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Li L.M.,Anhui Agricultural University | Li L.M.,Anhui Animal Biological Engineering Technology Research Center | Diao H.,Anhui Xinhua University | Ding X.L.,Anhui Agricultural University | And 2 more authors.
Journal of Animal and Veterinary Advances | Year: 2011

This study aimed at investigating the effect of Met or Lys as sole Nitrogen (N) and Carbon (C) sources on Glutamic Oxaloacetic Transaminase (GOT), Glutamic Pyruvic Transaminase (GPT) and Amino Acid (AA) metabolism of rumen microorganisms in vitro. Three fistulated goats were used as a source of ruminal microorganisms. Microbial suspensions with or without Met or Lys as substrate were anaerobically incubated at 39°C for 16 h. Free AA and transaminases in the supernatants of the incubation were analyzed by HPLC and an automatic biochemistry analyzer, respectively. When Met was used as a unique source of N and C, the content of free Val and His were significantly (p<0.01 and p<0.05) reduced but Gly was increased (p<0.01). No significant differences in the other free AA were found as well as for GOT and GPT activities. When Lys was used as a sole source of N and C, the content of free His and activities of GOT and GPT were significantly lower (p<0.01) than that of controls however Tyr was significantly increased (p<0.05). The results indicated that Val and Cys are probably the most important AA of rumen microorganisms when Met serves as the only source of N andC. But when Lys serves as a sole source of N and C, His becomes the most important AA. At the same time, GPT plays a weaker role when Met serves as a sole source of N and C. © Medwell Journals, 2011. Source


Fazhi X.,Anhui Agricultural University | Huihui P.,Anhui Agricultural University | Yang L.,Anhui Agricultural University | Lumu L.,Anhui Agricultural University | And 3 more authors.
Protein and Peptide Letters | Year: 2014

Protein hydrolysates are widely applied as antioxidants in nutrition, but the potential antioxidant activities of small peptides remain unknown. Therefore, we investigated the antioxidant activities of small peptides isolated from solid-state fermented sesame meal via Sephadex G-15 chromotography. The scavenging capacities for 2, 2- diphenylpicrylhydrazyl (DPPH) and hydroxyl (•OH) radicals as well as the total reducing capacity were determined. The in vivo antioxidant activity was determined upon 30-d intragastric administration of the isolated small peptides (tripeptide, tetrapeptide, and hexapeptide) at different doses (0.1, 0.2, and 0.4 g/kg•d) in healthy Kunming mice. The results showed that the DPPH and •OH scavenging rates of the three peptides exceeded 80%. The total reducing activities of 4 mg/mL tetrapeptide or hexapeptide and 2 mg/mL tripeptide were comparable to that of 0.5 mg/mL glutathione. In mice fed sesame peptides, malondialdehyde levels in the serum and liver were lower than those in controls, whereas the activities of liver superoxide dismutase and glutathione peroxidase were significantly higher than those in controls (P<0.05). The antioxidant activity of tripeptide was significantly higher than those of tetrapeptide and hexapeptide (P<0.05). In conclusion, small peptides extracted from solid-state fermented sesame meal possess strong antioxidant activities that increase with decreasing peptide molecular weight. © 2014 Bentham Science Publishers. © 2014 Bentham Science Publishers. Source


Hu H.-W.,Anhui Agricultural University | Li L.-M.,Anhui Agricultural University | Li L.-M.,Anhui Animal Biological Engineering Technology Research Center | Qian K.,Anhui Animal Biological Engineering Technology Research Center | And 6 more authors.
Advanced Materials Research | Year: 2013

In the research, the duck farm cushion and organic waste of duck hatching plant and slaughter and processing plant were used as compost materials, single factor 4 levels 3 replications design was carried out. Effects of adding different doses(0%, 0.35%, 0.40%0.40%, 0.45%) of microorganism agent (consisted of thermophilic Bacillus and Serratia) on compost physicochemical indexes including temperature, moisture content, C/N, germination index, total N, total P, total K, available N, available P, available K, and the enzymic activities including urease, cellulase, dehydrogenase, catalase and protease. There were four treatments in this experiment, three was with adding different doses(0.35%, 0.40%, 0.40%, 0.45%)of microorganism agents, one was without adding microorganism agent(control). The results showed that, adding microorganism agent treatment had faster temperature rising, higher temperature and longer high temperature duration than control. At the end of composting, C/N of those with adding microorganism agent were significantly lower than control(P<0.05), germination index, total N, total P, total K, available N, available P, available K were significantly higher than control(P<0.05). Urease, dehydrogenase, Catalase and protease activities were significantly higher than control(P<0.05), however, those with adding microorganism agent were not significantly higher than control(P>0.05).Enzyme activity of experimental treatments and the physicochemical index shows a good correlation. In conclusion, adding 0.35% microorganism agent was optimum for composting in this research. © (2013) Trans Tech Publications, Switzerland. Source


Xu F.,Anhui Agricultural University | Li L.,Anhui Agricultural University | Xu J.,Anhui Agricultural University | Qian K.,Anhui Animal Biological Engineering Technology Research Center | And 2 more authors.
Asian-Australasian Journal of Animal Sciences | Year: 2011

A trial was performed to study the effects of feeding a diet containing solid-state fermentation rapeseed meal (FRSM) replaced soybean meal (SBM) on growth performance and serum biochemistry parameters of ducks and then to determine the appropriate proportion of soybean meal replacement. The 75% rapeseed meal and 25% blood meal were mixed and inoculated with the Lactobacillus plantarum and Bacillus subtilis. Over the 21-day fermentation, isothiocyanates were reduced from 72.7 to 14.1 mmol/kg. A total of 1,280 fifteen-day-old Cherry Valley ducks were randomly allocated into 4 dietary treatments, 4 replicate groups of 80 ducks each for a 30-day feeding trial. In four treatment groups, fermentation rapeseed meal replaced soybean meal at 0, 33, 67 or 100%, respectively. Results showed that feed intake of ducks fed 100% FRSM was greater (p<0.05) than SBM and partial FRSM in both the finishing period (31-45 d) and entire feeding period (15-45 d). Daily gain increased gradually in the three treatment groups with augmenting FRSM over in the whole study period. In the growing period (15-30 d), compared with the SBM group, phosphorus and calcium content in serum from the FRSM group was improved (p<0.05). Total protein concentration was lower in ducks fed 100% FRSM than SBM and 33% FRSM (p<0.05). Concentrations of IgM were dramatically higher for animals fed 100% FRSM than in the SBM, 33% FRSM and 67% FRSM groups. In the finishing trail stage (31-45 d), only serum IgG content in 100% FRSM group was improved (p<0.05). Therefore, rapeseed meal fermented with Lactobacillus plantarum and Bacillus subtilis is a promising alternative protein source and fermented rapeseed meal can completely replace soybean meal in duck diet and potentially reduce the cost of duck production. Source


Li L.M.,Anhui Agricultural University | Li L.M.,Anhui Animal Biological Engineering Technology Research Center | Ding X.L.,Anhui Agricultural University | Ding X.L.,Anhui Animal Biological Engineering Technology Research Center | And 3 more authors.
Journal of Animal and Veterinary Advances | Year: 2011

In this study, we investigated the effect of microbes on the composting of pig manure. Geotrichum candidum, Yarrowia lipolytica, Bacillus coagulans, Bacillus stearothermophilus and Nocardia sp. were isolated from fresh pig feces. Fifteen different combinations of these microorganisms were added to a mixture of pig manure and sawdust for composting. An orthogonal, fractional factorial experimental design consisting of five factors and two levels was used. Statistical analysis indicated that Geotrichum candidum plus Bacillus stearothermophilus was, the most effective combination. The results indicated that the addition of microbes to feces raised the maximum temperature of composting from 67-72 oC and elevated the available nutrients by 9.5%. © 2011 Medwell Journals. Source

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