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Giuri S.,Dr. Giuri Stela Private Medical Office | Raica M.,Angiogenesis Research Center Timisoara | Munteanu M.,Victor Babes University of Medicine and Pharmacy Timisoara
Romanian Journal of Morphology and Embryology | Year: 2013

Purpose: To present a rare case of conjunctival myxoid liposarcoma, subtype round cells, that had a seven years follow up. Clinical observation: A 61-year-old female patient presents with a palpable, non-painful tumor, on the superior temporal bulbar conjunctiva of the right eye. The initial examination detects a fleshy tumor, orange in color, under the superior temporal bulbar conjunctiva, as well as two oval-shaped hyperpigmented conjunctival lesions, near the limbus at 10 o'clock, causing moderate blepharoptosis. Vision was normal, there was no diplopia, proptosis, afferent pupillary defect or lymphadenopathy; there was no orbital involvement in MRI. An isolated 15/15 mm tumor, with no connections with the eye socket, was excised. Histopathology revealed a poorly differentiated myxoid liposarcoma. Five recurrences occurred, of which four were treated by local excision and the last required exenteration. Repeat histopathology detects lipoblasts, small round cells, with immunohistochemistry positive for CD34, S100 and vimentin. The last two rapidly evolving and large recurrences, as well as pulmonary metastasis and finally death of the patient, underlined the aggressive character of round cell conjunctival liposarcoma. Conclusions: Conjunctival myxoid liposarcoma is characterized by numerous local recurrences, but the speed of the succession and volume of the recurrences may suggest a change in the underlying histopathological aspect, that is definitory for the therapeutical and prognostic approach of the case.

Ceausu R.A.,Angiogenesis Research Center Timisoara | Cimpean A.M.,Angiogenesis Research Center Timisoara | Nicodin A.,Victor Babes University of Medicine and Pharmacy Timisoara | Raica M.,Angiogenesis Research Center Timisoara
Romanian Journal of Morphology and Embryology | Year: 2013

Idiopathic pulmonary fibrosis is a severe disease, with unpredictable evolution that frequently leads to respiratory failure and death, despite some progresses made in the field of therapy. Basically, the bad prognosis and failure of therapy are the consequence of the lack of data about the molecular events that have as result the extensive fibrosis. Although the basic lesions were defined many years ago, the pathological classification of pulmonary fibrosis is controversial. In the present work, we analyzed the prognostic impact of basic microscopic lesions on a possible new classification that could be related to the patient outcome. For this purpose, we have investigated 20 cases with idiopathic pulmonary fibrosis and samples of lung parenchyma were obtained by video assisted thoracoscopy. The specimens were processed by usual histological technique and sections were stained with Hematoxylin-Eosin, Masson's trichrome and Gordon-Sweet silver staining. There were evaluated the lung architecture, the chronic inflammatory infiltrate, macrophages and fibrosis. The distribution and severity of each parameter was converted into points and finally graded from I to IV, with corresponding score from 1 to 12. We found four cases with degree II, 12 with degree III, and four with degree IV. Our results support the hypothesis that the evaluation of basic lesions could be the basis for a more objective classification and staging of lung fibrosis and, possibly, a better prognostic method and, eventually, a predictor for the response to targeted therapy.

Raica M.,Angiogenesis Research Center Timisoara | Raica M.,Victor Babes University of Medicine and Pharmacy Timisoara | Cimpean A.M.,Angiogenesis Research Center Timisoara | Cimpean A.M.,Victor Babes University of Medicine and Pharmacy Timisoara | And 6 more authors.
In Vivo | Year: 2015

The presence and distribution of lymphatic vessels and mast cells in the gingiva under normal and pathological conditions have been reported by several studies, but the relationship between them during inflammatory lymphangiogenesis is virtually unknown. The aim of the present study was to investigate the lymphatic microvessel density (LMVD) and mast cell density (MCD) in the gingiva of patients with periodontal disease compared to normal-like gingiva. Gingival punch biopsies from 51 patients with periodontal disease were investigated. MCs and LVs were detected by double-immunohistochemistry, using primary antibodies against mast cell tryptase and D2-40. The inflammatory infiltrate was evaluated on a scale from 0 (absent) to +3 (severe inflammation). MCs and LVs were counted in the same microscopic field for each case at ×200 magnification. We found a significant increase in the number of both MCs and LVs in cases with mild and moderate inflammatory changes, followed by a slight decrease in cases with severe inflammation. We have shown a particular association between MCs and LVs that may support the contribution of MCs to the development of the lymphatic vasculature in inflammatory conditions. MCD correlated with LMVD in all cases with mild and moderate inflammatory changes, but not in cases with severe inflammation. No correlation was found between MCD/LMVD and the density of the inflammatory infiltrate. Our results suggest the potential involvement of MCs in the induction and maintenance of lymphangiogenesis in the gingiva of patients with periodontal disease in the early steps of evolution.

Cimpean A.M.,Angiogenesis Research Center Timisoara | Balica R.A.,Angiogenesis Research Center Timisoara | Doros I.C.,Victor Babes University of Medicine and Pharmacy Timisoara | Balica N.C.,Victor Babes University of Medicine and Pharmacy Timisoara | And 3 more authors.
Cancer Genomics and Proteomics | Year: 2016

Molecular classifications of several malignancies are already accepted and applied in clinical practice. For head and neck squamous cell carcinomas (HNSCCs) there exist few and controversial data regarding their stratification on distinct groups or sub-groups and thus, none of them are validated as useful tools for diagnosis and therapy. Starting from the highly expressed markers in HNSCC (epidermal growth factor receptor, keratin 5 and E cadherin) we proposed to identify distinct HNSCC sub-groups with a potential impact on prognosis and therapy. Complex analysis of immunohistochemical expression for six surrogate markers (EGFR, p53, Bcl2, CD117, keratin 5 and E-cadherin) defined three distinct sub-classes amongst EGFR-positive cases, based on the association and differential expression of p53 and Bcl2 (EGFR+/p53-/bcl2-, EGFR+/p53+/bcl2- and EGFR+/p53+/bcl2+). Amongst them, only the EGFR+/p53+/bcl2- sub-class showed significant correlations with grade and TNM parameters. Keratin 5-positive cases were grouped in a special "basal like" group with a particular sub-class rich in CD117+/p63+ cells also highly expressing EGFR. Presence of K5+/CD117+/p63+ cells was correlated with all TNM staging parameters defining a particular sub-class with high aggressiveness and particular behavior. Our data sustain EGFR as the key player in the pathogenesis of HNSCCs, but its diagnostic value may be improved by association with other prognostic or therapeutic markers. We herein defined two distinct HNSCCs groups (EGFR+ and K5+) with several sub-classes, identifiable by the additional assessment of p53, Bcl2 and CD117.

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