Rajasthan, India
Rajasthan, India

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Kumar J.,Central Sheep and Wool Research Institute CSWRI | Tripathi B.N.,Central Sheep and Wool Research Institute CSWRI | Kumar R.,Animal Biotechnology Section | Sonawane G.G.,Central Sheep and Wool Research Institute CSWRI | Dixit S.K.,Central Sheep and Wool Research Institute CSWRI
Tropical Animal Health and Production | Year: 2013

Corynebacterium pseudotuberculosis, a Gram-positive bacterium is the causative agent of caseous lymphadenitis (CLA), a chronic disease of sheep, goats and other warm blooded animals. In the present study, a total of 1,080 sheep reared under semi-intensive system on organized farms situated in the semi arid tropical region of Rajasthan, India, was clinically examined. Pus samples from superficial lymph nodes of 25 (2.31 %) adult sheep showing clinical lesions similar to CLA were collected for laboratory analyses. On the basis of morphological, cultural and biochemical characteristics 12 (48 %) bacterial isolates from pus identified it as C. pseudotuberculosis. A polymerase chain reaction (PCR) assay targeting Putative oligopeptide/dipeptide ABC transporter, nicotinamide adenine dinucleotide phosphate (NADP) oxidoreductase coenzyme F420-dependent and proline iminopeptidase (PIP) genes of C. pseudotuberculosis was developed that showed 14 pus samples as positive. All C. pseudotuberculosis isolates were also found positive for these genes in the PCR. The specificity of the PCR products was confirmed by sequencing of the amplified products that showed 98-100 % homology with published sequences available in the NCBI database. The present study shows the incidence of CLA as 2.31 %, 1.1 % and 1.29 % based on clinical, bacterial culture and direct pus PCR assay, respectively. The PCR assay was rapid, specific and as significant as bacterial culture in detecting bacteria directly in the clinical pus samples. The PCR assay developed in the study can be applied for the diagnosis and control of CLA. Furthermore, the assay can also be applied to detect C. pseudotuberculosis in various clinical samples. © 2013 Springer Science+Business Media Dordrecht.

Meena A.S.,Central Sheep and Wool Research Institute | Kumar R.,Central Sheep and Wool Research Institute | Kumari R.,Scientist | Jyotsana B.,Central Sheep and Wool Research Institute | And 2 more authors.
Indian Journal of Animal Sciences | Year: 2013

Polymorphism in the melatonin receptor 1A (MTNR1A) is associated with seasonal reproduction of sheep. Genetic polymorphism in MTNR1A gene was investigated in 4 Indian sheep breeds reared in semi-arid and arid region of the Rajasthan. In this study, blood samples were collected from 171 animals. DNA was extracted from white blood cells. The PCR product (824bp) of exon II fragment of sheep MTNR1A gene was amplified. PCR products were digested separately with 2 restriction endonucleases, viz. MnII and RsaI. Polymorphism was detected at both loci of MTNR1A gene. At MnII locus, allele frequency for Magra, Garole, Malpura and Avikalin breed was found as 0.74, 0.74, 0.78, and 0.84 for M allele and 0.26, 0.26, 0.22, 0.16 for N allele, respectively. At RsaI locus, allele frequency was found as 0.90, 0.83, 0.69, and 0.59, for R allele and 0.10, 0.17, 0.31, 0.41 for r allele, respectively. It was concluded that the frequencies of 'M' and 'R' alleles of MTNR1A were higher in Indian sheep breeds.

Jyotsana B.,Animal Biotechnology Section | Jyotsana B.,Central Sheep and Wool Research Institute | Kumar R.,Animal Biotechnology Section | Kumar R.,Central Sheep and Wool Research Institute | And 10 more authors.
Indian Journal of Animal Sciences | Year: 2014

Two genetic variants (A and B) and three genotypes (AB, BB and AA) were found in studied sheep breeds. The average frequency of A and B allele was (0.59) and (0.41) respectively. The A allele was more frequent among the studied breeds except Patanwadi and Kendrapada breeds. There was no clear cut predominance of any of the genotype. The Patanwadi, Malpura, Dumba, Kendrapada and Chokla populations were not in Hardy-Weinberg equilibrium. These results suggested the presence of β-LG gene polymorphism and predominance of β-LG A type in majority of the studied breeds. From the above findings it may be concluded that the native sheep breeds depicts variation in β-LG exon II locus. Further, genotype and allele distribution pattern in different breeds may be due to different characteristic of milk of these breeds. The contrasting pattern of variation observed in present study compared to previous study in native sheep breeds also highlight the need for further studies using large number of animals from different geographic regions. The relationship between β-LG genetic variants and traits related to milk and cheese production characteristics in rest of Indian sheep breeds need to be explored.

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