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Cheng Z.-D.,Anhui Medical University | Hu S.-L.,Anhui Medical University | Sun Y.-B.,Anhui Province Hospital | Xu W.-P.,Anhui Evidence based Medicine Center | And 2 more authors.
Chinese Journal of Cancer | Year: 2010

Background and Objective: Transcriptional silencing induced by CpG island methylation is believed to be one of the important mechanisms of carcinogenesis. Checkpoint with fork head-associated and ring finger (CHFR) governs the transition from prophase to prometaphase in response to mitotic stress. This study was to analyze the relationship between the methylation of CHFR gene and the clinicopathologic features of gastric cancer, and the difference of results between methylation- specific polymerase chain reaction (MSP) and combined bisulfite restriction analysis (COBRA) in detecting aberrant methylation of CHFR gene in gastric cancer. Methods: Both MSP and COBRA methods were used to detect the promoter methylation of CHFR gene in gastric cancer specimens from 64 patients. The relationship between methylation status of CHFR gene and the clinicopathologic features of gastric cancer were analyzed using SPSS16.0. Results: The methylation rates of CHFR gene promoter were significantly higher in gastric cancer samples than in the corresponding paracancer normal gastric mucosa by MSP (51.6% vs. 18.8%, P < 0.001). However, there was no significant correlation between methylation status of CHFR gene and the clinicopathologic parameters of gastric cancer, including age, gender, tumor size, clinical stage, Borrman type, tumor Invasion depth, differentiation, and lymph node metastasis (P > 0.05). Aberrant methylation of the CHFR gene was detected in 27 (42.2%) of the 64 specimens of gastric cancer using COBRA, which did not significantly differ from that using MSP (P > 0.05). Conclusions: Aberrant methylation of the CHFR gene is a frequent event in the carcinogenesis of gastric cancer. Detecting the methylation of CHFR gene In gastric mucosa may conduce to the diagnosis of gastric cancer. No difference was found between MSP and COBRA in detecting promoter methylation of CHFR gene in gastric cancer.

Ma M.,University of North Carolina at Chapel Hill | Ma M.,Anhui Science and Technology University | Wang Y.,University of North Carolina at Chapel Hill | Ru Y.,Anhui Province Hospital | Wang Z.-F.,University of North Carolina at Chapel Hill
Chinese Journal of Biomedical Engineering | Year: 2012

The sequence classification methods have broad application in various bioinformatics areas such as the identification of regulatory elements of transcription and the prediction of protein structure. Here we presented a new classification method to analyze short sequences based on their sequential features, and used this method to study RNA splicing regulatory elements. This method extracted the sequential features from the known spicing regulatory elements, and developed a scoring system to evaluate how possible a given short sequence can regulate RNA splicing. This method was compared with some other methods through applying to a set of exonic splicing enhancer (ESE) and silencer (ESS) octamers. The average prediction accuracy of this sequential feature-based method for three kinds of computation validation experiments reached about 93% and the transparent predictive structure of the method helps to interpret the biological mechanism. This paper shows a new method for biology series' data analysis and presents a new way for the study of regulatory sequences that control gene expression.

Li J.-P.,Anhui University of Traditional Chinese Medicine | Xu W.-P.,Anhui Province Hospital | Li P.-F.,Hefei University of Technology | Hu Y.,Anhui University of Traditional Chinese Medicine | And 2 more authors.
Chinese Journal of New Drugs | Year: 2010

Objective: To prepare Ag/TiO2 nano-materials by photo-reduction for the antibacterial effect, and to optimize the components. Methods: Based on the inhibition growth curves of E. coli, we comprehensively evaluated the antibacterial effects of the nano-materials, and optimize the components. Results: The nano-materials with the ratio of 6.72 μg Ag: 500 μg TiO 2 showed the optimal antibacterial effect. Conclusion: In a series of Ag/TiO2 nano-materials prepared by photo-reduction, the optimized components show a better antibacterial effect than the single components, which may have a potential medicinal application.

Luo L.,Hefei University of Technology | Qin J.-J.,Hefei University of Technology | Sun W.,Hefei University of Technology | Fang X.-D.,CAS Anhui Institute of Optics and Fine Mechanics | Yan Z.-Y.,Anhui Province Hospital
Guangdianzi Jiguang/Journal of Optoelectronics Laser | Year: 2010

Argon ion bombardment(AIB) is used to improve the diamond-like carbon(DLC) films on the mechanical heart valve made from pyrolytic carbon. The DLC film is deposited by the pulsed laser deposition(PLD). The microstructure of the DLC films is investigated by the visible Raman spectroscopy and the X-ray photoelectron spectroscopy(XPS) before and after AIB, respectively; and the surface of DLC film is observed with microscope before and after AIB, respectively. The experimental results demonstrate that the AIB is unable to cause corrugation or desquamation of the DLC films, but it modifies the film microstructure, enhances the ratio of sp3/sp2 at certain extent, dopes a little argon in the film, and decrease oxygen on the film effectively, but it couidn't decrease C-O; C=O and COOH in the films effectively.

Hu S.-L.,Anhui Medical University | Kong X.-Y.,Anhui Medical University | Cheng Z.-D.,Anhui Medical University | Sun Y.-B.,Anhui Province Hospital | And 6 more authors.
Tumori | Year: 2010

Aims and background. Transcriptional silencing induced by hypermethylation of CpG islands in the promoter regions of genes is believed to be an important mechanism of carcinogenesis in human cancers including gastric cancer. A number of reports on methylation of various genes in gastric cancer have been published, but most of these studies focused on cancer tissues or only a single gene. In this study, we determined the promoter hypermethylation status and mRNA expression of 4 genes: p16, Runx3, DAPK and CHFR. Methods. Methylation-specific polymerase chain reaction (MSP) was used to determine the methylation status of p16, Runx3, DAPK and CHFR gene promoters in cancer and adjacent normal gastric mucosa specimens from 70 patients with gastric cancer, as well as normal gastric biopsy samples from 30 people without cancer serving as controls. In addition, them RNA expression of p16, Runx3, DAPK and CHFR was investigated in 34 gastric cancer patients by RT-PCR. Bisulfite DNA sequence analysis was applied to check the positive samples detected by MSP. Results. When carcinoma specimens were compared with adjacent normal gastric mucosa samples, a significant increase in promoter methylation of p16, Runx3, DAPK and CHFR was observed, while all 30 histologically normal gastric specimens were methylation free for all 4 genes. The methylation rate of the 4 genes increased from normal stomach tissue to tumor-adjacent gastric mucosa to gastric cancer tissue. Concurrent methylation in 2 or more genes was found in 22.9% of tumor-adjacent normal gastric mucosa and 75.7% of cancer tissues. No correlation was found between hypermethylation and other clinicopathological parameters such as sex, age, and tumor location. However, the frequency of DAPK and CHFR methylation in cancer tissues was significantly associated with the extent of differentiation and lymph node metastasis (P <0.05) and the frequency of Runx3 methylation was significantly associated with tumor size (P <0.05). Weak expression and loss of expression of the 4 genes was observed in cancer tissues and was significantly associated with promoter hypermethylation (P <0.05). Conclusions. Promoter hypermethylation of p16, Runx3, DAPK and CHFR is frequent in gastric cancer. DAPK and CHFR promoter hypermethylation may be an important help in evaluating the differentiation grade and lymph node status of gastric cancer. Weak gene expression and loss of gene expression due to promoter hypermethylation may be a cancer-specific event. Free full text available at www.tumorionline.it.

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