Ana Aslan National Institute of Gerontology and Geriatrics

Bucharest, Romania

Ana Aslan National Institute of Gerontology and Geriatrics

Bucharest, Romania
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PubMed | University of Rome La Sapienza, Martin Luther University of Halle Wittenberg, Nencki Institute of Experimental Biology, Leiden University and 22 more.
Type: | Journal: Mechanisms of ageing and development | Year: 2015

Many candidate biomarkers of human ageing have been proposed in the scientific literature but in all cases their variability in cross-sectional studies is considerable, and therefore no single measurement has proven to serve a useful marker to determine, on its own, biological age. A plausible reason for this is the intrinsic multi-causal and multi-system nature of the ageing process. The recently completed MARK-AGE study was a large-scale integrated project supported by the European Commission. The major aim of this project was to conduct a population study comprising about 3200 subjects in order to identify a set of biomarkers of ageing which, as a combination of parameters with appropriate weighting, would measure biological age better than any marker in isolation.


Prada G.I.,Ana Aslan National Institute of Gerontology and Geriatrics | Prada G.I.,University of Bucharest
Acta Endocrinologica | Year: 2013

Over the last decades aging has become a major concern for both medical professionals and policy makers. European Commission has been launching a series of initiatives aimed at drawing attention to great social, political and economic importance of aging research and at finding as well as disseminating the best practices in order to attain a very important objective: to add two years of healthy live to people in Europe by 2020. Nevertheless, there is still controversy regarding the accepted age limit for older people and also concerning various aspects of aging and aging research, most important being the difficulty of correlating chronological age to biological age. A new perspective is suggested and this might help scientists and clinicians understand better the problems of old age and could facilitate targeting research and medical strategies to the most appropriate goals. Several very important projects in the field of aging, funded by European Commission, are presented and a novel approach to the specific situation of older people is offered.


Gradinaru D.,Ana Aslan National Institute of Gerontology and Geriatrics | Gradinaru D.,Carol Davila University of Medicine and Pharmacy | Borsa C.,Ana Aslan National Institute of Gerontology and Geriatrics | Ionescu C.,Ana Aslan National Institute of Gerontology and Geriatrics | And 4 more authors.
Aging Clinical and Experimental Research | Year: 2012

Background and aims: Vitamin D deficiency has been identified in the elderly as a potential risk factor for cardiovascular disease development, possibly through its association with other risk factors, such as type 2 diabetes mellitus (T2DM), hypercholesterolemia and hypertension. The aim of this cross-sectional study was to evaluate the vitamin D status in elderly subjects with impaired fasting glucose (IFG) or T2DM, and to examine its relationships to systemic oxidative stress and biochemical markers of endothelial dysfunction. Methods: Serum 25-hydroxyvitamin D [25(OH)D], fasting glucose, insulin, lipid profile, advanced glycation end products (AGEs), advanced oxidation protein products (AOPPs), low-density lipoprotein susceptibility to oxidation (oxLDL) and nitric oxide metabolic pathway products (NOx) were analyzed in elderly subjects with IFG (n=30) and T2DM (n=35) compared with aged-matched controls (n=25). Results: 25(OH)D levels in the IFG and T2DM groups were significantly lower than in controls (31.9±1.9 and 28.5±1.9 vs 39.4±2.4 ng/mL, p<0.001), and associated with significantly (p<0.001) higher levels of the oxidative stress parameters AGEs, AOPPs, oxLDL and NOx. Hypovitaminosis D [25(OH)D)<30 ng/ml] markedly enhanced the oxidative stress and cardiovascular risk in hyperglycemic subjects compared with sufficient vitamin D [25(OH)D)≥30 ng/mL] status subjects. In subjects with IFG and T2DM (n=65), the vitamin D status was significantly inversely correlated both with oxLDL (r=-0.413, p =0.001) and AOPPs (r=-0.475, p<0.001), and strongly positively associated with high-density lipoprotein cholesterol (r=0.609, p <0.001). Conclusions: In the elderly with impaired glucose metabolism the vitamin D status is inversely associated with levels of circulating markers of oxidative stress and endothelial dysfunction, especially in subjects with hypovitaminosis D. ©2012, Editrice Kurtis.


Diaconeasa A.G.,Polytechnic University of Bucharest | Rachita M.,Ana Aslan National Institute of Gerontology and Geriatrics | Stefan-van Staden R.-I.,Polytechnic University of Bucharest | Stefan-van Staden R.-I.,Romanian National Institute for Research for Electrochemistry and Condensed Matter
Medical Hypotheses | Year: 2015

Introduction and aims: There are over 300 hypotheses of aging, but none of them has enough predictive power to explain most experiments and observations on this process. On the basis of a critical analysis of the most relevant data on aging, especially on the factors that influences its rhythm, we present a new hypothesis, as well as the way the hypothesis' predictions explain some of the phylogenetic implications of the aging process. Methods: The hypothesis starts from a new, biochemical view on evolution and the behavior of living matter. According to this view, life is a fabric of chemical reactions that sustain each other. Reactants and energy support are needed for these reactions to take place in a cell. Given this, aging stems from a leftward shift of the global equilibrium of some biochemical reactions involved in cell differentiation and repair, which take place at a high level during the organism's growth period. In time, for species with evident aging, some reactions lose their specificity, which affects cell division and differentiation. This, in turn, influences cell energy metabolism. Results: Cell and tissue degeneration appears when, while some specific reactions are absent, non-specific reactions such as those of cell proliferation receive additional energy support. Conclusions: This hypothesis explains phylogenetic differences related to lifespan and longevity, and body-size differences between species and within the same species. © 2015 Elsevier Ltd.


Gradinaru D.,Ana Aslan National Institute of Gerontology and Geriatrics | Gradinaru D.,Carol Davila University of Medicine and Pharmacy | Borsa C.,Ana Aslan National Institute of Gerontology and Geriatrics | Ionescu C.,Ana Aslan National Institute of Gerontology and Geriatrics | And 2 more authors.
Mechanisms of Ageing and Development | Year: 2015

Oxidized LDL (oxLDL) and nitric oxide (NO) exert contradictory actions within the vascular endothelium microenvironment influencing key events in atherogenesis. OxLDL and NO are so far regarded as representative parameters of oxidative stress and endothelial dysfunction, new targets in prevention, diagnosis and therapy of cardiovascular diseases, and also as candidate biomarkers in evaluating the human biological age. The aim of this review is to explore recent literature on molecular mechanisms and pathophysiological relationships between LDL oxidation, NO synthesis and vascular endothelium function/dysfunction in ageing, focusing on the following aspects: (1) the impact of metabolic status on both LDL oxidation and NO synthesis in relation with oxidative stress, (2) the use of oxidized LDL and NO activity as biomarkers in human studies reporting on cardiovascular outcomes, and (3) evidences supporting the importance of oxidized LDL and NO activity as relevant biomarkers in vascular ageing and age-related diseases. © 2015 The Authors.


Gradinaru D.,Ana Aslan National Institute of Gerontology and Geriatrics | Gradinaru D.,Carol Davila University of Medicine and Pharmacy | Borsa C.,Ana Aslan National Institute of Gerontology and Geriatrics | Ionescu C.,Ana Aslan National Institute of Gerontology and Geriatrics | Margina D.,Carol Davila University of Medicine and Pharmacy
Journal of Proteomics | Year: 2013

We aimed to explore the association of advanced oxidation and advanced glycation of proteins, and their interrelations with endothelial nitric oxide synthesis, oxidative stress, metabolic profile as well as other atherosclerotic risk markers in prediabetic and diabetic elderly subjects. Advanced glycation end products (AGEs), advanced oxidation protein products (AOPPs), low-density lipoprotein susceptibility to oxidation (oxLDL) and nitric oxide metabolic pathway products (NOx) were assessed in subjects with impaired fasting glucose (prediabetes, IFG; n = 90), and type 2 diabetes mellitus (T2DM, n = 95) versus control subjects (n = 88). Higher levels of AOPPs, AGEs, oxLDL, NOx, atherosclerosis risk markers, and insulin resistance were pointed out in IFG and T2DM groups compared with control. Strong positive associations (p. <. 0.01) of AGEs with fasting glucose and HbA1c were found in both hyperglycemic groups, whereas AOPPs were significantly correlated (p. <. 0.01) only in T2DM. In T2DM, AGEs and AOPPs significantly (p. <. 0.01) correlated with insulin resistance index HOMA-IR, oxLDL and small LDL particle size (TG/HDL-C), and positively with NOx. Direct associations of AGEs and AOPPs with TC/HDL-C and oxLDL/HDL-C, and AGEs-AOPPs interrelations (p. <. 0.01) were identified in IFG and T2DM groups. AGEs and AOPPs in combination with oxLDL and NOx could be important biomarkers for evaluating the association between diabetes and atherosclerotic disorders in aging diabetic patients. Biological significanceIn the present study we have made an attempt to approach the biological and clinical significance of the oxidative and glycoxidative protein damage, in subjects with prediabetes and type-2 diabetes mellitus. AGEs and AOPPs in combination with oxLDL and NOx appear to be important biomarkers for evaluating the association between diabetes and atherosclerotic disorders in aging diabetic patients. More importantly, this cluster of biomarkers that links the short term, "real time" metabolic impairment parameters (NOx, serum glucose, HOMA-IR, serum lipid profile) and the "metabolic memory" markers resulting from the long-term hyperglycemia and hyperlipidemia-induced oxidative stress (HbA1c, AGEs, AOPPs and oxLDL), could be valuable in predicting not only vascular complications in T2DM, but also the onset of diabetes, hence enabling therapeutic interventions from the early stages of diabetes. This article is part of a Special Issue entitled: Posttranslational Protein modifications in biology and Medicine. © 2013 Elsevier B.V.


Olaru O.G.J.,Carol Davila University of Medicine and Pharmacy | Pena C.M.C.,Ana Aslan National Institute of Gerontology and Geriatrics
Revista Romana de Medicina de Laborator | Year: 2013

Discovery of new biomarkers or panels of biomarkers for early diagnosis of ovarian cancer is one of the great challenges of modern medicine. The use of determination of CA125 (the most commonly used biomarker) was improved by taking into account the dynamic values available in the context of screening. Currently, the highest sensitivity of screening tests performed for early diagnosis of ovarian cancer is 96% and was obtained using a panel of serum biomarkers composed of apolipoprotein A1, transthyretin, transferrin and CA125. This paper presents different types of serum proteins that are currently studied, and should be proposed as new biomarkers for detecting ovarian cancer. Future research needs to be conducted in order to find a new tumor marker panel that is quantitative, reproducible, and fast and has an even higher sensitivity and specificity for the early detection of ovarian cancer.


PubMed | Ana Aslan National Institute of Gerontology and Geriatrics and Carol Davila University of Medicine and Pharmacy
Type: | Journal: Mechanisms of ageing and development | Year: 2015

Oxidized LDL (oxLDL) and nitric oxide (NO) exert contradictory actions within the vascular endothelium microenvironment influencing key events in atherogenesis. OxLDL and NO are so far regarded as representative parameters of oxidative stress and endothelial dysfunction, new targets in prevention, diagnosis and therapy of cardiovascular diseases, and also as candidate biomarkers in evaluating the human biological age. The aim of this review is to explore recent literature on molecular mechanisms and pathophysiological relationships between LDL oxidation, NO synthesis and vascular endothelium function/dysfunction in ageing, focusing on the following aspects: (1) the impact of metabolic status on both LDL oxidation and NO synthesis in relation with oxidative stress, (2) the use of oxidized LDL and NO activity as biomarkers in human studies reporting on cardiovascular outcomes, and (3) evidences supporting the importance of oxidized LDL and NO activity as relevant biomarkers in vascular ageing and age-related diseases.


PubMed | Ana Aslan National Institute of Gerontology and Geriatrics
Type: Journal Article | Journal: Aging clinical and experimental research | Year: 2016

Dementia is one of the most disabling conditions associated with old age. With increasing life expectancy, prevalence of both dementia and diabetes is rising. The complex pathological relationship between diabetes mellitus (DM) and dementia has been studied, but is not yet fully understood.The main objective of this study was to investigate the relationship between metabolic parameters and the cognitive function in older diabetics.A total number of 360 diabetic subjects, age 65years and over, and 300 older people controls were included. Clinical and biological parameters, together with the cognitive function, were assessed every 6months over a 18-month period, for each study participant.By employing a multivariate linear regression analysis, several significant relationships have been identified: between Clock Drawing Test (CDT) scores and HbA1c (R The close detailed monitoring of the cognitive function and a rigorous metabolic control are important, especially in the very early stages of DM. Addressing factors such as weight control in older diabetic patients could contribute to better cognitive outcomes.


PubMed | Ana Aslan National Institute of Gerontology and Geriatrics and Carol Davila University of Medicine and Pharmacy
Type: Journal Article | Journal: Acta physiologica Hungarica | Year: 2015

Erythropoietin (EPO), a key hormone involved in red blood cell formation has been recently acknowledged for its pleiotropic actions and protective role in ageing and various pathological conditions concurrent with oxidative stress, vascular diseases and metabolic abnormalities such as diabetes mellitus. The aim of the study was to evaluate the relationship between circulating erythropoietin levels and oxidative stress biomarkers, in elderly with type 2 diabetes (T2DM). The study was carried out in 67 subjects with T2DM (69 5 years; n = 37) without anemia, and aged-matched controls (70 6 years; n = 30). EPO serum levels, erythrocyte susceptibility to lipid peroxidation (ESP) and total antioxidant capacity (TAC) were evaluated. Lower EPO levels (p < 0.01) and higher ESP values (p < 0.001) were found in T2DM group, compared to healthy subjects. EPO levels showed significant negative associations with ESP, both in T2DM subjects (r = -0.565; p < 0.001) and in all study population (r = -0,600; p < 0,001; n = 67). In conclusion, we provide new data regarding the cytoprotective effect of EPO exerted at systemic level on erythrocyte membrane, in the particular state of impaired glucose metabolism associated with oxidative stress, in the elderly.

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