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Subbaiah E.V.,DNA Diagnostics Center | Royer C.,INSA Lyon | Kanginakudru S.,DNA Diagnostics Center | Satyavathi V.V.,DNA Diagnostics Center | And 9 more authors.
Genetics | Year: 2013

Bombyx mori nucleopolyhedrovirus (BmNPV) that infects the silkworm, B. mori, accounts for > of silk cocoon crop losses globally. We speculated that simultaneous targeting of several BmNPV essential genes in transgenic silkworm would elicit a stable defense against the virus. We introduced into the silkworm germline the vectors carrying short sequences of four essential BmNPV genes in tandem, either in sense or antisense or in inverted-repeat arrangement. The transgenic silkworms carrying the inverted repeat-containing transgene showed stable protection against high doses of baculovirus infection. Further, the antiviral trait was incorporated to a commercially productive silkworm strain highly susceptible to BmNPV. This led to combining the high-yielding cocoon and silk traits of the parental commercial strain and a very high level of refractoriness (>75% survival rate as compared to,<15% in nontransgenic lines) to baculovirus infection conferred by the transgene. We also observed impaired infectivity of the occlusion bodies derived from the transgenic lines as compared to the wild-type ones. Currently, large-scale exploitation of these transgenic lines is underway to bring about economic transformation of sericulture. © 2013 by the Genetics Society of America.

Ramesha C.,Andhra Pradesh State Sericulture Research and Development Institute | Anuradha C.M.,Sri Krishnadevaraya University | Lakshmi H.,Andhra Pradesh State Sericulture Research and Development Institute | Kumari S.S.,Indian Institute of Chemical Technology | And 3 more authors.
Biotechnology | Year: 2010

In order to amalgamate the effects of nutrition and its efficiency on genetic traits expression in silkworm germplasm breeds by serial analysis on nutrigenetic traits and utilized as biomarker with an aim to identify nutritionally efficient silkworm germplasm breeds. In the present study, six bivoltine silkworm breeds were subjected for investigation on ingestion, digestion and utilization of dry food matter in single silkworm larva and its sharing on economically important stages larva, cocoon and shell weight in 5th stage. It was resolved that low consumption with high conversion efficiency of food ingested in silkworm breeds on nineteen nutrigenetic traits analyzed by standard gravimetric method with the aid of INDOSTAT software to understand the nutrigenomic divergence among the silkworm germplasm breeds against different seasons. Results indicated that higher conversion efficiency was observed in most of the new silkworm breeds for Efficiency of Conversion of Ingesta (ECI) to cocoon and shell than control (APS ) as leaf - cocoon and leaf-shell conversion 8 are the ultimate indices in nutrigenomic analysis. It also demonstrated that relatively smaller amount of Consumption Index (CI), respiration, Metabolic Rate (MR) with superior Relative Growth Rate (RGR) and in relation to quantum of food ingesta and digesta requisite per gram of cocoon and shell found less than the control. This concluded that nutrigenetic traits directly associated to the quantum of food ingesta and digesta g•1 of cocoon and shell and such nutrigenetic study pragmatically utilized as biomarkers in order to advocate sturdily the following breeds viz., RBD1 , RBD4 (peanut type cocoon), RBO2 , RBO3 (oval type cocoon) were chosen as highly nutritionally efficient bivoltine silkworm germplasm breeds. © 2010 Asian Network for Scientific Information.

Kumar K.P.K.,Central Tasar Research and Training Institute | Seshagiri S.V.,Andhra Pradesh State Sericulture Research and Development Institute | Ramesha C.,Andhra Pradesh State Sericulture Research and Development Institute | Basha K.I.,Andhra Pradesh State Sericulture Research and Development Institute
Indian Silk | Year: 2010

Many a good technologies intended to enhance the quality and productivity at various stages of silk production/ processing, often fall short of expectations, due to lack of awareness about them among the end-users. The present study was conducted in the traditional silk producing area with the high concentration of silk reelers in Anantapur district of Andhra Pradesh. The data was collected from each respondent through a question- naire during 2007 - 08 on the following aspects, eliciting their opinions on various managerial aspects of reeling technologies. The study found that 30% of charka reelers were aware of filatures, 48% of multiend, 30% of cottage basin and 100% of traditional charka technologies. The data revealed that adoption rate of cocoon and raw silk quality testing methods was completely nil with all the respondents. On the other hand, 80% of cottage basin reelers followed optimum time and temperature parameters.

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