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Militello in Val di Catania, Italy

Petralia S.,Analog. MEMS and Sensors | Alessi E.,Analog. MEMS and Sensors | Ventimiglia G.,Analog. MEMS and Sensors
Sensors and Transducers | Year: 2012

Protein coating on solid surface is an essential passivation method to achieve surface biocompatibility for sensing applications. In this paper it was investigated the stability of Bovine Serum Albumine (BSA) coating at different pH values, time and temperature conditions by monitoring the PCR performances on a silicon based lab on chip and by simple contact angle measurement of the coated surface. BSA was deposited on epoxy-silane silicon oxide surface and the coating fully characterized. By means of contact angle values, we found that BSA coating shows surface wettability properties stable under thermal and pH variation, while not treated substrates are very sensible to both pH and temperature stressing. Results here shown indicate that BSA-coated surface is stable in acid, neutral and basic conditions except for pH≥12. © 2012 IFSA. Source

Ventimiglia G.,Analog. MEMS and Sensors | Petralia S.,Analog. MEMS and Sensors | Alessi E.,Analog. MEMS and Sensors
Sensors and Transducers | Year: 2012

An analysis of the oligonucleotides hybridization efficiency and mechanisms, by means of fluorescence detection, on the Lab-on-Chip device provided by STMicroelectronics, is here reported. The device consists of a silicon oxide microfabricated reactor capable to perform a PCR, and fluidically integrated with a microarray detection module aimed to detect the specific amplified DNA, via hybridization, with an assay-specific DNA microarray printed on the silicon oxide surface. The study was performed by using two different silicon surfaces featured by diverse amount of silanol (Si-OH) groups. The study put in evidence the relationship between such type of surface differences with DNA oligonucleotide surface probe density and the device analytical sensitivity. The sensitivity curves and the thermodynamics of a model system have been carried out. © 2012 International Frequency Sensor Association (IFSA). Source

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