Dolores Hidalgo Cuna de la Independencia Nacional, Mexico
Dolores Hidalgo Cuna de la Independencia Nacional, Mexico

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Nunez-Sanchez M.A.,CSIC - Center of Edafology and Applied Biology of the Segura | Davalos A.,IMDEA Madrid Institute for Advanced Studies | Gonzalez-Sarrias A.,CSIC - Center of Edafology and Applied Biology of the Segura | Casas-Agustench P.,IMDEA Madrid Institute for Advanced Studies | And 13 more authors.
Molecular Nutrition and Food Research | Year: 2015

Scope: MicroRNAs (miRs) are proposed as colorectal cancer (CRC) biomarkers. Pomegranate ellagic acid and their microbiota metabolites urolithins exert anticancer effects in preclinical CRC models, and target normal and malignant colon tissues in CRC patients. Herein, we investigated whether the intake of pomegranate extract (PE) modified miRs expression in surgical colon tissues versus biopsies from CRC patients. Methods and results: We conducted a randomized, double-blind, controlled trial. Thirty-five CRC patients consumed 900 mg PE daily before surgery. Control CRC patients (no PE intake, n = 10) were included. Our results revealed: (1) significant differences for specific miRs between malignant and normal tissues modifiable by the surgical protocols; (2) opposed trends between -5p and -3p isomolecules; (3) general induction of miRs attributable to the surgery; (4) moderate modulation of various miRs following the PE intake, and (5) no association between tissue urolithins and the observed miRs changes. Conclusion: PE consumption appears to affect specific colon tissue miRs but surgery critically alters miRs levels hindering the discrimination of significant changes caused by dietary factors and the establishment of genuine differences between malignant and normal tissues as biomarkers. The components responsible for the PE effects and the clinical relevance of these observations deserve further research. © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.


Soares M.,University of Lisbon | Correia J.,University of Lisbon | Cabecadas J.,Anatomical Pathology Service | Peleteiro C.,University of Lisbon | Ferreira F.,University of Lisbon
Proceedings of the International Workshop on Veterinary Biosignals and Biodevices, VBB 2012, in Conjunction with BIOSTEC 2012 | Year: 2012

The overexpression of the Human Epidermal growth factor Receptor-2 (HER-2) oncogene in human breast cancer is associated with a poor prognosis and a specific treatment. Because of its importance and as a first line option for diagnosis, well established guidelines for its detection are based in immunohistochemical techniques, Still, in Veterinary Medicine there is little and inconsistent information about this subject. The aim of our study was to achieve an optimal immunohistochemical protocol for detection of fHER-2 in Feline Mammary Carcinoma (FMC). Five commercial anti-HER-2 antibodies were tested using three different protocols. The fHER-2 protein overexpression was detected in 10 of the 30 FMC cases (33.3%), when the optimized protocol was performed (associating the A0485 antibody with a longer antigen retrieval method). These results suggest that fHER-2 may play an important role in Feline Oncology and that the Cat can be a suitable animal model for human breast cancer research.


Ventura-Rios L.,Instituto Nacional Of Rehabilitacion | Hernandez-Diaz C.,Instituto Nacional Of Rehabilitacion | Gutierrez-Perez L.,Musculoskeletal Ultrasound Diploma Course | Bernal-Gonzalez A.,Instituto Nacional Of Rehabilitacion | And 3 more authors.
Clinical Rheumatology | Year: 2016

Alkaptonuria is a rare, hereditary metabolic disorder in which a deficiency in the homogentisate 1,2-dioxygenase enzyme results in an accumulation of homogentisic acid. Deposition of excess homogentisic acid in different intra- and extra-articular structures with high content of connective tissue causes brownish-black pigmentation and weakening, ultimately resulting in tissue degeneration and finally osteoarthritis. Ochronotic arthropathy is considered a rapidly progressive, disabling condition in which weight-bearing joints and the thoracolumbar spine are predominantly affected. Patients often require multiple joint replacements, such as in the case of the patient presented here. At present, there is no definitive cure for ochronosis, and management is predominantly symptomatic. © 2014, Clinical Rheumatology.


PubMed | Musculoskeletal Ultrasound Diploma Course, Instituto Nacional Of Rehabilitacion and Anatomical Pathology Service
Type: Journal Article | Journal: Clinical rheumatology | Year: 2016

Alkaptonuria is a rare, hereditary metabolic disorder in which a deficiency in the homogentisate 1,2-dioxygenase enzyme results in an accumulation of homogentisic acid. Deposition of excess homogentisic acid in different intra- and extra-articular structures with high content of connective tissue causes brownish-black pigmentation and weakening, ultimately resulting in tissue degeneration and finally osteoarthritis. Ochronotic arthropathy is considered a rapidly progressive, disabling condition in which weight-bearing joints and the thoracolumbar spine are predominantly affected. Patients often require multiple joint replacements, such as in the case of the patient presented here. At present, there is no definitive cure for ochronosis, and management is predominantly symptomatic.

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