Anhui Academy of Forestry

Hefei, China

Anhui Academy of Forestry

Hefei, China
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Hu J.,Anhui Agricultural University | Shah F.A.,Anhui Agricultural University | Wu W.,Anhui Academy of Agricultural Sciences | Cao Z.,Anhui Academy of Forestry | And 2 more authors.
Pakistan Journal of Botany | Year: 2017

The aim of this study is to investigate and understand the dynamic changes and the possible functions of hormones, water and sugar content during the development of C. oleifera. We analyzed and compared WWC, WSS and five endogenous hormones (DHZR, ZR, GA, IAA and ABA) contents between May and August in C. oleifera plants at different ages from the same orchard. The results showed that WWC, WSS, DHZR, ZR, GA, IAA and ABA contents varied from 61.64%-74.15%, 21.24mg/g-62.44mg/g, 6.237ng/g-38.342ng/g, 6.215ng/g-16.721ng/g, 5.668ng/g-18.024ng/g, 24.753ng/g-147.507ng/g and 66.541ng/g-156.212ng/g, respectively. WWC showed a decrease-increase-decrease variation pattern, while the WSS contents demonstrated a generally increasing trend with increasing tree age. The levels of ABA in leaves sampled in August was higher than that in May, but the opposite was found for the GA and IAA levels. Tree ages were negatively correlated with ZR and GA concentration at the 1% or 5% significance level. The results of this study broaden our understanding of the interrelationships between phytohormones, WWC, and WSS content in the growth and development of C. oleifera. © 2017, Pakistan Botanical Society. All rights reserved.


Wei Y.,Chinese Academy of Forestry | Wei Y.,Zhejiang Provincial Key Laboratory of Tree Breeding | Liu Q.,Chinese Academy of Forestry | Dong H.,Chinese Academy of Forestry | And 4 more authors.
PLoS ONE | Year: 2016

Pinus massoniaia Lamb has gained more and more attention as the most important tree species for timber and forestation in South China. Gene expression studies are of great importance to identify new and elite cultivars. Real-time quantitative PCR, a highly sensitive and specific method, is commonly used in the analysis of gene expression. The appropriate reference genes must be employed to normalize the calculation program for ascertaining repeatable and significant results. Herein, eleven housekeeping genes were evaluated during different stages of P. massoniana post nematode inoculation in this study. Three statistical approaches such as geNorm, NormFinder and BestKeeper were selected to analyze the stability of candidate genes. The results indicated that U2af and â-TUB were the most stable reference genes. These two genes could be used for the normalization in most of the experiments of P. massoniana, while Histone and AK were the least stable ones. In addition, EF expressed at the lowest average Ct value was the most abundant candidate gene. As an important gene associated with defense mechanisms, ABC transporter was analyzed by qRT-PCR, and the results were used to confirm the reliability of two genes. The selected reference genes in the present study will be conducive to future gene expression normalized by qRT-PCR in P. massoniana. © 2016 Wei et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


Hu J.J.,Anhui Agricultural University | Wu W.,Anhui Academy of Agricultural Sciences | Cao Z.H.,Anhui Academy of Forestry | Wen J.,Anhui Agricultural University | And 2 more authors.
Pakistan Journal of Botany | Year: 2016

Camellia oleifera Abel originates from China and is high healthy effect food oil species. It is also a high additional plant in southern China and can help to keep some people of mountain area out of poverty. In recent years, climate change has been abnormal frequently. Abnormal low temperature in winter and late spring coldness may cause the hard hit to C. oleifera farmers. Freezing injury can be caused by sudden decreases in temperature in winter. However, C. oleifera varieties differ in their hardiness to low temperatures. The paper investigated cold-resistance mechanisms by determining and analyzing the morphological, physiological and biochemical characteristics of C. oleifera from eastern, western and southern Anhui, respectively. Sensitivity to low temperature was assessed via the number of leaves in spring shoots, leaf thickness, the activities of protective enzymes such as CAT, POD and SOD, and the inclusion contents of WSS, FPro, MDA, benzene-alcohol extracts and lignin. The results showed that C. oleifera varieties had different physiological and biochemical, and morphological responses to low winter temperatures. In different regions, the number of leaves, leaf thickness, WSS content, FPro content and MDA content varied from 5.2-7.8, 398.79μm-465.27μm, 23.41mg/g-24.74mg/g, 41.86μg/g-44.18μg/g and 10.08μmol/g-14.51μmol/g, respectively. The varieties from eastern Anhui, the leaf thickness were thicker. Meanwhile, the protective enzyme activities and inclusion contents were relatively higher. The protective enzyme activities and chemical components contents such as benzene-alcohol extract and lignin represented significantly difference (p<0.05) among three regions. In the future, for the abnormal low temperature in winter, a serious of cultivation measures such as improving the contents of WSS, FPro, benzene-alcohol extract and lignin, were taken to enhance the cold resistance of C. oleifera. The result broadens the understanding of cold-resistance mechanisms in C. oleifera. © 2016, Pakistan Botanical Society. All Rights reserved.


Wu W.,Anhui Agricultural University | Wu W.,Anhui Academy of Agricultural Sciences | Hu J.,Anhui Agricultural University | Duan W.,Chinese Academy of Forestry | And 3 more authors.
Pakistan Journal of Botany | Year: 2015

The correlation between chemical components of shoot and leaf of Camellia oleifera and elevations were discussed, to provide theoretical basis for taking effective cultivation measures to promote the cold resistance of C. oleifera in the future “Northward Movement of C. oleifera”. The ratio of bound water to free water of the leaf, as well as the contents of holocellulose, cellulose, lignin and benzene-ethanol extracts were determined. The results showed that: In different elevations, the ratio of bound water to free water of the leaf varied from 0.11-0.22, the contents of holocellulose, cellulose, lignin and benzene-ethanol extracts varied from 25.08%-62.72%, 7.72%-36.61%, 11.80%-30.29% and 7.66%-8.57% respectively. In the same elevation, the content of benzene-ethanol extracts of leaf was higher than that of shoot, and the contents of other materials were quite the contrary. The elevation was positively correlated with the ratio of bound water to free water and the contents of holocellulose, cellulose, lignin and benzene-ethanol extracs. The chemical components of shoot and leaf represented extremely significant difference (p<0.01) among different elevations. In the future northward movement of C. oleifera, for the climate characteristics of cooler regions and higher elevation, a serious of cultivation measures, improving the contents of holocellulose, cellulose, lignin, benzene-ethanol extracts and the ratio of water to free water, were taken to improve the cold resistance of C. oleifera. © 2015, Pakistan Botanical Society. All rights reserved.


Zhu H.,Anhui Agricultural University | Wang Z.X.,Anhui Agricultural University | Luo X.M.,Anhui Academy of Forestry | Song J.X.,Anhui Agricultural University | Huang B.,Anhui Agricultural University
Journal of Agricultural Science | Year: 2014

SUMMARY Incorporation of rice straw into soil has traditionally been an important method of recycling nutrients and improving soil productivity. Currently, although the effects of straw incorporation on disease severity have been documented, the dynamics of the pathogen in soil after straw incorporation are poorly understood. In the present study, rice straw with various proportions of diseased straw was incorporated at three separate locations (SuPu town, SuSong County and FengYang County) in Anhui province, China. The pathogen dynamics in paddy soil and disease severity of sheath blight during two continuous years from April 2010 to April 2012 were investigated. For all three locations, the amount of pathogen inoculum that persisted in the soil increased with increases in the proportion of diseased straw incorporated. Incorporation of 0·3 and 0·5 diseased straw into soil increased the amount of pathogen inoculum in the soil significantly, whereas incorporation of 0·1 diseased straw into soil had no significant effect on the pathogen inoculum compared with the control (no straw incorporated) or disease severity. Incorporation of healthy rice straw (no disease) resulted in a significant decrease in disease severity, whereas proportions of 0·3 and 0·5 diseased straw resulted in a significant increase of disease severity compared with the control. These results suggested that incorporation of diseased straw enhanced pathogen numbers in soil during the whole decomposition period and increased disease severity. To avoid soil-borne disease accumulation, severely diseased straw should be removed from the field or pre-treated before incorporation. © Cambridge University Press 2013.


Zhu H.,Anhui Agricultural University | Luo X.-M.,Anhui Academy of Forestry | Song J.-X.,Anhui Agricultural University | Fan M.-Z.,Anhui Agricultural University | Li Z.-Z.,Anhui Agricultural University
Chinese Journal of Applied Ecology | Year: 2011

A Beauveria bassiana strain Bb2l was isolated from naturally infected green peach aphid Myzus persicae (Hemiptera: Aphididae). The effects of the strain on M. persicae and its two predaceous natural enemies Chrysoperla carnea (Neuroptera: Chrysopidae) and Harmonia axyridis (Co-leoptera; Coccinellidae) were investigated under laboratory conditions. Bb21 had strong pathoge-nicity to M. persicae, with the LD 50 of 97 conidia · mm -2 (45-191, 95% confidence interval), but was less pathogenic to the second instar nymph of C. carnea, with the LD 50 of 1089 conidia · mm -2. The LD 50 for C. carnea was 10.2 times higher than that for M. persicae. The pathogenicity of Bb21 to H. axyridis was very weak, with a low infection rate of 13% even at a high concentration 5×10 8 conidia · mL -1. The Bb21 at low conidia concentration had less effect on the developmental period and fecundity of the two predaceous natural enemies. However, when applied at the high concentration 5×10 8 spores · mL -1, Bb21 shortened the larval stage of H axyridis averagely by 1.4 d and decreased the adult emergence rate and fecundity by 33% and 14%, respectively, and shortened the larval stage of C. carnea averagely by 0.7 d and decreased the adult emergence rate and fecundity by 24% and 11%, respectively. Since the LD 50 for green peach aphid was much lower than that for the two predaceous natural enemies, and had very low effect on the adult emergence rate and fecundity of the two predators at the concentration recommended for field spray, Bb21 could be applied as a biocontrol agent of M. persicae in the integrated management of pernicious organisms.


Hu Y.,Anhui Academy of Forestry | Zhang J.,Huangshan Institute of New Rural Construction | Jin X.,CAS Institute of Botany
Nordic Journal of Botany | Year: 2015

Bulbophyllum huangshanense, a new species of orchid from Anhui, China, is described and illustrated. This new species is close to B. taeniophyllum with pseudo-bulbs closely spaced along the rhizome, leathery leaf, entire dorsal sepal, toothed petals, the lateral edges of lateral sepals connate and fleshy lip, but differs from the latter in having roots arising from the node with the pseudo-bulb, inflorescence much shorter than leaf, flowers pure yellow, lip mobile, and stelidia broadly triangular. © 2015 The Authors.


Xu Q.,Chinese Academy of Forestry | Li Y.,Chinese Academy of Forestry | Pan Y.-F.,Zhejiang University of Science and Technology | Shao J.-Z.,Anhui Normal University | And 2 more authors.
Forest Research | Year: 2010

New recorded geographical distribution of three wedand herbaceous plants were found in Anhui Province. These species are Elatine triandra Schkuhr. of Elatinaceae, Carex capricornis Meinsh. of Cyperaceae, and Thesium refractum Mey. of Santalaceae. The specimens are preserved in the Herbarium of Chinese Academy of Forestry (CAF).


Xia S.,Anhui Academy of Forestry | Zhang Y.,Anhui Academy of Forestry | Zhang Y.,Anhui University of Architecture | Wang W.,Hubei University
Journal of Pure and Applied Microbiology | Year: 2014

In this study, we analyzed allelopathic effects of Pistacia chinesis Bunge on Oncomelania hupensis through triterpene sapogenin, a potential molluscicide. The snails were exposed to six different concentrations (0, 2, 5, 10, 20, and 40 mg/L) of triterpene sapogenin and under five periods (24 h, 48 h, 72 h, 96 h, and 120 h). The mortality of snails was positively correlated with the concentration of triterpene sapogenin and exposure time. The liver and intestine observation through transmission electron microscope displayed that 10 mg/L triterpene sapogenin caused apparent damages to the structure of O. hupensis liver. The esterase (EST) isozyme activity of O. hupensis treated by 10 mg/L triterpene sapogenins was higher than that of control between 24-48 h, then decreased after 72 h. It implicates that the extracted triterpene sapogenin from Pistacia chinesis Bunge is promising for controlling the snail. Meanwhile, it provides the foundation for constructing plant community of Pistacia chinesis to control O. hupensis.


PubMed | Chinese Academy of Forestry and Anhui Academy of Forestry
Type: Journal Article | Journal: PloS one | Year: 2016

Pinus massoniaia Lamb has gained more and more attention as the most important tree species for timber and forestation in South China. Gene expression studies are of great importance to identify new and elite cultivars. Real-time quantitative PCR, a highly sensitive and specific method, is commonly used in the analysis of gene expression. The appropriate reference genes must be employed to normalize the calculation program for ascertaining repeatable and significant results. Herein, eleven housekeeping genes were evaluated during different stages of P. massoniana post nematode inoculation in this study. Three statistical approaches such as geNorm, NormFinder and BestKeeper were selected to analyze the stability of candidate genes. The results indicated that U2af and -TUB were the most stable reference genes. These two genes could be used for the normalization in most of the experiments of P. massoniana, while Histone and AK were the least stable ones. In addition, EF expressed at the lowest average Ct value was the most abundant candidate gene. As an important gene associated with defense mechanisms, ABC transporter was analyzed by qRT-PCR, and the results were used to confirm the reliability of two genes. The selected reference genes in the present study will be conducive to future gene expression normalized by qRT-PCR in P. massoniana.

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