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Breeding, Algeria

Arabha Najafabadi H.,University of Tehran | Niasari-Naslaji A.,University of Tehran | Atakpour A.B.,University of Tehran | Ghaffari S.,University of Tehran | And 7 more authors.
Journal of Camel Practice and Research | Year: 2013

Effect of camel preservation milk on SCC and bacteriological isolation was investigated. Samples were collected from individual quarters (n=16) of 5 milking camels. Five minutes after receiving oxytocin (20 I.U), teat was washed and calf was released to stimulate the dam. Next, the suckling was interrupted, the teat was dried with tissue and the sample was collected aseptically for bacterial culture. Subsequently, milk samples were collected for SCC into tube with and without potassium dichromate. Frozen samples were thawed at room temperature and cultured one week after collection. For 4 days after milk samplings, SCC and culture of milk was conducted on a daily basis, maintaining the sample at 4°C. Out of 16 samples, 6 cases were free from any bacterial contamination and in 10 cases single pure bacteria were isolated. The possibility of isolating bacteria from fresh sample on the day of milk collection was greater than frozen specimen (p=0.07). SCC in milk samples without preservative decreased steadily during 4 days storage (P<0.0001); however, such decrease was not noticed in samples with preservative (P>0.05). Freezing adversely affected SCC with or without preservative. In conclusion, preservation of camel milk samples with potassium dichromate over 4 days at 4°C is a valid method to investigate SCC. However, due to the inconsistency in bacteriological isolation following storage at 4°C and freezing, it is advised to perform bacteriological investigation immediately following milk sampling in camel.

Ghaffary S.,University of Tehran | Niasari-Naslaji A.,University of Tehran | Safi S.,University of Tehran | Shirazi-Beheshtiha S.H.,University of Tehran | And 9 more authors.
Journal of Camel Practice and Research | Year: 2015

The objective of the present study was to investigate the possible use of Milk Amyloid A (MAA) as a sensitive biomarker to detect subclinical mastitis in the lactating dromedary camel. Quarter milk samples (n=120), from 65 milking dromedary camels, were collected to evaluate somatic cell count (SCC) and MAA. At the area under the curve of 0.859 for MAA (P<0.001) and cut off points of 306000 cells/ml and 1040 ng/ml for SCC and MAA, respectively. The sensitivity and specificity of the MAA test to detect subclinical mastitis were 100 and 43.9%, respectively. In conclusion, it might be possible to use MAA measurement, as screening test, for early detection of suh-clinical mastitis in dromedary camel.

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