Woo J.H.,Texas A&M University |
Frankel A.,Texas A&M University |
Neville Jr. D.M.,Angimmune LLC
Drugs of the Future | Year: 2010
Diphtheria toxin (DT) fusion proteins can selectively target malignant cells and induce cell death by a different mechanism than conventional chemotherapeutic drugs. Cytotoxic therapy with DT fusion proteins is a potential treatment option for relapsed/refractory cancers that are resistant to chemotherapeutic drugs. Due to the heterogeneity of chemical conjugates, the majority of DT fusion proteins used in clinical trials are recombinant DT fusion proteins. These consist of the catalytic and translocation domains of DT fused to tumor-selective ligands (single-chain antibody fragments [sFv], cytokines and growth factors). In this review, recent progress in DT fusion protein design and synthesis, clinical updates of DT fusion protein trials, and the challenges and perspectives for DT fusion proteins are discussed. Although DT fusion proteins have a unique mechanism of action toward tumor cells, their clinical use is limited by nonspecific cell toxicity, particularly endothelial damage. Immunogenicity also limits repeated dosing. New approaches to overcome these limitations are needed. Copyright © 2010 Prous Science, S.A.U. or its licensors. All rights reserved. Source
Zhu W.,South-Central University for Nationalities |
Liu Y.,South-Central University for Nationalities |
Cao X.,South-Central University for Nationalities |
Zhang S.,South-Central University for Nationalities |
And 3 more authors.
Journal of Environmental Management | Year: 2015
Water pollution causes substantial damage to the environment and to human health, and the current methods to treat pollution suffer from high cost and low efficiency, resulting in increased environmental damages. Using genetic modification and functional selection, we developed a novel biosorbent from Genetically Engineered Bacillus subtilis (GEBS) cells. At a ratio of biosorbent to direct blue dye of about 1:1.25 in a water solution, the dye pigments can be completely adsorbed in 40 s, decreasing COD to zero. Contrary to other biosorbents, ions such as Fe2+ and Cu2+ have significant advantages in terms of the adsorbing efficiency. The GEBS biomass can therefore capture both organics and ions from wastewater simultaneously and achieve co-precipitation in 2-10 min, which are features critical for practical applications of wastewater treatment. In addition, we used six different eluting solutions to regenerate used biomass, all resulting in renewed, highly efficient color and COD elimination capacities, with the best elution solution being NaHCO3 and Na2CO3. For practical applications, we showed a high COD elimination rate when using the GEBS biomass to treat raw water from textile enterprises, paper mill, and petrochemical industries. Compared with currently available adsorbing agents, the GEBS cells can adsorb organic and ion waste much faster and with much higher efficiency, can be regenerated and recycled efficiently, and may have broad applications in treating organic water pollution. © 2015 Elsevier Ltd. Source
Angimmune LLC | Date: 2011-10-06
Angimmune LLC | Date: 2012-02-20
Angimmune Llc | Date: 2013-03-13
Methods of modulating the immune systems of patients suffering from cancers that do not hear, or do not uniformly bear, surface CD3 are provided. The methods involve administering an anti-CD3 immunotoxin (e.g. A-dmDT390-bisFv(UCHT1)), to the patient so a to cause the patients Immune system to recognize and destroy non-CD3 cancer cells.