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Zheng P.,Sichuan Agricultural University | Zheng P.,Animal Disease Resistance Nutrition Key Laboratory of Sichuan Province | Yu B.,Sichuan Agricultural University | Yu B.,Animal Disease Resistance Nutrition Key Laboratory of Sichuan Province | And 12 more authors.
British Journal of Nutrition | Year: 2013

Oxidative stress is detrimental to animals. Previous studies have indicated that arginine (Arg) may function as a potential substance against oxidative stress. The present study was conducted to explore the potential mechanisms behind the Arg-induced protective effects against oxidative stress in piglets. A total of thirty-six piglets were randomly allocated to six groups with six replicates per group. Piglets were subjected to three dietary treatments (namely two groups per treatment) in week 1 and fed with a basal diet (ArgL) or the basal diet supplemented with 0·8 % (ArgM) or 1·6 % (ArgH) l-Arg, respectively. On day 8, piglets were injected intraperitoneally either with diquat (10 mg/kg body weight) or sterile saline. The whole trial lasted 11 d. Results showed that dietary Arg supplementation did not affect growth performance in week 1. Oxidative stress significantly decreased the growth performance of piglets (P< 0·05). However, ArgH attenuated the negative effects of oxidative stress on feed intake and significantly increased the total antioxidant capacity in the liver under oxidative stress (P< 0·05). Both ArgM and ArgH enhanced the activities of plasma glutathione peroxidases and superoxide dismutases and decreased the IL-6 and TNF-α mRNA level in the liver under oxidative stress (P< 0·05). The present study not only shows that Arg can function as a potential nutrient to alleviate oxidative stress responses through the enhancement of antioxidant capacity, and inhibition of the expression of inflammatory cytokines, but the results also suggest that alleviation of oxidative stress responses using dietary nutrient components deserves further attention in the future. © 2012 The Authors.


Chen Y.,Sichuan Agricultural University | Chen Y.,Animal Disease Resistance Nutrition Key Laboratory of Sichuan Province | Chen D.,Sichuan Agricultural University | Chen D.,Animal Disease Resistance Nutrition Key Laboratory of Sichuan Province | And 10 more authors.
British Journal of Nutrition | Year: 2012

The present study evaluated whether dietary arginine (Arg) supplementation could attenuate immune challenge induced by Salmonella enterica serovar Choleraesuis C500 (S.C500) through the Toll-like receptor (TLR) 4-myeloid differentiation factor 88 (Myd88) signalling pathway in weaned piglets. A total of thirty-six weaned pigs were randomly allocated into six groups with six replicates per group. Pigs were subjected to three dietary treatments (namely two groups per treatment) in the first week (0-7d) and fed with diets containing 0, 0.5 and 1.0% l-Arg, respectively. On day 8, pigs were injected intramuscularly either with S.C500 or sterile saline. Serum samples were collected at day 8 (before injection), and at 1, 3 and 10d post-injection, pigs were killed for evaluation of tissue gene expression following the last blood collection. Piglets fed the diets with 0.5 or 1.0% Arg supplementation had a higher concentration of serum Arg (P<0.05). S.C500-challenged piglets had higher (P<0.05) serum antibody levels during the days 9-18. Weight gain and feed intake were decreased remarkably (P<0.01) after the injection of S.C500, and 0.5 or 1.0% Arg supplementation tended to alleviate the inhibition. The S.C500 challenge significantly enhanced (P<0.05) serum C-reactive protein (CRP), interferon-γ and IL-12 concentrations, but Arg supplementation attenuated (P<0.05) the increase in CRP level. The mRNA expression of TLR4, TLR5, Myd88, p65 NF-κB and TNF-α was up-regulated (P<0.05) by the S.C500 challenge in different tissues, but was down-regulated (P<0.05) by Arg supplementation. In conclusion, Arg supplementation could inhibit the excessive activation of the TLR4-Myd88 signalling pathway and thus attenuated the negative effects caused by the immune challenge of S.C500. © 2011 The Authors.

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