Anhui Anke Biotechnology (Group)

www.ankebio.com
Hefei, China
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Lu C.-Y.,Anhui Anke Biotechnology (Group) | Li Z.-L.,Anhui Anke Biotechnology (Group) | Zhou L.-C.,Anhui Anke Biotechnology (Group) | Wang R.-H.,Anhui Anke Biotechnology (Group) | Song L.-H.,Anhui Anke Biotechnology (Group)
Chinese Journal of Biologicals | Year: 2014

Objective: To develop,verify and preliminarily apply a high performance liquid chromatography (HPLC) method for determination of histidine content in recombinant human interferon (rhIFN) α22b injection. Methods: Under designed condition,HPLC was verified for specificity,system suitability, intra- and inter-precisions and accuracy, and determined for linear range and minimum detection limit. The histidine contents in six batches of rhIFN α22b injection were determined by HPLC method. Results: The HPLC method showed high specificity, system suitability, intra- and inter-precisons and accuracy, of which the linear range was 6. 064 5 ∼ 775 μ,g/ml (R2 = 0. 999 8), and the minimum detection limit was 50 ng/ml. The histidine contents in six batches of rhIFN α22b injection were similar to those stated. Conclusion: The developed HPLC method was accurate and reliable, which might be used for determination of histidine content in rhIFN α22b injection.


PubMed | Anhui Anke Biotechnology (Group) and Anhui University of Science and Technology
Type: | Journal: The international journal of biochemistry & cell biology | Year: 2016

Autophagic lysosomal reformation, a key cellular process for maintaining lysosome homeostasis in elevated autophagy, so far has only been reported for cells under certain forms of starvation. For this reason, it is controversial that whether this phenomenon is starvation-specific and its importance in lysosomal regeneration at the late stage of autophagy is often challenged. Here we show that exogenous hydrogen peroxide (H2O2) induced lysosome depletion and recovery characteristic of autophagic lysosomal reformation, and we confirmed the occurrence of autophagic lysosomal reformation after H2O2 treatment by demonstrating Rab7 dissociation from autolysosomes, recruitment of Phosphatidylinositol 4-phosphate (PI4P) and clathrin to the surface of autolysosomes, and the existence of tubular pro-lysosome structures extending from autolysosomes. Similar to starvation, H2O2 caused an initial deactivation and a subsequent reactivation for mTOR, and mTOR reactivation was essential for ALR. Our results provided a first non-starvation example of autophagic lysosomal reformation and provide evidence for its importance for some autophagic processes other than that of starvation.


PubMed | Anhui University of Traditional Chinese Medicine, Anhui Medical University and Anhui Anke Biotechnology (Group)
Type: Journal Article | Journal: Neural regeneration research | Year: 2015

Glutamate application is an established method of inducing PC12 cell injury. PC12 cells were cultured with serum containing Tongqiaohuoxue decoction consisting of moschus, Carthamus tinctorius, Rhizoma chuanxiong, Semen pruni persicae, and Radix Paeoniae Rubra. After 24 hours of co-cultivation, glutamate (12.5 mM) was added to the culture medium. We found that serum containing Tongqiaohuoxue decoction prevented the increase in reactive oxygen species, and the decreases in superoxide dismutase and Na(+)-K(+)-ATPase activity, induced by glutamate. It also reduced the concentration of malondialdehyde, enhanced the mitochondrial transmembrane potential, inhibited the elevation of cellular calcium, and decreased phosphorylation of calmodulin-dependent protein kinase II. Thus, serum containing Tongqiaohuoxue decoction had protective effects on cell proliferation and membrane permeability in glutamate-injured PC12 cells.


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Anhui Anke Biotechnology (Group) | Entity website


Anhui Anke Biotechnology (Group) | Entity website

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