high frequency of intra-abdominal adhesion formation. Source

Balbinotto R.P.,Hospital de Clinicas de Porto Alegre HCPA | Trindade M.R.M.,UFRS | Meyer F.S.,Animal Experimentation Unit Research | Muller A.L.L.,Hospital de Clinicas de Porto Alegre HCPA | And 2 more authors.
Acta Cirurgica Brasileira | Year: 2010

Purpose: To describe the anesthetic protocol and the intubation technique without visualizing the trachea in rabbits, in order to enable the videolaparoscopic surgical procedure. Methods: The experiment was performed on 33 female rabbits (Oryctolagus cuniculus), aged from 5 to 7 months. It consisted of general anesthesia and endotracheal intubation by manual palpation of the trachea of the rabbits, without using the laryngoscope, orally, for later videolaparoscopic surgical access to the abdominal cavity. Results: The mean values and standard deviation of vital parameters of the animals were 223.8±15.61 beats per minute for heart rate; 35±9 movements per minute for respiratory rate; 96.94±0.99% of oxymetry and 42.82±4.02 mmHg for capnometry; 16.7±4.3 minutes for pneumoperitoneum (duration of surgery) and 1 hour and 14±8.52 minutes for time of observation (from induction to recovery from anesthesia). All animals were intubated in at most three attempts. No animals were lost after the introduction of this anesthetic technique. Conclusion: This protocol proved adequate, safe and easy to perform, on rabbits submitted to videolaparoscopic surgery. Source

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