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Chen J.,Anhui Medical College | Sun T.,Shandong University | Chen C.-Y.,Shandong University
Chinese Journal of Cancer Prevention and Treatment | Year: 2016

OBJECTIVE: With the development of molecular biology and molecular oncology, the use of key molecules which is important in development of tumor as diagnostic markers and therapeutic targets has become a new trend. This research summarizes the advancements in findings about FoxM1, the transcription factor belonging to the Forkhead family. this article explores the role of FoxM1 in tumorigenesis, development, molecular diagnosis and targeted therapy. METHODS: A comprehensive search on PubMed and CNKI databases was performed with “FoxM1 and tumor” as key words to find all related manuscripts published from 2011-03 to 2016-02. A total of 383 articles in English and 97 articles in Chinese were collected. INCLUSION CRITERIA: Role of FoxM1 in tumorigenesis and tumor progression; Role of FoxM1 in diagnosis and targeted therapy of tumors. In total, 44 articles were selected according to the mentioned criteria. RESULTS: The investigated articles show that FoxM1, the transcriptional factor which belongs to Foxhead superfamily, can affect multiple biological processes, such as cell mitosis, cell cycle, DNA damage repair, cell proliferation and differentiation. This result is achieved by regulating the expression of Skp2, Cks1 and C-myc, all of which promote the transformation of G1/S and G2/M, and the cell cycle regulators which include Cdc25A, Cdc25B, Cyclin D1, p27kip1 and p21cip1. Literature also shows that overexpression of FoxM1 may lead to abnormal regulation of multiple aspects of cell proliferation, angiogenesis, epithelial-mesenchymal transition, maintaining characteristics of stem cells, metabolism and escape of senescence that promote cells malignant transformation and participate in the carcinogenesis and development. FoxM1 regulates drug sensitivity and resistance in a variety of tumor cells. Quantitative index diagnostic system based on expression of FoxM1 shows good accuracy in the early diagnosis of cancer. Anti-tumor compounds inhibit overexpressed FoxM1 and promote the process of FoxM1 as clinical therapeutic target. CONCLUSIONS: Overexpression of FoxM1 plays an important role in carcinoma and development. The detection of FoxM1 may be helpful on early identification of tumorigenesis. The specific inhibition of FoxM1 can be targeted intervention of tumorigenesis and development; FoxM1 is a potential target for tumor diagnosis and treatment. © 2016, Editorial Board of Chinese Journal of Cancer Prevention and Treatment. All right reserved.


Zhang Y.,Anhui University of Science and Technology | Zhang Y.,Anhui Medical College | Shi B.,Anhui University of Science and Technology
Journal of Digital Information Management | Year: 2017

At present, the traditional convolutional neural network (CNN) can easily cause overfitting in the training process, thereby resulting in an invalid training model. Thus, this study proposed a novel CNN regularization method to avoid overfitting in the training process and to increase the image classification accuracy of CNN. The proposed method uses failure probability as the theoretical basis. First, the failure probability density (FPD) function of image pixel points was deduced, and the FPD prediction model of pixel points in image neighborhood was established. Second, this method evaluated the FPD of image eigenvalues, selected the pixel points with the minimum FPD in the image neighborhood as the retention characteristics, and extracted excellent features. Finally, this method conducted a labeling classification experiment on three image classification datasets (MNIST, CIFAR-10, and CIFAR-100) and compared the image classification accuracy of the three existing popular pooling methods (Max, Average, and Stochastic pooling). Results demonstrated that the proposed regularization method based on FPD can increase the classification accuracy of the image data. The classification accuracies gained by the training datasets are all higher than 90%, and the classification accuracies gained by the testing datasets are all higher than 85%. The proposed CNN regularization method possesses good generalization capability and engineering applicability.


Song X.,Anhui Medical College | Wang Y.,Anhui Medical College | Cai J.,Anhui Medical College | Lu S.,China Institute of Technology | Chen Y.,China Institute of Technology
Journal of Radioanalytical and Nuclear Chemistry | Year: 2013

The interaction of U(VI) with Na-attapulgite was studied by using batch technique at different experimental conditions. The effect of contact time, solid content, pH, ionic strength and temperature on the sorption of U(VI) onto Na-attapulgite in the presence and absence of humic acid was also investigated. The results showed that the sorption of U(VI) on Na-attapulgite achieved sorption equilibrium quickly. Sorption of U(VI) on Na-attapulgite increased quickly with increasing pH at pH < 6.5, and then decreased with pH increasing at pH > 6.5. The sorption curves were shifted to left in low NaClO 4 solutions as compared those in high NaClO4 solutions. The sorption was strongly dependent on pH and ionic strength. The sorption was dominated by ion exchange or outer-sphere surface complexation at low pH values, and by inner-sphere surface complexation or surface precipitation at high pH values. The thermodynamic parameters (i.e., ΔH0, ΔS 0, and ΔG 0) for the sorption of U(VI) were calculated from the temperature dependent sorption isotherms, and the results suggested that the sorption reaction was an endothermic and spontaneous process. The Na-attapulgite is a suitable material in the removal and preconcentration of U(VI) from large volumes of aqueous solutions in nuclear waste management. © 2012 Akadémiai Kiadó, Budapest, Hungary.


Wang L.,Anhui University | He L.-Y.,Anhui Medical University | Meng D.-D.,Anhui Medical College | Chen Z.-W.,Anhui Medical University | And 5 more authors.
Parasites and Vectors | Year: 2015

Background: Recent studies have indicated the predominance of Toxoplasma gondii genotype Chinese 1 in animals in China. However, little is known of the genetic features of the parasite in humans. This study aims to determine the prevalence of anti-T. gondii antibodies based on which the genetic character of the parasite was identified in cancer patients in China. Methods: A total of 1014 serum samples with malignant neoplasms were collected from six tertiary-care hospitals (HAUCM, APH, HAMU, XAH, FHH and HBMC) from January, 2012 to August, 2013. Antibodies against T. gondii were examined by enzyme-linked immunosorbent assay (ELISA). Blood samples were subsequently used for PCR assay to detect T. gondii DNA (gra6). The DNA positive samples were subjected to genotyping using a multiplex multilocus nested PCR-RFLP at 10 loci, including sag1, sag2, sag3, btub, gra6, l358, c22-8, c29-2, pk1 and apico. Samples from the patients were anonymous and only data with regard to age and gender was available at sample collection. Results: Overall, 8.38% (85/1014) of the examined patients showed positive antibodies against T. gondii. Among them, 61 (6.02%) were seropositive only for IgG, 16 (1.58%) were only for IgM, and 8 (0.79%) were found to be positive for both IgG and IgM. The seroprevalence of antibodies to Toxoplasma ranged from 5.8% to 11.0%, without regional difference (χ 2∈=∈4.764, P∈=∈0.445). No significant differences of the positive rates of T. gondii infection were noted in genders (male, 8.96%; female, 7.45%) (χ 2∈=∈0.707, P∈=∈0.400) and in ages (χ 2∈=∈1.172, P∈=∈0.947). Of 1014 DNA samples, 36 (3.55%) were positive for T. gondii by nested PCR at gra6 locus and nine gave rise to complete genotyping results. All samples with achieved PCR-RFLP genotyping showed a common genetic character of type Chinese 1 (ToxoDB#9). Conclusion: Seroprevalence of toxoplasmosis in immunosuppressed individuals is rarely reported in China and we presented a positive rate of 8.38% in cancer patients. Toxoplasma genomic DNA genotyping demonstrated a common genetic character of Chinese 1, indicating a possible pathogenic origin of animals in human infection. © 2015 Wang et al.; licensee BioMed Central.


Bo Y.,Anhui Medical College | Yuan L.-P.,Anhui Medical University
Indian Journal of Pediatrics | Year: 2012

Objective To investigate the distribution and expression of apelin in kidney of adriamycin (ADR)-induced nephrotic rats, and to explore the possible association of apelin expression with the development of proteinuria. Methods Immunohistochemical staining for apelin was used to examine the expression and distribution of apelin in kidney of adriamycin (ADR)-induced nephrotic rats at 7, 14, 21 and 28 d after adriamycin injection. The level of serum apelin at 7, 14, 21 and 28 d after ADR injection was also determined with ELISA methods. Moreover the correlation of urine protein and glomerular expression of apelin was analyzed. Results The apelin protein was immunohistochemically detected along glomerular basement membrane (GBM) in the kidney of rats and significantly increased compared with normal control rats after ADR injection. The levels of 24 h proteinuria and serum apelin were also elevated after ADR injection. Moreover the production of proteinuria was correlated positively with the expression of apelin in kidney tissue (rs=0.9427, P<0.01). Conclusions The glomerular expression of apelin increased significantly in ADR rats, which is correlated with urine protein. © Dr. K C Chaudhuri Foundation 2011.


Wang M.,Anhui Medical University | Tao X.,Anhui Medical University | Tao X.,Bengbu Medical College | Song X.,Anhui Medical College
Journal of Radioanalytical and Nuclear Chemistry | Year: 2011

Oxidized multiwalled carbon nanotubes (MWCNTs) were characterized by SEM and FTIR. The sorption of Th(IV) on MWCNTs was studied as a function of contact time, pH, ionic strength, Th(IV) concentration and temperature. The results indicate that the sorption of Th(IV) on MWCNTs is strongly dependent on pH and weakly dependent on ionic strength. The sorption thermodynamics of Th(IV) on MWCNTs was carried out at 293.15, 313.15 and 333.15 K, respectively, and the thermodynamic parameters (standard free energy changes (ΔG 0), standard enthalpy change (ΔH 0) and standard entropy change (ΔS 0)) were calculated from the temperature dependent sorption isotherms. The sorption of Th(IV) on MWCNTs is a spontaneous and endothermic process. The oxidized MWCNTs may be a promising candidate for the preconcentration and solidification of Th(IV), or its analogue actinides from large volumes of aqueous solutions. © 2011 Akadémiai Kiadó.


Song X.,Anhui Medical College | Wang Y.,Anhui Medical College | Cai J.,Anhui Medical College
Journal of Radioanalytical and Nuclear Chemistry | Year: 2013

Bentonite has been studied extensively because of its strong sorption and complexation ability. In this study, GMZ bentonite (China) was studied as a potential sorbent for the removal of Th(IV) from aqueous solutions. The results indicate that the sorption of Th(IV) is strongly dependent on pH and ionic strength at pH <5, and is independent of ionic strength at pH >5. Outer-sphere surface complexation or ion exchange in inter-layer sites of the montmorillonite fraction of the GMZ bentonite may be the main sorption mechanism of Th(IV) onto GMZ bentonite at low pH values, whereas the sorption of Th(IV) at pH >5 is mainly dominated by inner-sphere surface complexation or surface precipitation. The presence of soil fulvic acid has a positive influence on the sorption of Th(IV) on GMZ bentonite at pH <5. The competition between Th(IV) with aqueous or surface adsorbed cation ions (e.g., herein Li+, Na+ and K+) and surface functional groups of GMZ bentonite is important for Th(IV) sorption on GMZ bentonite. The results of high sorption of Th(IV) suggest that the GMZ bentonite is a suitable backfill material in nuclear waste management. © 2012 Akadémiai Kiadó, Budapest, Hungary.


Dong L.,Anhui Medical College
Chinese Journal of Oncology | Year: 2011

Objective: To investigate the expression of p38 mitogen-activated protein kinase (p38MAPK) in salivary adenoid cystic carcinoma (SACC) tissues and their clinicopathologic significance. Methods: The protein and mRNA expressions of p38MAPK were examined by immunohistochemistry and in situ hybridization, respectively, in 52 cases of SACC and in 11 normal salivary gland tissues adjacent to the tumors on a tissue microarray platform. Results: The positive rate of p38MAPK protein expression in the paracancerous normal salivary gland tissues and that in SACC were 36.4% and 96.2%, respectively, showing a significant statistical difference (P < 0.01). The protein expression of p38MAPK was positively correlated with the lymph node metastasis and nerve involvement (P < 0.05). The positive rates of p38MAPK mRNA in the paracancerous tissues and in the SACC tissues were 45.5% and 94.2%, respectively, with a significant statistical difference (P < 0.01). The mRNA expression of p38MAPK was positively correlated with the lymph node metastasis and nerve involvement (P < 0.05). In the SACC, there was a notable positive correlation between the p38MAPK protein and mRNA expressions (r = 0.409, P < 0.01). Conclusions: The expression of p38MAPK is up-regulated in salivary adenoid cystic carcinoma. p38MARK may be involved in the tumorigenesis, development and metastasis of this cancer.


Wang X.,Anhui University | Zhang J.,Anhui University | Zou W.,Anhui University | Wang R.,Anhui Medical College
RSC Advances | Year: 2015

In this work, for the first time, novel polyaniline/carbon dot (PANI/Cdot) nanocomposites have been synthesized using a simple method. Meanwhile, the bright fluorescence of Cdots is effectively quenched by PANI through fluorescence resonance energy transfer. With the addition of Hg2+, the strong binding affinity between Hg2+ and the amino groups of PANI makes the Cdots break away and release from the PANI, resulting in the restoration of Cdot fluorescence, which can be used as a fluorescent probe for Hg2+ detection. This fluorescence "off-on" signal is sensitive to the concentration of Hg2+, and there is a good linear relationship between the fluorescence intensity of Cdots and Hg2+ concentrations in the range of 0.05 to 1.0 μM. The detection limit for Hg2+ at a given concentration is 0.8 nM. Moreover, it turns out that the nanoprobes represent a rather high selectivity for Hg2+ detection. We believe that PANI/Cdot composites will emerge as a new class of fluorescence materials that could be very likely to be suitable for practical applications. © The Royal Society of Chemistry 2015.


Objective: To assess the association of tumour necrosis factor-α (TNF-α) gene polymorphisms at positions -863C/A, -857C/T, -238G/A and Graves disease (GD) susceptibility in Chinese Han population in Anhui region. Methods: The polymorphisms of TNF-α gene were determined by polymerase chain reaction with specific primers in 254 patients affected with GD and 212 healthy controls. Allelic and genotypic frequencies in GD group and normal controls as well as in different genders were compared. The allelic and genotypic frequencies for different thyroid stimulating hormone receptor antibody (TRAb) levels (TRAb > 12 U/L; ≤12 U/L) were also compared among patients with earlier onset GD. Results: (1) The A allele at -863C/A locus in GD group (16.73%) was significantly greater than that of the control group (11.79%) (P<0.05, OR=1.503); the frequency of AA + CA genotype of -863C/A locus in GD group (32.68%) was significantly greater than that of control group (23.58%) (P<0.05, OR = 1.573). There was no significant difference (P>0.05) in the allelic and genotypic frequencies of -857C/ T, -238G/A loci between the two groups. (2) There was no significant difference (P>0.05) in the allelic and genotypic frequencies of -863C/A, -857C/T, -238G/A loci between patients of different genders. (3) There was no significant difference (P>0.05) in such frequencies between patients with earlier onset GD and different TRAb levels (TRAb >12 U/L, ≤12 U/L). Conclusion: (1) The -863 A allele of TNFα gene may contribute to the development of GD in Chinese Han population in Anhui, whilst -857C/T, -238G/A alleles may not. (2) There is no association between TNF-α gene -863C/A, -857C/T, -238G/ A polymorphisms and development of GD in different genders. (3) There was no association between above polymorphisms and TRAb levels in patients with earlier onset GD.

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