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Liu Q.,Academy of Military Medical Science | Cao L.,Jilin Academy of Animal Husbandry and Veterinary Medicine Changchun | Zhang Y.,Academy of Military Medical Science | Xu D.,Academy of Military Medical Science | And 7 more authors.
Vector-Borne and Zoonotic Diseases | Year: 2013

Echinococcus granulosus is the causative agent of cystic echinococcosis with medical and veterinary importance worldwide. The aim of this study was to determine the genotype distribution of E. granulosus in animals in Yushu, Qinghai Province, northeastern China. The mitochondrial nicotinamide adenine dinucleotide+hydrogen (NADH) dehydrogenase subunit 1 (nad1) and cytochrome c oxidase subunit 1 (cox1) genes from 30 echinococcosis isolates were analyzed by sequence alignment, generating two unique sequence profiles at both nad1 and cox1 loci. Phylogenetic analysis demonstrated that 28 isolates (93.3%) belonged to the well-known G1-G3 complex (E. granulosus sensu stricto), and 2 (6.7%) were placed in G6-G10 complex (E. canadensis). The present study provides the genetic composition of E. granulosus from animals in Yushu, Qinghai Province, and confirms that, for the first time, the E. granulosus G6-G10 complex (E. canadensis) is not only limited to Xinjiang and might be of greater public health significance than previously believed in China. © Copyright 2013, Mary Ann Liebert, Inc. 2013. Source


Xulong L.,Institute of Military Veterinary | Hailong Q.,Institute of Military Veterinary | Zhaoyang B.,Institute of Military Veterinary | Yanling Y.,Institute of Military Veterinary | And 7 more authors.
Tropical Animal Health and Production | Year: 2011

The seroprevalence of Brucella infection in yaks was surveyed on the Qinghai-Tibet plateau of China in 2010. A total of 621 serum samples was collected from six counties and were tested by serum agglutination test. The results showed that 56 (9%) of the samples were positive for Brucella. The results of the present investigation indicate that brucellosis is common in yaks on the Qinghai-Tibet plateau of China. © 2010 Springer Science+Business Media B.V. Source


Li Y.,Academy of Military Medical Science | Teng L.,Academy of Military Medical Science | Shang L.,Academy of Military Medical Science | Ma R.,Animal Disease Control and Prevention Center in Qinghai Province | And 5 more authors.
Journal of Animal and Veterinary Advances | Year: 2013

The present study was conducted to investigate tick species and tick-transmitted Theileria and Babesia species in Qinghai Province, Northwestern China. A total of 357 ticks, belonging to 2 Ixodid genera, Haemaphysalis qinghaiensis (167; 46.8%) and Dermacentor nuttalli (190; 53.2%) were collected from the domestic animals during May and June 2010. The ticks were separated into 78 tick pools according to their species and examined for Theileria and Babesia species by a nested PCR targeting 18S rRNA gene. Of the 25 H. qinghaiensis pools, 2 (8.0%) were tested positive for Theileria. Only 1 (1.9%) was tested positive for Babesia in 53 D. nuttalli pools. The sequence and phylogenetic analyses showed that Babesia and Theileria detected in this study were grouped into B. caballi and T. luwenshuni, respectively implying the potential existence of fheileriosis and babesiosis in animals in Qinghai Province. © Medwell Journals, 2013. Source


Yu Z.,Chinese Academy of Sciences | Yu Z.,Military Veterinary Research Institute of Academy of Military Medical science | Cheng K.,Military Veterinary Research Institute of Academy of Military Medical science | Cheng K.,Shandong Academy of Agricultural Sciences | And 18 more authors.
Veterinary Microbiology | Year: 2014

Avian influenza viruses (AIVs) are globally important contagions. Several domestic mammals can be infected with AIVs and may play important roles in the adaptation and transmission of these viruses in mammals, although the roles of wild mammals in the natural ecology of AIVs are not yet clear. Here, we performed a serological survey of apparently healthy Plateau pikas at Qinghai Lake in China to assess the prevalence of exposure to AIVs. Ninety-two of 293 (31%) of wild Plateau pikas possessed serum antibodies against a lowly pathogenic avian influenza (LPAI) H9N2 virus. Experimental inoculation of Plateau pikas with a LPAI H9N2 virus resulted in productive viral replication in respiratory tissues without prior adaptation. Our findings suggest that Plateau pikas represent a natural mammalian host to H9N2 AIVs and may play a role in the ongoing circulation of H9N2 viruses at Qinghai Lake in China. Surveillance for AIV infection in Plateau pika populations and other mammals that have close contact with the Plateau pikas should be considered. © 2014 Elsevier B.V. Source


Liu Q.,Academy of Military Medical Science | Cai J.,Animal Disease Control and Prevention Center in Qinghai Province | Zhao Q.,Animal Disease Control and Prevention Center in Qinghai Province | Shang L.,Academy of Military Medical Science | And 6 more authors.
Tropical Animal Health and Production | Year: 2011

The seroprevalence of Toxoplasma gondii infection in yaks (Bos grunniens) was surveyed in Qinghai Province, northwestern China in May and June 2010. A total of 650 serum samples were collected from six counties and assayed for T. gondii antibodies by an indirect hemagglutination test. Antibodies to T. gondii were found in 35.08% (228/650) with the highest rate of 55.34% in Chengduo County. The results of the present survey indicated that infection with T. gondii in cattle is widely spread in China, including yaks in Qinghai Province. © 2011 Springer Science+Business Media B.V. Source

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