Anatomopathology Laboratory

Sfax, Tunisia

Anatomopathology Laboratory

Sfax, Tunisia
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Ghlissi Z.,University of Sfax | Hakim A.,University of Sfax | Sila A.,University of Sfax | Mnif H.,Anatomopathology Laboratory | And 4 more authors.
Environmental Toxicology and Pharmacology | Year: 2014

Objective: We evaluated the effect of astaxanthin (ASX) and vitamin E (vit E) on colistin methanesulfonate (CMS) induced-nephrotoxicity in rats. Methods: Animals were treated with sterile saline, 300. 000 or 450. 000. IU/kg/day of CMS, CMS. +. ASX (20. mg/kg), CMS. +. vit E (100. mg/kg), or CMS. +. 1. ml/kg olive oil (OO) for 7 days. The plasma/urine creatinine (Cr) level, urine γ-glutamyl-transferase (GGT) level, and renal tissue activities in malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and reductase (GSH), as well as renal histology were performed. Results: CMS induced a tubular damage, increased the GGT and MDA levels, and decreased the activities of SOD, CAT, GPx and GSH. Co-treatment with ASX or vit E restored all biochemical parameters cited above and improved the histopathological damage. Conclusion: Nephrotoxicity induced by CMS might be due to oxidative damage. The improvement by ASX or vit E seems to be related to their antioxidant properties. © 2014 Elsevier B.V.

Soudani N.,University of Sfax | Troudi A.,University of Sfax | Bouaziz H.,University of Sfax | Ben Amara I.,University of Sfax | And 2 more authors.
Ecotoxicology and Environmental Safety | Year: 2011

Acute exposure to hexavalent chromium compounds can cause cardiotoxicity. Our study pertains to the protective effect of selenium against K2Cr2O7-induced cardiotoxicity. Female Wistar rats were divided into four groups of six each: group I served as controls which received standard diet; group II received in drinking water K2Cr2O7 alone (700ppm); group III received both K2Cr2O7 and Se (0.5 Na2SeO3mg/kg of diet); group IV received Se (0.5mg/kg of diet) for 3 weeks. The exposure of rats to chromium promoted oxidative stress with an increase in malondialdehyde levels and a decrease in antioxidant non-enzymatic levels such as glutathione, non-protein thiol and vitamin C, while, an increase in glutathione peroxidase, superoxide dismutase and catalase activities was observed. However, plasma transaminases, lactate dehydrogenase activities, cholesterol, triglycerides and low density lipoprotein-cholesterol levels increased, and high density lipoprotein-cholesterol decreased. Coadministration of Se restored the parameters cited above to near-normal values. The histopathological findings confirmed the biochemical results. © 2010 Elsevier Inc.

Ghorbel I.,Animal Physiology Laboratory | Chaabane M.,Animal Physiology Laboratory | Elwej A.,Animal Physiology Laboratory | Boudawara O.,Anatomopathology Laboratory | And 4 more authors.
Archives of Physiology and Biochemistry | Year: 2016

Hepatotoxicity, induced by aluminium chloride (AlCl3), has been well studied but there are no reports about liver metallothionein (MT) genes induction. Therefore, it is of interest to establish the mechanism involving the relation between MT gene expression levels and the oxidative stress status in hepatic cells of aluminium-treated rats. Aluminium (Al) was administered to rats in their drinking water at a dose of 50 mg/kg body weight for three weeks. AlCl3 provoked hepatotoxicity objectified by an increase in malondialdehyde (MDA), hydrogen peroxide (H2O2), advanced oxidation protein products (AOPP), protein carbonyls (PCO) and a decrease in reduced glutathione (GSH), non-protein thiols (NPSH) and vitamin C. CAT and Glutathione peroxidase (GPx) activities were decreased while Mn-SOD gene expression, total Metallothionein content and MT I and MT II genes induction were increased. There are changes in plasma of some trace elements, albumin levels, transaminases, LDH and ALP activities. All these changes were supported by histopathological observations. © 2016 Informa UK Limited, trading as Taylor & Francis Group.

Ben Amara I.,University of Sfax | Troudi A.,University of Sfax | Garoui E.,University of Sfax | Hakim A.,University of Sfax | And 3 more authors.
Experimental and Toxicologic Pathology | Year: 2011

This study aims to investigate the improving effects of selenium on methimazole-induced kidney impairments in adult rats and their pups. The animals were randomly divided into four groups of six each: group I served as control which received standard diet; group II received only methimazole in drinking water as 250mg/l; group III received both methimazole (250mg/l, orally) and selenium (0.5mg/kg of diet); group IV served as a positive control and received selenium (0.5mg/kg of diet) as sodium selenite (Na 2SeO 3). Treatments were started from the 14th day of pregnancy until day 14 after delivery. In the methimazole-treated group, body and absolute kidney weights decreased in pups and their mothers when compared to control. Daily urine volume, plasma creatinine levels were higher, while urinary levels were lower than in control. Besides, antioxidant enzyme activities, superoxide dismutase, catalase and glutathione peroxidase decreased. Lipid peroxidation recorded an increase revealed by high kidney malondialdehyde levels, while those of plasma and urinary uric acid showed a significant decline. Methimazole-treated rat kidneys exhibited leucocytic infiltrations, vascular congestion and narrowed Bowman's space. Co-administration of selenium through diet improved all the parameters cited above in adult rats and their progeny. Nevertheless, the distorted histoarchitecture in rat kidney was alleviated by selenium treatment. It can then be concluded that selenium is an important protective element that may be used as a dietary supplement against kidney impairments. © 2010 Elsevier GmbH.

Rivoirard R.,Institute Of Cancerologie Lucien Neuwirth | Chargari C.,Hopital DInstruction des Armees du Val de Grace | Guy J.-B.,Institute Of Cancerologie Lucien Neuwirth | Peoc'h M.,Anatomopathology Laboratory | And 10 more authors.
Chemotherapy | Year: 2016

Objectives: Glioblastoma is one of the most frequent primitive brain tumors. Patients who experience tumor relapse after surgery and concomitant radiochemotherapy have a dismal prognosis. The objective of this study is to analyze efficacy data in terms of overall survival (OS) and progression- free survival (PFS) following combination therapy with bevacizumab (BVZ) and irinotecan among patients with relapsed glioblastoma. Safety data will also be reviewed and all results will be compared with data of the literature. Methods: In this single-center retrospective study, all records of patients treated with BVZ and irinotecan for a relapsed glioblastoma were analyzed. Each chemotherapy cycle was repeated every 15 days until progression. Magnetic resonance imaging and neurologic examination were repeated every 6 weeks during treatment. Results: Forty-five patients were analyzed. The median number of BVZ-irinotecan cycles was 8 (range 1-38). Median PFS was 26 weeks and median OS was 28 weeks. Eighteen of the 45 patients (40% of cases) had an objective response 6 months after initiation of treatment. Two patients had to discontinue treatment due to toxicity. Conclusions: The results of the SV1 study are consistent with those found in phase II studies evaluating the same treatment. The irinotecan-BVZ combination is effective in relapsed glioblastoma with acceptable toxicity. Biomarkers predictive of response to BVZ should help in the selection of patients who could benefit from treatment. © 2016 S. Karger AG, Basel.

Soudani N.,University of Sfax | Sefi M.,University of Sfax | Bouaziz H.,University of Sfax | Chtourou Y.,University of Sfax | And 2 more authors.
Human and Experimental Toxicology | Year: 2011

To assess kidney damages in pregnant and lactating rats and in their suckling pups, Wistar female rats were given, through drinking water, 700 parts per million (ppm) of K2Cr2O7 from the 14th day of pregnancy until day 14 after delivery. Toxicity was objectified by a significant increase in malondialdehyde (MDA), glutathione (GSH) and nitric oxide (NO) levels in kidney of chromium-treated mothers and their suckling pups. Moreover, lactate dehydrogenase (LDH) was increased in kidney and decreased in plasma of K2Cr2O7-treated rats. Activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) were increased in dams and decreased in their pups. Interestingly, these biochemical modifications were accompanied by higher plasma and lower urinary levels of creatinine, a specific indicator of glomerular function, and of urea than those of controls. Significant increase in creatinine clearance was also found in treated mothers and in their progeny. Histological studies showed an infiltration of mononuclear cells, necrosis and vascular congestion in kidney of pups and dams. Based on the present findings, K2Cr2O7 administrated to female rats during late pregnancy and early postnatal periods provoked kidney damages in dams and their offspring. © 2011 SAGE Publications.

Boukettaya W.,CHU Habib Bourguiba | Feki J.,CHU Habib Bourguiba | Charfi S.,Anatomopathology Laboratory | Toumi N.,CHU Habib Bourguiba | And 4 more authors.
Urology Case Reports | Year: 2014

Primary signet ring cell carcinoma of the urinary bladder is a rare and aggressive histologic subtype of adenocarcinoma. In general, this tumor occurs in the middle age, and clinical presentation does not differ from transitional cell carcinomas. The prognosis is often poor, given the advanced stage at diagnosis. To our knowledge, <100 cases of signet ring cell adenocarcinoma of the urinary bladder have been reported. We report 2 cases with bladder linitis plastica primitive, and we draw attention to its pathologic, anatomoclinical, and evolution specificity to optimize its therapeutic management. © 2014 The Authors.

Soudani N.,Animal Physiology Laboratory | Sefi M.,Animal Physiology Laboratory | Ben Amara I.,Animal Physiology Laboratory | Boudawara T.,Anatomopathology Laboratory | Zeghal N.,Animal Physiology Laboratory
Ecotoxicology and Environmental Safety | Year: 2010

Chromium is a toxic metal implicated in human diseases. This study was focused on investigating the possible protective effect of Se against K2Cr2O7. Female Wistar rats, used in this study, were divided into four groups of six animals each: group I served as control which received standard diet; group II received orally only K2Cr2O7 (700ppm equivalent to 67mg/kgbw); group III received both K2Cr2O7 and Se (0.5mg/kg of diet); group IV received Se (0.5mg Na2SeO3/kg of diet). The exposure of rats to K2Cr2O7 for 21 days provoked renal damages with a significant increase in kidney malondialdehyde, superoxide dismutase, plasma creatinine, and uric acid levels, while catalase, glutathione peroxidase, non-protein thiol, Metallothionein and plasma urea levels decreased. Coadministration of Se in the diet of chromium-treated group improved malondialdehyde, renal biomarkers levels and antioxidant enzyme activities. Kidney histological studies confirmed biochemical parameters and the beneficial role of selenium. © 2009 Elsevier Inc.

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