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Kumar De A.,National Dairy Research Institute | Malakar D.,National Dairy Research Institute | Malakar D.,Animal Biotechnology Center
Indian Journal of Animal Sciences | Year: 2011

The present study was undertaken to produce interspecies embryos between sheep oocytes and goat spermatozoa through simple method of in vitro fertilization. Sheep oocytes were collected from slaughter house derived ovaries and were matured in vitro. Goat semen was collected by artificial vagina method and was processed for removal of seminal plasma and in vitro capacitation. Interspecies embryos were produced by co-incubation of in vitro matured sheep oocytes and in vitro capacitated goat spermatozoa. Total 89 interspecies blastocysts were produced. The study demonstrates a simple method of production of interspecies embryos between sheep and goat.

De S.,Animal Biotechnology Center | Mir N.A.,Dairy Cattle Physiology
International Journal of Dairy Technology | Year: 2014

Nowadays, studies about the anti-obesity potential of probiotics are of growing interest. Lactobacilli are one of the well-studied probiotics owing to their preventing effect on metabolic disorders. This study was undertaken to access the anti-obesity effect of probiotic dahi containing Lactobacillus casei NCDC 19 on C57BL/6 mice. Feeding of probiotic dahi showed reduce body weight gain and epididymal fat weights. Moreover, blood glucose, plasma lipids and expression level of leptin were reduced and caecal bifidobacteria counts and adiponectin expression levels were significantly increased. It can be concluded that feeding of probiotic dahi containing L. casei NCDC 19 showed potential anti-obesity effects. © 2014 Society of Dairy Technology.

Shanmugam M.,Project Directorate on Poultry | Shanmugam M.,National Dairy Research Institute | Pandita S.,DCP Division | Pandita S.,National Dairy Research Institute | And 2 more authors.
Indian Journal of Animal Sciences | Year: 2013

This study examined the effects of S-nitroso-N-acetyl-penicillamine (SNAP), a nitric oxide (NO) donor and Nw-nitro-L-arginine methyl ester hydrochloride (L-NAME), a nitric oxide synthase inhibitor on estradiol-17β and progesterone production by buffalo granulosa cells. Granulosa cells (3×105) from small (≤5 mm diameter) or large (≥ 9 mm diameter) follicles were cultured for 24 h under completely serum-free conditions in DMEM: nutrient mixture F-12 Ham (1:1 ratio) supplemented with 10-7 M androstenedione, 5 mg/ml human apo-transferrin, 0.1% BSA, in the presence or absence of FSH (8 ng/ml). Granulosa cells from large follicles produced higher estradiol-17β and progesterone than those from small follicles. SNAP reduced estradiol-17β and progesterone production at concentrations of 0.1 and 1 mM when used alone and in the presence of FSH by granulosa cells from follicles of both size categories. L-NAME (0.2, 1 and 5 mM) had, however, no effect on estradiol-17β or progesterone production, alone or in the presence of FSH. Our results demonstrated strong inhibitory effects of NO on estradiol-17β and progesterone production by buffalo granulosa cells from small and large follicles and indicated that it may be an autocrine/paracrine regulator of steroidogenesis by granulosa cells in buffalo.

Jain A.,NDRI | Jain T.,Animal Biotechnology Center | Mitra A.,Indian Veterinary Research Institute
Indian Journal of Animal Research | Year: 2012

Ghrelin, a novel motilin related 28-amino acid peptide, is an endogenous ligand for GH secretagogue receptor (GHS-R). In addition to its neuro-endocrine effects in the control of growth hormone (GH) secretion and food intake, recently an unexpected reproductive facet of ghrelin has emerged as its expression and cognate receptors are reported in the rat testis. However, the presence of this signalling system is yet to be ascertained in the buffalo gonads. In this study, amplified 230 bp of ghrelin gene from buffalo corpus luteum (CL) using cDNA as a template have been taken. Also, by immunohistochemistry using specific polyclonal antibodies, the presence and localization of ghrelin in the cyclic and pregnant CL were assessed. Expression of ghrelin protein was persistent local observed in the estrous cycle and early pregnancy (~d40). However, the signals of ghrelin protein were found to be higher during early pregnancy compared to estrous cycle. These dynamic changes in the expression profile of ghrelin during the estrous cycle and throughout the pregnancy propose a precise regulation of ovarian expression of ghrelin, which could portray the potential role of ghrelin in regulation of luteal development. In conclusion our study is the first to demonstrate the expression of ghrelin in the cyclic and pregnant buffalo CL which opens up the possibility of using this molecule in regulating the reproduction.

Jha N.,Anmol Feeds Private Ltd | De S.,Animal Biotechnology Center | Kundu S.S.,National Dairy Research Institute
Indian Journal of Animal Sciences | Year: 2013

In the present study, methanotrophs inhabiting the rumen of Murrah buffalo were detected by molecular technique. The methanotrophs specific primer sets (type l and type ll methanotrophs) were used to amplify 16S rDNA sequence from buffalo rumen. The PCR product of an expected DNA length was observed in the 1% agarose gel, which was purified and then cloned. Different gene clones were screened for inserts using the respective PCR primers. Out of 15 colonies, 10 colonies were positive for inserts of 16S rRNA gene sequences. One recombinant plasmid DNA from each group was sequenced and the nucleotide sequence (592bp and 486bp) obtained with type 1 and type 2 primer sets were deposited in the GenBank database under the accession number HQ699778 and HQ699779, respectively. The sequences obtained in present study showed ≤83% sequence similarity with known methanotrophs and most closely (96 to 99%) related to the 16S rRNA gene of uncultured rumen bacteria. The findings lead to a supposition that the novel methanotrophs with 16S rRNA gene similar to uncultured rumen bacteria are present in the Murrah buffalo rumen.

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