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Cao H.,Nantong University | Chen L.,Anhui Academy of Science and Technology | Xiao J.,Anhui Academy of Science and Technology | Xiao J.,Shanghai Normal University
Molecular Biology Reports | Year: 2010

Much of the bioactivities of Citrus flavanones significantly appear to impact blood and microvascular endothelial cells. It is essential to investigate the interaction between Citrus flavanones and serum albumin to verify the effect of flavanone structures on the distribution and transportation in blood. The interactions between flavonoids and proteins have attracted great interest among researchers. The work in here mainly concerns about the binding interaction between Citrus flavanones and human serum albumin (HSA) in vitro. The methoxylation of tangeretin improved the affinity for HSA by 100 times. The 2,3-double bond in conjugation with a 4-oxo group plays an important role for the affinity for HSA. The affinity of apigenin for HSA is about 10,000-times higher than that of naringenin. It was found that the hydroxylation on position 3′ of flavonol significantly improves the binding affinity for HSA. The affinity of quercetin (3′, 4′) for HSA is about 100-times higher than that of kaempferol (4′). The hydroxylation on position 3′ of flavone slightly improves the binding affinity for HSA. The affinity of luteolin for HSA is about 1.38-times higher than that of apigenin. The values of log10(Ka) are proportional to the number of binding sites (n), which confirms the method used here is suitable to study the interaction between Citrus flavanones and HSA. © 2010 Springer Science+Business Media B.V. Source


Chen T.,Anhui Academy of Science and Technology | Chen T.,Anhui University of Architecture | Zhang F.-J.,Anhui University of Architecture | Wu Z.-S.,Anhui University of Architecture | And 2 more authors.
Asian Journal of Chemistry | Year: 2012

Three kinds of iron oxide/graphene composites were prepared using three different ferric salts [FeCl 3, Fe(NO 3) 3, FeSO 4] to react with graphene oxide and followed by a heat treatment. Then, the structures of different iron oxide/graphene composites were compared and analyzed by IR spectra and scanning electron microscopy. After that the degradability using different iron oxide/graphene composites to degrade methylene blue was explored by ultraviolet spectra. The results showed that iron/graphene (FeCl 3 as iron source) composite has better degradability under sunlight than that of other iron/graphene composites (Fe(NO 3) 3 or FeSO 4 as iron source). Source


Zhang F.-J.,Anhui University of Architecture | Xuan H.,Anhui University of Architecture | Xie F.-Z.,Anhui University of Architecture | Jiang T.,Anhui University of Architecture | And 3 more authors.
Asian Journal of Chemistry | Year: 2012

Nanoscale zero valent iron-graphene has been synthesized via a simple effective chemical method. The composite nanosheets are super paramagnetic at room temperature and can be separated by an external magnetic field. The prepared nanoscale zero valent iron-graphene nanosheets were characterized by X-ray diffraction, transmission electron microscopy. The results demonstrated the successful attachment of nanoscale zero valent iron nanoparticles to graphene nanosheets. It was found that the nanoscale zero valent iron-graphene nanosheets not only show near complete methyl orange decolorization within 0.5 h under natural lighting, but they are also practically usable for methyl orange separation from water. Source


Zhang F.,Anhui University of Architecture | Xie F.,Anhui University of Architecture | Zhang K.,Pohang University of Science and Technology | Chen T.,Anhui Academy of Science and Technology | Oh W.,Hanseo University
Synthesis and Reactivity in Inorganic, Metal-Organic and Nano-Metal Chemistry | Year: 2012

Pt and Pd co-doped TiO 2 nanoparticles were synthesized using modified sol-gel processes and characterized by BET surface area, SEM, TEM, XRD, EDX, FT-IR, and UV-Vis spectroscopy. The results showed that the Pt/Pd-TiO 2 nanoparticles possess high surface area and small particles size compared with individual Pt or Pd doped TiO 2. The significantly high photocatalytic activity can be ascribed to the simultaneous effects of doped Pt and Pd by cascadal electron transfer from TiO 2 to Pd to Pt and reach surface excitation state. And the optimum molar ratio of Pt/Pd at 1.5 can effectively reduce the electron-hole recombination rate. © 2012 Copyright Taylor and Francis Group, LLC. Source


Liu Y.J.,Anhui Academy of Science and Technology | Tang Q.,China Institute of Technology | Fang L.,China Institute of Technology
Plant Disease | Year: 2016

Jiuhua Mountain fuzz tip, a cultivar of Camellia sinensis, is cultivated for tea leaves on more than 1400 ha in Qingyang County, Anhui Province, China. In a survey conducted from 2013 to 2014 in Qingyang County, a leaf blight of unknown etiology was observed on leaves of all ages and was severe in young leaves of the cultivar. In 2014, nearly 25% of the plants of Jiuhua Mountain fuzz tip in the orchards visited, which comprised approximately 200 ha, were affected. Initial leaf symptoms of this disease included brown, circular spots, each surrounded by a yellow to rust-brown margin, that gradually increased from 5 to 10 mm in diameter and developed into elliptical lesions. Lesions enlarged and coalesced, resulting in severely blighted leaves. In September 2014, symptomatic leaves were collected from 21 different fields, surface-sterilized in 1.5% sodium hypochlorite for 2 min, plated on potato dextrose agar (PDA), and incubated at 25°C for 7 days. Fifteen morphologically similar fungal isolates recovered produced colonies that were initially white and became light to dark gray. Conidiophores were monoblastic, single, and 7.2 to 9.6 μm in diameter. Single-celled, black, spherical to subspherical conidia (12.3 to 14.6 × 15.2 to 16.8 μm) were each borne on a hyaline vesicle at the tip of a conidiophore. On the basis of these morphological features, the isolates appeared to be Nigrospora sphaerica (Sacc.) Mason (Ellis 1971; Mason 1927). Isolate QY-6 was selected as a representative for molecular identification. The universal internal transcribed spacer (ITS) region of rDNA primer pair ITS4/ITS5 was used to amplify a DNA fragment of approximately 583 bp. The amplified PCR products were sequenced and, based on BLASTn analysis, were 99% similar to two N. sphaerica isolates (GenBank Accession Nos. FJ478134.1 and HQ608030.1). The sequence of isolate QY-6 was deposited in GenBank (KP731976). Pathogenicity tests were conducted using the isolate QY-6 on five leaves of three 12-month-old Jiuhua Mountain fuzz tip plants, asymptomatic tea leaves that were disinfested with 70% ethanol for 1 min. Leaves were pushpin-wounded, and placed in a plastic box on wet, sterilized filter paper. A 6-mm-diameter agar plug from the advancing margin of a 7-day-old culture was placed on the wound site. The plastic box was maintained at 25°C in a greenhouse with constant relative humidity of 80 to 85%, and a 12-h photoperiod/day. An equal number of control leaves were wounded and inoculated with noncolonized PDA agar plugs. Inoculated leaves exhibited small (2 × 5 mm2), brown-to-black lesions after five days, and expanded to an average of 18 × 22 mm2 15 days after inoculation. All of the inoculated leaves developed blight symptoms similar to those observed on naturally infected leaves in the field, while control leaves remained symptomless. The experiments were conducted three times and the fungus was recovered from the leaf symptoms and identified as N. sphaerica by morphological characteristics, confirming Koch’s postulates. Leaf blight of tea caused by N. sphaerica was reported in India (Dutta et al. 2015), but to our knowledge, this is the first report of N. sphaerica as a leaf pathogen on C. sinensis in China. © The American Phytopathological Society. Source

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