Entity

Time filter

Source Type


Kissane M.,University College Cork | Lawrence S.E.,University College Cork | Maguire A.R.,Analytical and Biological Chemistry Research Facility
Tetrahedron | Year: 2010

1,3-Dipolar cycloadditions of 2-thio-3-chloroacrylamides with nitrile oxides and nitrones is described. A series of novel isoxazolines are isolated from the nitrile oxide cycloadditions, whilst the isoxazolines generated from the nitrone cycloadditions undergo further ring opening to yield piperidines. © 2010 Elsevier Ltd. All rights reserved. Source


Foley D.A.,Analytical and Biological Chemistry Research Facility | O'Connell N.M.,Analytical and Biological Chemistry Research Facility | Mccarthy F.O.,Analytical and Biological Chemistry Research Facility | Maguire A.R.,University College Cork
Journal of Agricultural and Food Chemistry | Year: 2010

Dietary exposure to phytosterols has increased in recent years due to the incorporation of these compounds into cholesterol-lowering products. Previous studies have investigated the cytotoxic effects of the oxidized derivatives of β-sitosterol and determined that phytosterol oxidation products (POP) have a similar but less potent toxicity compared to their cholesterol equivalents. In the present study, the cytotoxicity of the oxidized derivatives of stigmasterol were investigated in the U937 cell line. The stigmasta-5,22-diene-3β, 7β-diol (7β-OH), 5,6-epoxystigmasta-22,23-diol (epoxydiol), 5,6,22,23-diepoxystigmastane (diepoxide), and (22R,23R)-stigmast-5-ene-3β, 22,23-triol (22R,23R-triol) derivatives were identified as the most cytotoxic, and the mode of cell death was identified as apoptosis in cells incubated with 7β-OH, epoxydiol, and diepoxide stigmasterol. The antioxidants R-tocopherol, γ-tocopherol, and β-carotene did not protect against apoptosis induced by 7β-OH and diepoxide stigmasterol; however, R-tocopherol was found to protect against epoxydiolinduced apoptosis. The cellular antioxidant, glutathione, was depleted and the apoptotic protein, Bcl-2, was down-regulated by the stigmasterol oxides identified as apoptotic. © 2010 American Chemical Society. Source

Discover hidden collaborations