Anhui Key Laboratory of Crop Biology

Hefei, China

Anhui Key Laboratory of Crop Biology

Hefei, China
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Shah L.,Anhui Agricultural University | Shah L.,Key Laboratory of Wheat Biology and Genetic Improvement on South Yellow and Huai River Valley | Shah L.,National United Engineering Laboratory for Crop Stress Resistance Breeding | Si H.,Anhui Agricultural University | And 21 more authors.
Physiological and Molecular Plant Pathology | Year: 2017

The selection for Fusarium head blight (FHB) resistance is a bit difficult in breeding programs due to the complex inheritance of the resistance genes and the climatic effect on disease vigorous and expression. The present study was performed to assess microsatellite markers related to the resistance against FHB and examine the efficiency of these microsatellite markers while choosing for the resistance genes in all populations derived from a resistant parent. Both recombinant inbred lines (RIL) of Annong1124 (02P67/Aizao87//Yangmai158) and segregating lines of Zhoumai–27 × Shengxuani–6 were evaluated for their resistance to FHB by inoculating with Fusarium graminearum in the greenhouse as well as in the field. A total of 19 and 15 microsatellite markers from the chromosomes 3BS and 5A, respectively, were applied to RIL and each population of Zhoumai–27 (F2, F3). On the other hand, seven microsatellite markers from chromosomes 4B were applied to F2 and F3 segregating populations but there was a general lack of marker polymorphism on 4B for the employed parents of RIL. The most significant SSR markers were detected on the chromosome 3B and 5A explaining 7.30% and 21.74% of phenotypic variation, respectively, for the resistance in the RIL. The best quantitative trait loci (QTL) were detected on 4B for F2 and F3 populations with marker interval of WMC238-GWM66 with the LOD score 40.49 and 41.52, respectively. The phenotypic variations for F2 and F3 on chromosome 5A were detected with the account of 0.49 and 2.33 (BARC197-GWM179), respectively. The additive effects on chromosomes 3BS, 4B, and 5A were clearly higher in F3 population as compared to the F2 population. SSR markers on the chromosome 3BS in addition to SSR markers on 4B and 5A have the potential for improving the wheat cultivars with improved FHB resistance through the use of marker-assisted selection. © 2017 Elsevier Ltd


Shah L.,Anhui Agricultural University | Shah L.,Key Laboratory of Wheat Biology | Shah L.,National United Engineering Laboratory for Crop Stress Resistance Breeding | Ali A.,Anhui Agricultural University | And 15 more authors.
Czech Journal of Genetics and Plant Breeding | Year: 2017

Wheat, one of the world’s major crops, is seriously affected by fungal diseases, especially in regions with high moisture and moderately warm temperatures. This paper reviews various molecular and conventional techniques that are used to identify genotypes with resistance alleles associated with Fusarium head blight (FHB) diseases. Quantitative trait loci (QTL) type II, designated as Fhb1, are frequently applied in plant breeding, and the newly recognized genes related to resistance to this fungal disease give extra insights into marker-assisted selection (MAS). Molecular markers are robust tools that may be routinely used in MAS for the mapping of resistance genes in crop breeding. FHB resistance is polygenic, and different resistance genes could be conveyed into a single genotype by MAS, which might ensure greater resistance to FHB disease. In conclusion, different researchers have used various techniques to control FHB resistance, such as MAS, gene pyramiding (through backcross), and molecular markers (association with resistance QTLs or genes). © 2017, Czech Academy of Agricultural Sciences. All rights reserved.


Si H.,Anhui Agricultural University | Si H.,Key Laboratory of Wheat Biology and Genetic Breeding in Southern Huanghuai Wheat Region | Si H.,Anhui Key Laboratory of Crop Biology | Zhou Z.,Anhui Agricultural University | And 4 more authors.
Molecular Breeding | Year: 2012

Polyphenol oxidase (PPO) is a major cause of time-dependent darkening and discoloration in Asian noodles and other wheat-based products. One of the best ways to reduce this undesirable darkening is to breed new wheat cultivars with low PPO activity using efficient and reliable markers. Based on the sequence of a PPO gene SSPPO-B1 (GenBank accession no. AB254804) located on chromosome 2B of common wheat, 26 pairs of primers were designed to detect polymorphisms between wheat cultivars with low and high PPO activity. F-8, one of these primer pairs, amplified double fragments (band "a" of approximately 400 bp and band "b" of approximately 600 bp) in the cultivars with low PPO activity, and a single fragment (only band a) in the cultivars with high PPO activity. The differences between the fragments a and b include five indels and several single nucleotide polymorphisms, which occurred in intron II of the PPO gene. F-8 can be used as a sequence-tagged site marker to discriminate between two alleles Ppo-B1a (GQ303713) and Ppo-B1b (AB254804). The screening of 284 accessions of the core collection of Chinese wheat germplasms using the marker F-8 showed that the double fragments were present in 188 accessions, and the single fragments were present in the remaining 96 accessions. Statistical analysis revealed that the cultivars with the double fragments had significantly lower mean PPO activity than those with the single fragments. We also screened the 284 accessions using two additional markers, PPO18 for Ppo-A1 on chromosome 2A and STS01 for Ppo-D1 on chromosome 2D. Results showed that the combination of markers F-8, PPO18, and STS01 could reliably predict PPO activity. These markers can be used in wheat breeding programs for low PPO activity selection to improve the quality of wheat-based products. © 2012 Springer Science+Business Media B.V.


Liu F.,Anhui Agricultural University | Liu F.,Key Laboratory of Wheat Biology and Genetic Improvement on South Yellow and Huai River Valley | Si H.,Anhui Agricultural University | Si H.,Key Laboratory of Wheat Biology and Genetic Improvement on South Yellow and Huai River Valley | And 10 more authors.
Scientific Reports | Year: 2016

The allohexaploid bread wheat originally derived from three closely related species with A, B and D genome. Although numerous studies were performed to elucidate its origin and phylogeny, no consensus conclusion has reached. In this study, we cloned and sequenced the genes Wcor15-2A, Wcor15-2B and Wcor15-2D in 23 diploid, 10 tetraploid and 106 hexaploid wheat varieties and analyzed their molecular evolution to reveal the origin of the A, B and D genome in Triticum aestivum. Comparative analyses of sequences in diploid, tetraploid and hexaploid wheats suggest that T. urartu, Ae. speltoides and Ae. tauschii subsp. strangulata are most likely the donors of the Wcor15-2A, Wcor15-2B and Wcor15-2D locus in common wheat, respectively. The Wcor15 genes from subgenomes A and D were very conservative without insertion and deletion of bases during evolution of diploid, tetraploid and hexaploid. Non-coding region of Wcor15-2B gene from B genome might mutate during the first polyploidization from Ae. speltoides to tetraploid wheat, however, no change has occurred for this gene during the second allopolyploidization from tetraploid to hexaploid. Comparison of the Wcor15 gene shed light on understanding of the origin of the A, B and D genome of common wheat. © The Author(s) 2016.


Si H.,Anhui Agricultural University | Si H.,Key Laboratory of Wheat Biology and Genetic Breeding in Southern Huanghuai Wheat Region | Si H.,Anhui Key Laboratory of Crop Biology | Zhao M.,Key Laboratory of Wheat Biology and Genetic Breeding in Southern Huanghuai Wheat Region | And 4 more authors.
The Scientific World Journal | Year: 2013

Sodium dodecyl sulfate (SDS) sedimentation volume has long been used to characterize wheat flours and meals with the aim of predicting processing and end-product qualities. In order to survey the influence of low-molecular-weight glutenin subunits (LMW-GSs) at Glu-B3 locus on wheat SDS sedimentation volume, a total of 283 wheat (Triticum aestivum L.) varieties including landraces and improved and introduced cultivars were analyzed using 10 allele-specific PCR markers at the Glu-B3 locus. The highest allele frequency observed in the tested varieties was Glu-B3i with 21.9% in all varieties, 21.1% in landraces, 25.5% in improved cultivars, and 12% in introduced cultivars. Glu-B3 locus represented 8.6% of the variance in wheat SDS sedimentation volume, and Glu-B3b, Glu-B3g, and Glu-B3h significantly heightened the SDS sedimentation volume, but Glu-B3a, Glu-B3c, and Glu-B3j significantly lowered the SDS sedimentation volume. For the bread-making quality, the most desirable alleles Glu-B3b and Glu-B3g become more and more popular and the least desirable alleles Glu-B3a and Glu-B3c got less and less in modern improved cultivars, suggesting that wheat grain quality in China has been significantly improved through breeding effort. © 2013 Hongqi Si et al.

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