Analytical Research Laboratory

Navi Mumbai, India

Analytical Research Laboratory

Navi Mumbai, India
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Kadam N.N.,Kvpendharkar College | Patil P.C.,Kvpendharkar College | Singh R.R.,Analytical Research Laboratory
International Journal of Pharmacy and Pharmaceutical Sciences | Year: 2011

A simple, specific, accurate and stability-indicating reversed phase high performance liquid chromatographic method was developed for the determination of Camylofin Dihydrochloride in drug substance, using Zorbax eclipse-XDB C18 column (150mm × 4.6 mm, 5 μm) and a mobile phase composed of 0.05% trifluoro acetic acid in water (Mobile phase A) and 0.05% trifluoro acetic acid in acetonitrile (Mobile phase B), at a flow rate of 1.0 mL min-1 using gradient program and UV detection at 220 nm. The Retention time of Phenyl glycine, Phenyl glycine isoamyl ester and Camylofin Dihydrochloride were found to be 1.841 min, 17.524 min and 18.663 min respectively. The proposed method was validated as per ICH guidelines. The Linearity for Camylofin Dihydrochloride was in the range of 250-750 μg mL-1. The recovery was found to be in the range of 99.7-100.2%. Camylofin Dihydrochloride was subjected to acid, alkali and neutral hydrolysis, chemical oxidation and dry environmental condition. The degraded product peaks were well resolved from the pure drug peak with significant difference in their retention time values. This method can be successfully employed for quantitative analysis of Camylofin Dihydrochloride in drug substance.


Masih A.,Analytical Research Laboratory | Verma P.,Envirotech Online Equipment LaboratoryHyderabad | Lal J.K.,Analytical Research Laboratory | Taneja A.,Dr. B.R. Ambedkar University
Advanced Science Letters | Year: 2014

Deepawali—the festival of light—is celebrated in India, every year during October or November with great fireworks display. Fireworks display during festive celebrations can cause acute short term air as well as noise pollution. Concentration of air pollutants such as SO2, NO2, CO, O3, benzene and respirable suspended particulate matter (RSPM) along with noise levels were monitored for five consecutive days during Deepawali at Agra, a densely populated residential area near New Delhi, India, for assessing the impacts of fireworks on ambient air quality. The average total concentrations of SO2, NO2, CO, O3, benzene and RSPM for the entire study period was found to be 14.7, 41.1, 1.9, 10.5, 0.8 and 293.5 μg/m3 respectively. The average total noise level was found to be 72.71 dB. The pollutant concentrations as recorded on Deepawali were found to be slightly higher (1.5 times for SO2, 1.4 times for NO2, 1.7 times for CO, 1.6 times for ozone, 1.4 times for benzene and 1.8 times for RSPM) compared to a typical winter day value. The results indicated the huge contribution of fireworks on the pollutant levels. The temporal variation is similar to other studies conducted in Deepawali suggesting that the problem is rampant in urban India. The peak concentrations of carbon monoxide and respirable suspended particulates exceeded the NAAQS, India 24 hour standard. It suggests some controls on fireworks during festive celebrations. All the pollutants showed similar variation pattern during the monitoring period. The concentrations increased steadily to the peak on Deepawali and declined thereafter. Further studies on control measures for firework display during such celebrations are necessary to reduce the probable health hazards. © 2014 American Scientific Publishers. All rights reserved.


Masih A.,Analytical Research Laboratory | Masih J.,Wilson College | Taneja A.,Dr. M.G.R. Educational and Research Institute
Journal of Environmental Monitoring | Year: 2012

Soil is the major environmental reservoir of organic compounds and soil-air exchange is a key process in governing the environmental fate of these compounds on a regional and global scale. Samples of air and soil were collected to study the levels of PAHs in the air and soil of the Agra region. Concentrations of PAH measured at four locations in the city of Agra, covers industrial, residential, roadside and agricultural areas. Samples were extracted with hexane by ultrasonic agitation. Extracts were then fractioned on a silica-gel column and the aromatic fraction was analysed by GC-MS. The mean concentration of the total PAH (T-PAH) in the air of Agra was 24.95, 17.95 and 14.25 ng m -3, during winter, monsoon and summer respectively. The average concentration of T-PAH in the soil of Agra was 12.50, 8.25 and 6.44 μg g -1 in winter, monsoon and summer seasons respectively. The aim of this study was to investigate the rate of approach to equilibrium partitioning of PAHs between air and soil compartments and to determine the direction of net flux of the studied PAH between air and soil. Calculated soil-air fugacity quotients indicate that the soil may now be a source of some lighter weight PAHs to the atmosphere, whereas it appears to be still acting as a long-term sink for the heavier weight PAHs to some extent in this region. © 2012 The Royal Society of Chemistry.


Gowda N.,Analytical Research Laboratory | Tekal R.,Analytical Research Laboratory | Thangavelu R.,Analytical Research Laboratory | Vipul K.,M. S. University of Baroda | Rajashree M.,M. S. University of Baroda
Journal of Food and Drug Analysis | Year: 2012

A fast, simple RP-HPLC and three spectrophotometric methods based on classical least squares (CLS), principle component regression (PCR) and partial least squares (PLS) calibrations were developed for simultaneous estimation of ramipril (RAMP) and atorvastatin calcium (ATOR) in formulations. The HPLC assay utilized Phenomenex - Luna RP C-18 (2) 250 x 4.6 mm 5 μm column with mobile phase composition of acetonitrile: 0.1 M sodium perchlorate (pH 2.5) (70 : 30, v/v), and flow rate of 1.5 mL/min with UV detection at 210 nm. Chemometric calibrations were constructed by using absorption data matrix corresponding to concentration data matrix, with measurements in the range of 201-270 nm (Δλ = 1 nm) in their zero-order spectra using 25 samples in training set. The chemometric numerical computations were realized using novel R-Software Environment (version 2.1.1). Reliability of predictions was validated for various ICH regulatory parameters. Both the results of proposed chemometric methods and that of proposed chromatographic method were compared and good agreement was found. Laboratory prepared mixtures and commercial tablet formulations were successfully analyzed by the developed methods.


Mukhopadhyay S.,Analytical Research Laboratory | Kadam K.,Narsee Monjee Institute of Management and Higher Studies | Sawant L.,Narsee Monjee Institute of Management and Higher Studies | Nachane D.,Narsee Monjee Institute of Management and Higher Studies | Pandita N.,Narsee Monjee Institute of Management and Higher Studies
Journal of Pharmacy and Bioallied Sciences | Year: 2011

Objective : Telmisartan is a potent, long-lasting, nonpeptide antagonist of the angiotensin II type-1 (AT 1 ) receptor that is indicated for the treatment of essential hypertension. Hydrochlorothiazide is a widely prescribed diuretic and it is indicated for the treatment of edema, control of essential hypertension and management of diabetes insipidus. In the current article a new, accurate, sensitive, precise, rapid, reversed phase high performance liquid chromatography (RP-HPLC) method was developed for determination of related substances of Telmisartan and Hydrochlorthiazide in tablet dosage form. Materials and Methods : Simultaneous determination of related substances was performed on Kromasil C 18 analytical column (250 4.6 mm; 5m pertical size) column at 40C employing a gradient elution. Mobile phase consisting of solvent A (solution containing 2.0 g of potassium dihydrogen phosphate anhydrous and 1.04 g of Sodium 1-Hexane sulphonic acid monohydrate per liter of water, adjusted to pH 3.0 with orthophosphoric acid) and solvent B (mixture of Acetonitrile: Methanol in the ratio 80:20 v/v) was used at a flow rate of 1.0 ml min 1. UV detection was performed at 270 nm. Results : During method validation parameter such as precision, linearity, accuracy, specificity, limit of detection and quantification were evaluated, which remained within acceptable limits. Conclusions : HPLC analytical method is linear, accurate, precise, robust and specific, being able to separate the main drug from its degradation products. It may find application for the routine analysis of the related substances of both Telmisartan and Hydrochlorthiazide in this combination tablets.


Nabi S.A.,Analytical Research Laboratory | Naushad Mu.,Chemistry Laboratory
Chemical Engineering Journal | Year: 2010

A new inorganic cation exchange material Ti(IV) iodovanadate has been synthesized under a variety of conditions. The experimental parameters such as mixing volume ratio, order of mixing and pH established for the synthesis of the material. The most stable sample has been prepared by adding aqueous mixture of 0.1 M potassium iodate and 0.1 M sodium vanadate into 0.1 M solution (CCl4 medium) of titanium chloride at pH 1. The material is characterized using various analytical techniques like XRD, FTIR, TGA-DTA and SEM. A tentative structural formula has been proposed on the basis of chemical composition, pH titration, FTIR and thermogravimetric analysis. The ion exchange capacity and distribution coefficients of various metal ions have been determined to understand the cation exchange behavior of the material. On the basis of distribution studies, the material was found to be selective for Pb2+ ion. Its selectivity has been examined by achieving some important and analytically difficult binary separations, viz. Cr3+-Pb2+, Fe3+-Pb2+, Zn2+-Pb2+, Cd2+-Pb2+, etc. The material has bee also used as an electron exchange material. The oxidation of Fe(II) to Fe(III) has been achieved by batch-equilibrium technique successfully. The decomposition of hydrogen peroxide by the material has also been studied. The practical utility of Ti(IV) iodovanadate has been demonstrated by separating metal ions quantitatively from a synthetic mixture using the packed column of Ti(IV) iodovanadate. © 2009 Elsevier B.V. All rights reserved.


Alam Z.,Analytical Research Laboratory | Inamuddin,Analytical Research Laboratory | Nabi S.A.,Analytical Research Laboratory
Desalination | Year: 2010

A polymer supported organic-inorganic composite and strongly acidic cation-exchanger polyaniline Ce(IV) molybdate was chemically synthesized and demonstrated to be an excellent ion-exchange material due to its high selectivity for Cd(II), thermal stability and fast elution of an exchangeable H+ ion. The material was characterized for its ion exchange properties to study its cation-exchange behavior. Cd(II) selectivity depends upon the distribution coefficient in several solvent systems. The selectivity of this material varied depending upon its composition and the composition of the eluting solvent. Its selectivity was examined and some important binary separations such as Cd(II)-Pb(II), Cd(II)-Hg(II), Cd(II)-Zn(II) and Cd(II)-Ni(II) were also achieved. The physico-chemical properties of the material were also studied using C, H, N elemental analysis, ICP-MS, FTIR, TGA-DTA, X-ray and SEM studies. © 2009.


Masih A.,Analytical Research Laboratory | Lal J.K.,Analytical Research Laboratory
International Journal of Applied Engineering Research | Year: 2014

Our world is full of synthetic chemicals which pollute water, soil and food. Contamination of groundwater with synthetic organic materials is presenting major environmental problems in many areas of India and in other industrialized nations as well. Polycyclic Aromatic Hydrocarbons (PAHs) are chemicals containing two or more fused benzene rings in a linear, angular or cluster arrangement. PAH contain only carbon and hydrogen. They belong to the group of persistent organic pollutants (POPs) know for there chemical carcinogenicity. They are released into the environment from anthropogenic sources such as combustion of fossil fuels, refused burning; industrial process and motor vehicle exhaust which results in occurrence of PAH in many parts of environment. The concentrations of groundwater PAHs were measured at commercial site of Gorakhpur during winters. The samples were extracted with hexane by ultrasonic agitation. The extracts were then fractioned on a silica-gel column and the aromatic fraction was subjected to high pressure liquid chromatography for analysis of 16 priority PAH pollutants specified by environment protection agency (EPA). Mean concentrations of PAHs were found to be 23.21 ng/L. 3-ring, 4-ring and 5-ring PAHs were found to be dominant in the groundwater of Gorakhpur city having 41%, 19% and 19% of the T-PAH. Univariate Pearson correlation analysis was performed which shows that the sources of all the PAH compounds are somewhat correlated with each other. Use of toxic equivalent factor (TEFs) shows that Ind(123-cd)P contributed the highest carcinogenic exposure equivalent (3.15 ng/L). The levels of PAHs in the groundwater can help in environmental risk assessment of this area. © Research India Publications.


PubMed | Analytical Research Laboratory
Type: Journal Article | Journal: Journal of pharmacy & bioallied sciences | Year: 2011

Telmisartan is a potent, long-lasting, nonpeptide antagonist of the angiotensin II type-1 (AT(1)) receptor that is indicated for the treatment of essential hypertension. Hydrochlorothiazide is a widely prescribed diuretic and it is indicated for the treatment of edema, control of essential hypertension and management of diabetes insipidus. In the current article a new, accurate, sensitive, precise, rapid, reversed phase high performance liquid chromatography (RP-HPLC) method was developed for determination of related substances of Telmisartan and Hydrochlorthiazide in tablet dosage form.Simultaneous determination of related substances was performed on Kromasil C(18) analytical column (250 4.6 mm; 5m pertical size) column at 40C employing a gradient elution. Mobile phase consisting of solvent A (solution containing 2.0 g of potassium dihydrogen phosphate anhydrous and 1.04 g of Sodium 1- Hexane sulphonic acid monohydrate per liter of water, adjusted to pH 3.0 with orthophosphoric acid) and solvent B (mixture of Acetonitrile: Methanol in the ratio 80:20 v/v) was used at a flow rate of 1.0 ml min(-1). UV detection was performed at 270 nm.During method validation parameter such as precision, linearity, accuracy, specificity, limit of detection and quantification were evaluated, which remained within acceptable limits.HPLC analytical method is linear, accurate, precise, robust and specific, being able to separate the main drug from its degradation products. It may find application for the routine analysis of the related substances of both Telmisartan and Hydrochlorthiazide in this combination tablets.


PubMed | Analytical Research Laboratory
Type: Journal Article | Journal: Journal of environmental monitoring : JEM | Year: 2012

Soil is the major environmental reservoir of organic compounds and soil-air exchange is a key process in governing the environmental fate of these compounds on a regional and global scale. Samples of air and soil were collected to study the levels of PAHs in the air and soil of the Agra region. Concentrations of PAH measured at four locations in the city of Agra, covers industrial, residential, roadside and agricultural areas. Samples were extracted with hexane by ultrasonic agitation. Extracts were then fractioned on a silica-gel column and the aromatic fraction was analysed by GC-MS. The mean concentration of the total PAH (T-PAH) in the air of Agra was 24.95, 17.95 and 14.25 ng m(-3), during winter, monsoon and summer respectively. The average concentration of T-PAH in the soil of Agra was 12.50, 8.25 and 6.44 g g(-1) in winter, monsoon and summer seasons respectively. The aim of this study was to investigate the rate of approach to equilibrium partitioning of PAHs between air and soil compartments and to determine the direction of net flux of the studied PAH between air and soil. Calculated soil-air fugacity quotients indicate that the soil may now be a source of some lighter weight PAHs to the atmosphere, whereas it appears to be still acting as a long-term sink for the heavier weight PAHs to some extent in this region.

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