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Messarah M.,Annaba University | Amamra W.,Annaba University | Boumendjel A.,Annaba University | Barkat L.,Annaba University | And 4 more authors.
Toxicology and Industrial Health | Year: 2013

The aim of this study was to evaluate the protective effects of vitamin E and/or curcumin against diazinon (DZN) (an organophosphorus insecticide)-induced toxicity of blood, liver and erythrocyte markers of male Wistar rats. The exposure of rats to DZN for 21 days provoked significant changes in red blood cell counts and hemoglobin. Results showed that lipid peroxidation increased significantly in DZN-treated rats, as evidenced by high liver and erythrocyte thiobarbituric acid reactive substance levels. Alteration of the antioxidant system in DZN-treated rats was confirmed by the significant decrease in the activity of catalase, glutathione peroxidase and glutathione-S-transferase, accompanied by a decline in reduced glutathione content in both tissues. On the other hand, a significant increase in the activities of plasma aspartate transaminase, alanine transaminase, lactate dehydrogenase and alkaline phosphatase was observed in the rats treated with DZN. However, the administration of vitamin E and curcumin has ameliorated the previous markers. In conclusion, our results indicate that the natural antioxidants like vitamin E and curcumin can effectively lower the erythrocytes and hepatic injuries induced by DZN as monitored by lipid peroxides, antioxidant enzyme activities and sensitive serum enzyme levels. © The Author(s) 2012. Source


Samira J.,CNRS Chemistry Institute of Rennes | Samira J.,Animal Ecophysiology Laboratory | Hassane O.,CNRS Chemistry Institute of Rennes | Mongi S.,Animal Ecophysiology Laboratory | And 5 more authors.
Biotechnology Letters | Year: 2014

The performance therapy of chitosan (CH)-doped bioactive glass (BG) has been evaluated in vitro and in vivo. In vitro, the effect of CH-BG was assessed on human Saos-2 osteoblast cells. In vivo, Wistar rats were ovariectomized (OVX) and CH, BG and CH-BG were implanted in bone tissue. After 3 days of CH-BG contact, cell viability of Saos-2 osteoblast increased by 16.4 % as compared to the control group. The runt-related transcription factor 2 (RUNX2/Cbfa1) and osteocalcin (OC) gene expressions were significantly increased with 600 and 300 %, respectively, in contact of CH-BG as compared with CH. In vivo, the apoptotic index in the OVX-CH-BG group was decreased by 80 %. A mechanical hardness test showed a significant bone strength improvement after CH-BG implantation (40 %). The CH-BG composite may therefore prove clinically useful as a bioactive bone substitute. © 2014, Springer Science+Business Media Dordrecht. Source


Jebahi S.,CNRS Chemistry Institute of Rennes | Jebahi S.,Animal Ecophysiology Laboratory | Oudadesse H.,CNRS Chemistry Institute of Rennes | Keskes H.,University of Sfax | And 4 more authors.
Journal of the Korean Society for Applied Biological Chemistry | Year: 2013

Strontium (Sr) compounds have become increasingly popular in the field of osteoporosis treatment. However, the quality of new bone after implantation of strontium-containing bioceramics has yet to be investigated. In the present study, the newly formed bone tissue around strontium-doped bioactive glass (BG-Sr) implants was characterized. BG-Sr was implanted in the femoral condyl of ovariectomised rats (OVX). The resected bone was prepared for analysis using several physico-chemical and biological assays such as Fourier transform infrared spectroscopy, X-ray diffraction, scanning electron microscopy, energy-dispersive X-ray, and histomorphometry. BG-Sr biomaterial favored calcium phosphate layer integration on the surface of the glass and offered better bioactivity. Moreover, the histomorphometric analysis demonstrated that BV/TV, N. Ob were significantly higher in BG-Sr treated rats groups than those of BG groups. However, Ob. S/BS, and OV/BV were significantly lower in BG-Sr treated rats groups than those of BG groups. The (Oc.S/BS) was significantly decreased in BG-Sr groups, when compared with that of BG rat groups. On the other hand, the MS/BS had not significantly decreased in the BG-Sr treated rats groups when compared with that of BG groups, however; it was significantly higher when compared with control and OVX groups. These findings suggest that BG-Sr can be used as an inhibitory therapeutic potential of osteoporosis by delivering strontium to stimulate new bone remodeling. © 2013 The Korean Society for Applied Biological Chemistry. Source

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