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Yao J.,Anhui Agricultural University | Zhang L.,Anhui Agricultural University | Chen X.,Anhui Entry Exit Inspection and Quarantine Bureau | Li Z.,Anhui Agricultural University
Biocontrol Science and Technology | Year: 2012

Field survey of the entomopathogenic fungus Beauveria bassiana in association with the red turpentine beetle, Dendroctonus valens, was undertaken in three pine plantations in Northern China. In total, 88 strains of B. bassiana sensu lato were isolated from the soil, bark, beetle frass, living adult and cadaver samples and soil was proved to be an important inoculum reservoir for fungal entomopathogens. Of these, 77 isolates were included for genetic diversity analysis by PCR for inter-simple sequence repeats (ISSR). Genetic diversity and population structure analysis of the isolates from three sites and five niches demonstrated high genetic diversity and heterogeneity between and/or within populations. Wright's statistics revealed a high gene flow rate (4.529) among the three populations, especially among the soil-derived isolate subpopulations. Low variation was mainly caused (94.8%) by variation among different substrates, suggesting the importance of microhabitat substrates on genetic diversity of B. bassiana. Phylogenetic variation was not associated with geographic distance. © 2012 Copyright Taylor and Francis Group, LLC.


Liu G.,Hefei University of Technology | Yu X.,Anhui Entry exit Inspection and Quarantine Bureau | Xue F.,Jiangsu Entry exit Inspection and Quarantine Bureau | Chen W.,Hefei University of Technology | And 5 more authors.
Microchimica Acta | Year: 2012

We report on a rapid method for the detection of Salmonella O8. It does not require an enrichment step but rather uses an aptamer as a probe that was selected by system evolution of ligands by exponential enrichment (SELEX) assay. Firstly, aptamer against Salmonella O8 was selected from a 78 bp random DNA library that was prepared in-vitro. The binding ability of the aptamers to target bacterium was examined by aptamer-linked immobilized sorbent assay. A high affinity aptamer was successfully selected from the initial random DNA pool, and its secondary structure was also investigated. Next, this high affinity aptamer B10 was used to recognize Salmonella O8 via fluorescence microscopy. The selected aptamer has a high specificity and high affinity against its target. We believe that the resulting fluorescence in-situ labeling assay is a potentially useful alternative in rapid screening and detection of foodborne pathogens. © 2012 Springer-Verlag.


Chen Y.,Anhui Academy of Agricultural Sciences | Yao J.,Anhui Entry Exit Inspection and Quarantine Bureau | Wang W.-X.,Anhui Academy of Agricultural Sciences | Gao T.-C.,Anhui Academy of Agricultural Sciences | And 2 more authors.
European Journal of Plant Pathology | Year: 2013

Sensitivity to epoxiconazole of 90 single-spore isolates of Magnaporthe oryzae was determined. The EC50 values for epoxiconazole in inhibiting mycelial growth of the 90 M. oryzae isolates were 0. 11-0. 86 μg/ml, with an average EC50 value of 0. 260 ± 0. 082 μg/ml. There was no correlation between sensitivity to epoxiconazole and sensitivity to carbendazim or iprobenfos. In protective and curative tests, epoxiconazole applied at 200 μg/ml provided over 70 % control efficacy, while tricyclazole exhibited better protective than curative activity. The results of four field trials performed in 2010 and 2011 at two sites showed that epoxiconazole at 112. 5 g a. i/ha provided over 75 % control efficacy, which was similar to tricyclazole with 300 g a. i./ha and better than carbendazim with 562. 5 g a. i./ha. © 2012 KNPV.


Lu R.,Hefei University of Technology | Sheng G.-P.,Hefei University of Technology | Hu Y.-Y.,Anhui Entry Exit Inspection and Quarantine Bureau | Zheng P.,Anhui Entry Exit Inspection and Quarantine Bureau | And 3 more authors.
Biomass and Bioenergy | Year: 2011

Bio-oils usually contain many types of compounds with various chemical properties. A bio-oil sample derived from rice husk through rapid pyrolysis was fractioned using solvent- or solid-extraction techniques based on their various properties. Ultraviolet-visible spectroscopy, three-dimensional excitation-emission matrix (EEM) fluorescence spectroscopy and Fourier transform infrared spectroscopy were used to characterize their various spectral properties for further understanding the characteristics of the bio-oil. Bio-oil mostly contains many aromatic ring components, acidic polar fractions, few weak- and non-polar components. The results all show that the main compounds and functional groups in the various bio-oil fractions were different and depended on the fractionation methods. The compositions of the bio-oil fractions were also analyzed with a gas chromatography/mass spectrometry (GC/MS) method. The consistency of the results obtained from the spectrometric methods with the GC/MS method indicates that the spectrometric methods have a good potential for rapid and effective characterization of bio-oils. © 2010 Elsevier Ltd.


Liang J.,Hefei University of Technology | Xu J.,Hefei University of Technology | Pan J.,Hefei University of Technology | Ge M.,Hefei University of Technology | Zong K.,Anhui Entry Exit Inspection and Quarantine Bureau
International Journal of Food Properties | Year: 2015

The objective of this work was to identify the main allergenic proteins and to assess the influence of high hydrostatic pressure on the potential allergenicity of high hydrostatic pressure pineapple juice. Fresh pineapple juice was treated with different pressures and the allergenic proteins were extracted and analyzed by sodium dodecyl sulfate polyacrylamide gel electropheresis, Western bloting and liquid chromatography-tandem mass spectrometry. The main allergenic proteins were detected as 24-kDa and 14-kDa bands by serum of pineapple-allergic patients. liquid chromatography-tandem mass spectrometry analysis of these bands showed that they actually consisted of a mixture of several isotypes of cysteine proteinases. Among these, bromelain (Ananas comosus) is a known allergen named Ana c 2 and it is highly homologous to other cysteine proteinases. Enzyme-linked immunosorbent assay assay of high hydrostatic pressure pineapple juice carried out at ambient temperature (18-22°C) showed that the higher the pressure, the higher the reduction in allergenicity, yielding a maximum reduction of about 20% under 500 MPa. However, in the case of 400 MPa and 50°C, the reduction rate of allergenicity was higher, up to 50% compared to that of the sample at 20°C without high hydrostatic pressure treatment. Taken together, the results suggested that high-pressure treatment could alter the potential allergenicity of pineapple juice allergens and identified Ana c 2 and a few other related cysteine proteinases as the main allergens. © Taylor and Francis Group, LLC.


Liu T.,Anhui University of Science and Technology | Li B.,Anhui University of Science and Technology | Hao Y.,Anhui University of Science and Technology | Han F.,Anhui Entry Exit Inspection and Quarantine Bureau | And 2 more authors.
Applied Catalysis B: Environmental | Year: 2015

Incorporating plasmonic Ag nanoparticles into mesoporous metal-oxide (MMO) semiconductors will achieve collective effect to greatly increase the photocatalysis. This work demonstrated a general two-step method to obtain diverse Ag/MMO composite photocatalysts with plasmon-enhanced photocatalytic activity. Several typical MMO (TiO2, ZnO, and CeO2) semiconductors were synthesized by integrating evaporation-induced self-assembly and in situ pyrolysis of metal precursors. Different amounts of Ag nanoparticles were then loaded in these MMO semiconductors through a facile photodeposition process. The Ag nanocrystals with sizes of 50-100nm were embedded in MMO semiconductors, endowing the Ag/MMO composites notable visible light absorption. The photocatalytic activities of the as-prepared diverse photocatalysts were studied systematically. The influencing factors including MMO species, mesoporous structure, and Ag loading amount on the photocatalytic activity were discussed in detail. The Ag/MMO composites exhibited much improved photocatalytic activity than their pure MMO semiconductors. The mesoporous TiO2 with the Ag-loading amount of 5wt.% exhibited the best photocatalytic performance for photodegrading both methylene blue and phenol under a simulated sunlight. The enhancement in photocatalysis is attributed to the synergistic effect of the mesoporous structures for efficient mass transfer as well as the Ag nanoparticles providing plasmonic enhanced light absorption. © 2014 Elsevier B.V.


Liu G.-Q.,Hefei University of Technology | Lian Y.-Q.,Hefei University of Technology | Gao C.,Hefei University of Technology | Yu X.-F.,Anhui Entry Exit Inspection and Quarantine Bureau | And 4 more authors.
Journal of Integrative Agriculture | Year: 2014

Aptamers are specific nucleic acid sequences that can bind to a wide range of nucleic acid and non-nucleic acid targets with high affinity and specificity. Nucleic acid aptamers are selected in vitro from single stranded DNA or RNA ligands containing random sequences of up to a few hundred nucleotides. Systematic evolution of ligands by exponential enrichment (SELEX) was used to select and PCR amplify DNA sequences (aptamers) capable of binding to and detecting Listeria monocytogenes, one of the major food-borne pathogens. A simplified affinity separation approach was employed, in which L. monocytogenes in exponential (log) phase of growth was used as the separation target. A fluorescently-labeled aptamer assay scheme was devised for detecting L. monocytogenes. This report described a novel approach to the detection of L. monocytogenes using DNA aptamers. Aptamers were developed by nine rounds of SELEX. A high affinity aptamer was successfully selected from the initial random DNA pool, and its secondary structure was also investigated. One of aptamers named e01 with the highest affinity was further tested in aptamer-peroxidase and aptamer-fluorescence staining protocols. This study has proved the principle that the whole-cell SELEX could be a promising technique to design aptamer-based molecular probes for dectection of pathogenic microorganisms without tedious isolation and purification of complex markers or targets. © 2014 Chinese Academy of Agricultural Sciences.


Zhang L.,Anhui Entry Exit Inspection and Quarantine Bureau | Zhang L.,Anhui Province Key Laboratory of Analysis | Han F.,Anhui Entry Exit Inspection and Quarantine Bureau | Han F.,Anhui Province Key Laboratory of Analysis | And 8 more authors.
Analytica Chimica Acta | Year: 2012

A methodology based on chemometrics and quantum chemistry was proposed to design and synthesize dummy molecularly imprinted polymers (DMIPs) capable of extracting chloroacetamide herbicides from food samples. Molecular modeling approach in conjunction with clustering analysis was used to predict the most suitable dummy template. Metolachlor deschloro was selected as the template to synthesize DMIPs, which were used as the solid phase extraction (SPE) materials. The selective adsorption of DMIPs was evaluated by high performance liquid chromatography (HPLC). The retention property of six chloroacetamide herbicides on DMIPs was also predicted using clustering analysis. The optimum loading, washing and eluting conditions for dummy molecularly imprinted solid phase extraction (DMISPE) were established to obtain high selectivity and sensitivity. Water, dichloromethane and methanol were chosen as loading, washing and elution solvent, respectively. Under optimized DMISPE conditions, recoveries of analytes were in the range of 83.4-106.7% with satisfactory precision (RSD% lower than 13%). Compared with other commercial SPE columns, DMISPE exhibited selective binding properties for chloroacetamide herbicides and the matrix effect was significantly decreased. © 2012 Elsevier B.V.


Liu G.-Q.,Hefei University of Technology | Zhang L.-L.,Hefei University of Technology | Zong K.,Anhui Entry exit Inspection and Quarantine Bureau | Wang A.-M.,Hefei University of Technology | Yu X.-F.,Anhui Entry exit Inspection and Quarantine Bureau
Food Science and Technology Research | Year: 2012

This study aims to evaluate the effects of spices essential oils (SEOs) on the microbial populations in chilled pork stored in PE film antimicrobial package. The microbiota from the pork under different storage conditions were studied by Polymerase Chain Reaction-Denaturing Gradient Gel Electrophoresis (PCR-DGGE) of the V3 variable region of the 16S rRNA, followed by sequencing of DGGE fragments. We found that SEOs resulted in an overall significant reduction of viable counts of Gram positive bacteria such as Carnobacteria, Enterococcus sp., Staphylococcus sp. and Brochotrix thermosphacta compared to the control. A 2.5 log cycles reduction of enterobacteria was also identified compared to the control during storage, while SEOs had little effect on Pseudomonas sp. the total volatile basic nitrogen (TVBN)and Thiobarbituric acid reactive substance(TBARS) were affected by the use of SEO packages, but color (L*, a*, b*), shear force and cooking loss were not affected. The bacteriostatic effect of clove EO was the best among all the SEOs tested, and the number of spoilage populations decreased with the use of the SEOs, but the species diversity of spoilage microbiota was not affected. © 2012 Food Sci. Technol. Res.


PubMed | Anhui Entry Exit Inspection and Quarantine Bureau
Type: | Journal: Analytica chimica acta | Year: 2012

A methodology based on chemometrics and quantum chemistry was proposed to design and synthesize dummy molecularly imprinted polymers (DMIPs) capable of extracting chloroacetamide herbicides from food samples. Molecular modeling approach in conjunction with clustering analysis was used to predict the most suitable dummy template. Metolachlor deschloro was selected as the template to synthesize DMIPs, which were used as the solid phase extraction (SPE) materials. The selective adsorption of DMIPs was evaluated by high performance liquid chromatography (HPLC). The retention property of six chloroacetamide herbicides on DMIPs was also predicted using clustering analysis. The optimum loading, washing and eluting conditions for dummy molecularly imprinted solid phase extraction (DMISPE) were established to obtain high selectivity and sensitivity. Water, dichloromethane and methanol were chosen as loading, washing and elution solvent, respectively. Under optimized DMISPE conditions, recoveries of analytes were in the range of 83.4-106.7% with satisfactory precision (RSD% lower than 13%). Compared with other commercial SPE columns, DMISPE exhibited selective binding properties for chloroacetamide herbicides and the matrix effect was significantly decreased.

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