Andong, South Korea
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Choi S.W.,National Research Laboratory for Mitochondrial Signaling | Ahn J.S.,National Research Laboratory for Mitochondrial Signaling | Kim H.K.,National Research Laboratory for Mitochondrial Signaling | Kim N.,National Research Laboratory for Mitochondrial Signaling | And 6 more authors.
Korean Journal of Physiology and Pharmacology | Year: 2011

ATP-sensitive K+ channels (KATP) are major component of preventing ischemia-reperfusion injury. However, there is little information regarding to the expressional difference of KATP and its function between left and right ventricles. In this study, we measured the lactate dehydrogenase release of rabbit heart slices in vitro and determined the difference of the KATP expression at the both ventricles by measuring the level of KATP-forming Kir6.2 (OcKir6.2) mRNA using in situ hybridization. The hearts were preconditioned with 15 min hypoxia and reoxygenated for 15 min before a hypoxic period of 60 min, followed by reoxygenation for 180 min. With hypoxic preconditioning (100% N2) with 15 min, left ventricles (LV) showed higher release of LDH comparing with right ventricles (RV). Adding KATP blocker glibenclamide (10 μ M) prior to a hypoxic period of 60 min, hypoxic preconditioning effect of RV was more abolished than LV. With in situ hybridization, the optical density of OcKir6.2 was higher in RV. Therefore, we suggest that different KATP expression between LV and RV is responsible for the different response to hypoxia and hypoxic preconditioning of rabbit hearts.


Park W.S.,Inje University | Ko J.-H.,Chung - Ang University | Ko E.A.,Inje University | Son Y.K.,University Paris - Sud | And 6 more authors.
Journal of Pharmacological Sciences | Year: 2010

We investigated the effects of YC-1, an activator of soluble guanylyl cyclase (sGC), on voltage-dependent K+ (Kv) channels in smooth muscle cells from freshly isolated rabbit coronary arteries by using the whole-cell patch clamp technique. YC-1 inhibited the Kv current in a dose-dependent fashion with an apparent Kd of 9.67 μM. It accelerated the decay rate of Kv channel inactivation without altering the kinetics of current activation. The rate constants of association and dissociation for YC-1 were 0.36 ± 0.01 μM-1·s-1 and 3.44 ± 0.22 s-1 , respectively. YC-1 did not have a significant effect on the steady-state activation and inactivation curves. The recovery time constant from inactivation was decreased in the presence of YC-1, and application of train pulses (1 or 2 Hz) caused a progressive increase in the YC-1 blockade, indicating that YC-1-induced inhibition of Kv currents is use-dependent. Pretreatment with Bay 41-2272 (also a sGC activator), ODQ (a sGC inhibitor), or Rp-8-Br-PET-cGMPs (a protein kinase G inhibitor) did not affect the basal Kv current and also did not significantly alter the inhibitory effect of YC-1. From these results, we suggest that YC-1 directly inhibits the Kv current independently of sGC activation and in a state-, time-, and use-dependent fashion. ©2010 The Japanese Pharmacological Society.


Kim H.K.,Inje University | Kang S.W.,Inje University | Jeong S.H.,Inje University | Kim N.,Inje University | And 10 more authors.
Journal of Cardiology | Year: 2012

Background: Ischemic preconditioning (IPC) is a powerful mechanism for limiting myocardial infarction during or after ischemia-reperfusion (IR) injury. However, effective target genes and proteins for IPC are unknown. We characterized global changes in gene expression in the heart during IR, and identified effective target genes for IPC. Methods: Hearts were isolated from Sprague-Dawley rats under control, IR, and IPC conditions. We generated expressed-sequence-tags (ESTs) for each group and investigated their functions and the major biological processes in which they are involved using the eukaryotic clusters of orthologous groups (KOG) database and bioinformatics analysis tools. Results: IR modified the expression of 126 genes. Of these, 62 were upregulated, 64 were downregulated, and 77 were found to be effective target genes for IPC. In KOG analysis, most of the genes whose expression was modified were involved in energy production and conversion and the cytoskeleton. A gene-to-gene interaction map revealed that IR modified the expression of genes in four major functional modules: electron transport chain/oxidative phosphorylation; tricarboxylic acid cycle/glucose metabolism/amino acid metabolism; cellular structure and contraction; and gene transcription, translation, and protein folding. At the individual gene level, the genes encoding mitochondrial cytochrome c oxidase subunits 2 and 3 were downregulated, and those encoding the major cytoskeleton components tropomyosin, myosin light chain, myomesin 2, and myosin regulatory light chain 2, as well as the gene encoding the iron-storage protein ferritin, were upregulated, and thus were identified as potential target genes. Real time PCR evaluated expression patterns of three mitochondrial IPC effective genes. Two-dimensional electrophoresis proteomic analyses revealed altered expression of 14 target proteins. The expression patterns of six proteins matched the corresponding EST expression patterns. Conclusion: The global profiling of cardiac ischemia-related genes provides the possible mechanisms of IR and IPC and ways of treating IR injury. © 2012 Japanese College of Cardiology.


Seo D.Y.,Inje University | Lee S.,Inje University | Figueroa A.,Florida State University | Kim H.K.,Inje University | And 9 more authors.
Korean Journal of Physiology and Pharmacology | Year: 2012

Yoga has been known to have stimulatory or inhibitory effects on the metabolic parameters and to be uncomplicated therapy for obesity. The purpose of the present study was to test the effect of an 8-week of yoga-asana training on body composition, lipid profile, and insulin resistance (IR) in obese adolescent boys. Twenty volunteers with body mass index (BMI) greater than the 95th percentile were randomly assigned to yoga (age 14.7±0.5 years, n=10) and control groups (age 14.6±1.0 years, n=10). The yoga group performed exercises three times per week at 40-60% of heart-rate reserve (HRR) for 8 weeks. IR was determined with the homeostasis model assessment of insulin resistance (HOMA-IR). After yoga training, body weight, BMI, fat mass (FM), and body fat % (BF %) were significantly decreased, and fat-free mass and basal metabolic rate were significantly increased than baseline values. FM and BF % were significantly improved in the yoga group compared with the control group (p<0.05). Total cholesterol (TC) was significantly decreased in the yoga group (p<0.01). HDL-cholesterol was decreased in both groups (p<0.05). No significant changes were observed between or within groups for triglycerides, LDL-cholesterol, glucose, insulin, and HOMA-IR. Our findings show that an 8-week of yoga training improves body composition and TC levels in obese adolescent boys, suggesting that yoga training may be effective in controlling some metabolic syndrome factors in obese adolescent boys.


Park W.S.,Kangwon National University | Son Y.K.,Kangwon National University | Ko E.A.,Inje University | Choi S.W.,Inje University | And 6 more authors.
Korean Journal of Physiology and Pharmacology | Year: 2010

We investigated the effects of a hot-water extract of Artemisia iwayomogi, a plant belonging to family Compositae, on cardiac ventricular delayed rectifier K+ current (IK) using the patch clamp technique. The carbohydrate fraction AIP1 dose-dependently increased the heart rate with an apparent EC50 value of 56.1±5.5 μg/ml. Application of AIP1 reduced the action potential duration (APD) in concentration-dependent fashion by activating IK without significantly altering the resting membrane potential (IC50 value of APD50: 54.80±2.24, IC 50 value of APD90: 57.45±3.47 μg/ml). Based on the results, all experiments were performed with 50 μg/ml of AIP1. Pre-treatment with the rapidly activating delayed rectifier K+ current (IKr) inhibitor, E-4031 prolonged APD. However, additional application of AIP1 did not reduce APD. The inhibition of slowly activating delayed rectifier K+ current (IKs) by chromanol 293B did not change the effect of AIP1. AIP1 did not significantly affect coronary arterial tone or ion channels, even at the highest concentration of AIP1. In summary, AIP1 reduces APD by activating IKr but not IKs. These results suggest that the natural product AIP1 may provide an adjunctive therapy of long QT syndrome.


Kim H.K.,Inje University | Park W.S.,Kangwon National University | Warda M.,Cairo University | Park S.Y.,Pusan National University | And 11 more authors.
PLoS ONE | Year: 2012

Background and Purpose: Beta adrenergic overstimulation may increase the vascular damage and stroke. However, the underlying mechanisms of beta adrenergic overstimulation in cerebrovascular dysfunctions are not well known. We investigated the possible cerebrovascular dysfunction response to isoproterenol induced beta-adrenergic overstimulation (ISO) in rabbit cerebral arteries (CAs). Methods: ISO was induced in six weeks aged male New Zealand white rabbit (0.8-1.0 kg) by 7-days isoproterenol injection (300 μg/kg/day). We investigated the alteration of protein expression in ISO treated CAs using 2DE proteomics and western blot analysis. Systemic properties of 2DE proteomics result were analyzed using bioinformatics software. ROS generation and following DNA damage were assessed to evaluate deteriorative effect of ISO on CAs. Intracellular Ca2+ level change and vascular contractile response to vasoactive drug, angiotensin II (Ang II), were assessed to evaluate functional alteration of ISO treated CAs. Ang II-induced ROS generation was assessed to evaluated involvement of ROS generation in CA contractility. Results: Proteomic analysis revealed remarkably decreased expression of cytoskeleton organizing proteins (e.g. actin related protein 1A and 2, α-actin, capping protein Z beta, and vimentin) and anti-oxidative stress proteins (e.g. heat shock protein 9A and stress-induced-phosphoprotein 1) in ISO-CAs. As a cause of dysregulation of actin-cytoskeleton organization, we found decreased level of RhoA and ROCK1, which are major regulators of actin-cytoskeleton organization. As functional consequences of proteomic alteration, we found the decreased transient Ca2+ efflux and constriction response to angiotensin II and high K+ in ISO-CAs. ISO also increased basal ROS generation and induced oxidative damage in CA; however, it decreased the Ang II-induced ROS generation rate. These results indicate that ISO disrupted actin cytoskeleton proteome network through down-regulation of RhoA/ROCK1 proteins and increased oxidative damage, which consequently led to contractile dysfunction in CA. © 2012 Kim et al.


Kim Y.M.,University of Suwon | Yoo E.K.,Hanyang University | Song M.K.,Andong Science College andong
International Journal of Bio-Science and Bio-Technology | Year: 2016

The purpose of this study was to develop nurse-led home visit intervention program in order to maintain and promote holistic healthcare of multi-cultural couples in farm and fishery areas. The period of research was from March 2014 to July 2014. The method was literature reviews and interviews. Firstly, through literature reviews and secondly, interviews with multi-cultural couples, the health problems and educational demands of subjects were found. Based on the findings, Nurse-led Home Visit Intervention Program was developed. The content of the program was composed of health examination and education, counseling and support, relaxation therapy, and prayer from a holistic healthcare perspective providing through home visitations and phone counseling once a week for one hour at a time under the topics of ‘healthy couples’, ‘wise and frugal couples’, ‘assimilating couples’, and ‘happy couples’ as six sessions. And the program operation method was applying the JUMP (Joy Ultra Mom Program) operation skill, which facilitates interaction and reciprocal communication among participants, such as couples, learning from each other, opening their heart, and understanding each other. It is necessary to apply and test the effects on the holistic health of multi-cultural couples at related institutes such as multi-cultural centers and health centers, and it is also necessary to continuously develop customized health programs according to their cultural differences. © 2016 SERSC.


Woo Y.,Jeonju University | Lee J.,Andong Science College andong | Hwang S.,Baekseok University | Hong C.P.,Yonsei University
Journal of the Korean Physical Society | Year: 2013

The purpose of this study was to investigate the associations between gait performance, postural stability, and depression in patients with Parkinson's disease (PD) by using an adaptive neuro-fuzzy inference system (ANFIS). Twenty-two idiopathic PD patients were assessed during outpatient physical therapy by using three clinical tests: the Berg balance scale (BBS), Dynamic gait index (DGI), and Geriatric depression scale (GDS). Scores were determined from clinical observation and patient interviews, and associations among gait performance, postural stability, and depression in this PD population were evaluated. The DGI showed significant positive correlation with the BBS scores, and negative correlation with the GDS score. We assessed the relationship between the BBS score and the DGI results by using a multiple regression analysis. In this case, the GDS score was not significantly associated with the DGI, but the BBS and DGI results were. Strikingly, the ANFIS-estimated value of the DGI, based on the BBS and the GDS scores, significantly correlated with the walking ability determined by using the DGI in patients with Parkinson's disease. These findings suggest that the ANFIS techniques effectively reflect and explain the multidirectional phenomena or conditions of gait performance in patients with PD. © 2013 The Korean Physical Society.


Chung E.,Andong Science College andong | Lee B.-H.,Sahmyook University | Hwang S.,Baekseok University
Restorative Neurology and Neuroscience | Year: 2014

Objective: The purpose of this study was to examine the feasibility of core stabilization exercise with real-time feedback on balance and gait function in patients with chronic hemiparetic stroke. Methods: Nineteen stroke subjects were enrolled in this study. The patients were randomly divided into the experimental (n = 10) and control groups (n = 9). Subjects in the experimental group performed core stabilization exercise with real-time feedback training for 30 minutes per day during a period of six weeks. Subjects in the control group performed core stabilization exercise during the same period. This study assessed the kinematic parameters using a portable walkway system, and timed up-and-go test. Results: Gait velocity showed significantly greater improvement in the experimental group (7.3 ± 5.0 sec) than in the control group (-0.7 ± 10.6). Stride length showed significantly greater increase in the experimental group (13.2 ± 7.9 on the affected side and 12.6 ± 8.0 on the less affected side) than the control group (3.5 ± 8.7 on the affected side and 3.4 ± 8.5 on the less affected side). After training, change in results on the timed up and go test was significantly greater in the experimental group than in the control group. Conclusion: Core stabilization exercise using real-time feedback produces greater improvement in gait performance in chronic hemiparetic stroke patients than core stabilization exercise only. © 2014 - IOS Press.

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