Analytical Section of Attached Hospital of Jining Medical College

Jining, China

Analytical Section of Attached Hospital of Jining Medical College

Jining, China
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Jin C.,Analytical Section of Attached Hospital of Jining Medical College | Dong H.,Analytical Section of Attached Hospital of Jining Medical College | Cheng P.,Analytical Section of Attached Hospital of Jining Medical College | Zhou J.,Analytical Section of Attached Hospital of Jining Medical College | And 2 more authors.
International Immunopharmacology | Year: 2014

The aim of the present study was to investigate the therapeutic effect of halofuginone (HF) in the treatment of idiopathic thrombocytopenic purpura (ITP) and explore the underlying mechanism. Sixty ITP mice were divided into four groups including control group, low dose group (25 mg/kg HF), medium dose group (50 mg/kg HF), and high dose group (100 mg/kg HF). Corresponding dose of HF was administrated by gavage daily in HF groups for 7 days, and the same volume of saline was given in control group. Platelet counts were 28.87 ± 3.91 × 109/L, 57.13 ± 2.75 × 109/L, 86.73 ± 3.06 × 109/L and 89.73 ± 2.84 × 10 9/L in control group, low dose group, medium dose group, and high dose group respectively, on day 7 after intragastrically administration of HF or saline. Compared with control group, three HF groups showed significantly increased levels of INF-γ and IL-2 (all P < 0.05), and significantly decreased concentrations of IL-4 and IL-10(all P < 0.05). The expression of T-bet mRNA increased and the expression of GATA-3 mRNA decreased (all P < 0.05) in ITP mice after intragastric administration with different dose of HF. HF significantly recovered peripheral platelet counts in ITP mice through promoting Th1 cell differentiation and attenuating Th2 differentiation in ITP mice. © 2013 Elsevier B.V.


PubMed | Guangdong Medical College and Analytical Section of Attached Hospital of Jining Medical College
Type: Journal Article | Journal: International immunopharmacology | Year: 2014

The aim of the present study was to investigate the therapeutic effect of halofuginone (HF) in the treatment of idiopathic thrombocytopenic purpura (ITP) and explore the underlying mechanism. Sixty ITP mice were divided into four groups including control group, low dose group (25 mg/kg HF), medium dose group (50 mg/kg HF), and high dose group (100 mg/kg HF). Corresponding dose of HF was administrated by gavage daily in HF groups for 7 days, and the same volume of saline was given in control group. Platelet counts were 28.87 3.91 10(9)/L, 57.13 2.75 10(9)/L, 86.73 3.06 10(9)/L and 89.73 2.84 10(9)/L in control group, low dose group, medium dose group, and high dose group respectively, on day 7 after intragastrically administration of HF or saline. Compared with control group, three HF groups showed significantly increased levels of INF- and IL-2 (all P < 0.05), and significantly decreased concentrations of IL-4 and IL-10(all P < 0.05). The expression of T-bet mRNA increased and the expression of GATA-3 mRNA decreased (all P < 0.05) in ITP mice after intragastric administration with different dose of HF. HF significantly recovered peripheral platelet counts in ITP mice through promoting Th1 cell differentiation and attenuating Th2 differentiation in ITP mice.


Jin C.-Q.,Analytical Section of Attached Hospital of Jining Medical College | Dong H.-X.,Analytical Section of Attached Hospital of Jining Medical College | Zhou J.-W.,Analytical Section of Attached Hospital of Jining Medical College | Jia Y.-X.,Jining No1 Peoples Hospitalshandong Province
Clinica Chimica Acta | Year: 2016

Background Hemophagocytic lymphohistiocytosis (HLH) is a rare, life-threatening disease resulting from excessive activation and non-malignant proliferation of macrophages and T lymphocytes. Whether it can be caused by cholecystitis has not yet been reported in the world. Case report A 4-year-old girl was admitted to hospital with cholecystitis. The patient was diagnosed with hemophagocytic lymphohistiocytosis after 3 days of admission based on the results of laboratory tests showing hypofibrinogenemia, hypertriglyceridemia, thrombocytopenia, anemia and leukopenia. Conclusions From this case experience, if a timely symptomatic treatment is given, the condition of the patient with secondary HLH can be alleviated. This is the first report of cholecystitis-induced hemophagocytic syndrome in the world also. © 2016 Elsevier B.V.


Jin C.-Q.,Analytical Section of Attached Hospital of Jining Medical College | Liu F.,Guangdong Medical College | Dong H.-X.,Analytical Section of Attached Hospital of Jining Medical College | Zhang J.,Analytical Section of Attached Hospital of Jining Medical College | And 4 more authors.
International Journal of Laboratory Hematology | Year: 2012

Introduction: Dysfunctional cellular immunity is considered to be essential to the pathophysiology of Epstein-Barr virus-related idiopathic thrombocytopenic purpura (EBV-ITP). Cytokines in peripheral blood and the gene expression of transcription factors in T lymphocytes of patients with were investigated to correlate the polarization of T helper cell type 1 (Th1)/T helper cell type 2 (Th2) with the degree of thrombocytopenia. Methods: The expression of type 1 (T-bet) and type 2 (GATA-3) in T lymphocytes was detected by semiquantitative reverse-transcription-polymerase chain reaction, and plasma cytokine levels were measured by enzyme-linked immunoassay in 35 patients and 30 control subjects. Results: Th1/Th2 [(interleukin-2+γ-interferon)/(interleukin-10+interleukin-13)] cytokine ratios and transcription factor (T-bet/GATA-3) mRNA ratios were significantly decreased in patients with EBV-ITP, and the Th1/Th2 ratio was directly correlated with platelet counts. Conclusion: Our findings clearly show that type 2 polarization of the autoimmune response accounts for the expression of cytokines and transcription factors in EBV-ITP. © 2011 Blackwell Publishing Ltd.


PubMed | Jining No1 Peoples Hospital and Analytical Section of Attached Hospital of Jining Medical College
Type: | Journal: Clinica chimica acta; international journal of clinical chemistry | Year: 2016

Hemophagocytic lymphohistiocytosis (HLH) is a rare, life-threatening disease resulting from excessive activation and non-malignant proliferation of macrophages and T lymphocytes. Whether it can be caused by cholecystitis has not yet been reported in the world.A 4-year-old girl was admitted to hospital with cholecystitis. The patient was diagnosed with hemophagocytic lymphohistiocytosis after 3days of admission based on the results of laboratory tests showing hypofibrinogenemia, hypertriglyceridemia, thrombocytopenia, anemia and leukopenia.From this case experience, if a timely symptomatic treatment is given, the condition of the patient with secondary HLH can be alleviated. This is the first report of cholecystitis-induced hemophagocytic syndrome in the world also.


PubMed | Analytical Section of Attached Hospital of Jining Medical College
Type: Journal Article | Journal: International journal of laboratory hematology | Year: 2012

Dysfunctional cellular immunity is considered to be essential to the pathophysiology of Epstein-Barr virus-related idiopathic thrombocytopenic purpura (EBV-ITP). Cytokines in peripheral blood and the gene expression of transcription factors in T lymphocytes of patients with were investigated to correlate the polarization of T helper cell type 1 (Th1)/T helper cell type 2 (Th2) with the degree of thrombocytopenia.The expression of type 1 (T-bet) and type 2 (GATA-3) in T lymphocytes was detected by semiquantitative reverse-transcription-polymerase chain reaction, and plasma cytokine levels were measured by enzyme-linked immunoassay in 35 patients and 30 control subjects.Th1/Th2 [(interleukin-2 + -interferon)/(interleukin-10 + interleukin-13)] cytokine ratios and transcription factor (T-bet/GATA-3) mRNA ratios were significantly decreased in patients with EBV-ITP, and the Th1/Th2 ratio was directly correlated with platelet counts.Our findings clearly show that type 2 polarization of the autoimmune response accounts for the expression of cytokines and transcription factors in EBV-ITP.

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