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Su J.,Jiangnan University | Wang H.,Jiangnan University | Ma C.,Jiangnan University | Liu C.,Jiangnan University | And 3 more authors.
European Food Research and Technology | Year: 2015

Effect of gypenosides on porcine pancreatic lipase (PL) activity was determined in vitro. Gypenosides inhibited the PL activity in a dose-dependent manner at the concentrations of 0–1.0 mg/ml. The Lineweaver–Burk plots for gypenosides indicated that the inhibition on PL by gypenosides was noncompetitive. The conformation of PL was investigated by means of circular dichroism and fluorescence. The result revealed the decrease in α-helix contents, increase in β-sheet and the decrease in fluorescence intensity after gypenosides treatment. The incorporation of cholesterol into micelles was inhibited by gypenosides. A complex was observed by transmission electron microscopy, indicating interaction between cholesterol and gypenosides which led to the decrease in cholesterol micelle solubility. The result revealed the fact that gypenosides played a role in inhibiting PL activity and reducing intestinal cholesterol absorption via inhibition of micelle formation. © 2015 Springer-Verlag Berlin Heidelberg Source


Su J.,Jiangnan University | Wang H.,Jiangnan University | Ma C.,Jiangnan University | Lou Z.,Jiangnan University | And 4 more authors.
Food and Function | Year: 2015

This study was conducted to investigate the components of a new resource food in China, peony seed oil (PSO) by GC-MS (gas chromatography-mass spectrometry), its inhibitory effects on carbohydrate hydrolyzing enzymes in vitro and its anti-diabetic effects on mice induced by streptozotocin (STZ). The results showed that peony seed oil showed weak anti-α-amylase activity; however, strong anti-α-glucosidase activity was noted. The GC-MS analysis of the oil showed 9 constituents of which α-linolenic acid was found to be the major component (38.66%), followed by linoleic acid (26.34%) and oleic acid (23.65%). The anti-diabetic potential of peony seed oil was tested in STZ induced diabetic mice. Administration of peony seed oil and glibenclamide reduced the blood glucose level and the area under curve (AUC) in STZ induced diabetic mice. There were significant increases in body weight, liver glycogen content, serum insulin level, high-density lipoprotein cholesterol (HDL-C) and decreases in glycosylated hemoglobin (HbA1C), total serum cholesterol (TC), and triglyceride (TG) in test groups as compared to the untreated diabetic groups. In vivo antioxidant studies on STZ induced diabetic mice revealed the reduction of malondialdehyde (MDA) and increase of glutathione peroxides (GSH-px), superoxide dismutase (SOD), and glutathione (GSH). The results provided a sound rationale for future clinical trials of oral administration of peony seed oil to alleviate postprandial hyperglycemia in streptozotocin-induced diabetic mice. © 2015 Royal Society of Chemistry. Source


Su J.,Jiangnan University | Wang H.,Jiangnan University | Ma C.,Jiangnan University | Liu C.,Jiangnan University | And 3 more authors.
International Journal of Food Sciences and Nutrition | Year: 2016

This study investigated the hypocholesterolaemic effects of bitter melon aqueous extracts (BMAE) in vitro, the inhibitory effects of BMAE on pancreatic cholesterol esterase (CEase) and incorporation of cholesterol into micelles were investigated. BMAE decreased the in vitro micellar solubility of cholesterol in a dose-dependent manner. The conformation of CEase was investigated by means of circular dichroism (CD) and fluorescence. The result revealed the decrease of α-helix contents, increase of β-sheet and exposure of aromatic amino acid residuals. The incorporation of cholesterol into micelles was inhibited by BMAE. A complex was observed by transmission electron microscopy (TEM), which indicated interaction between cholesterol and BMAE. The result revealed that BMAE can play a role in decreased intestinal cholesterol absorption via inhibition of CEase, and of micelle formation. © 2015 Taylor & Francis. Source

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